The antibody clone TDP2/1258 targets the protein known as ETS1-Associated Protein II (EAPII), also called Tyrosyl-DNA Phosphodiesterase 2 (TDP2). TDP2 is a multifunctional enzyme of interest in DNA repair, signal transduction and transcriptional regulation. This mouse monoclonal antibody is engineered to provide specific, reliable detection of TDP2/EAPII in research applications. The product is available from Genprice at the link : Mouse Monoclonal ETS1 Associated Protein II Antibody (TDP2/1258) – Azide Free

What is EAPII / TDP2 ?

EAPII (ETS1-Associated Protein II) was first described as a protein interacting with the transcription factor ETS1 (and related ETS family members). It has alternate names including TDP2 (Tyrosyl-DNA Phosphodiesterase 2). NCBI

TDP2 (NCBI) is an enzyme in the superfamily of divalent cation‐dependent phosphodiesterases. Its functional importance lies in repairing DNA when topoisomerase II (TOP2) is covalently trapped on DNA ends (via a 5’-tyrosyl-DNA bond). It hydrolyses the 5’-phosphodiester (5’-TYR) linkage to free a clean 5’ phosphate terminus.

Beyond DNA repair, TDP2/EAPII has roles in signal transduction. For example, it associates with CD40, TNF receptor-75 and TRAFs, and is reported to inhibit NF-κB activation under some conditions.

Why it matters to research ?

Because TDP2 intersects with DNA repair and transcription factor regulation, it is relevant in fields including oncology, neurobiology, virology, and signal-transduction research. It is also of interest in the study of protein‐DNA adducts, topoisomerase poisons (e.g., in chemotherapy), and cellular responses to DNA damage. Having a high‐quality antibody allows researchers to localize TDP2, study its expression levels, explore its regulation and evaluate its subcellular distribution.

Key specifications

1. Clone: TDP2/1258.
2. Host: Mouse monoclonal; isotype IgG2b κ.
3. Target antigen: Human TDP2 / EAPII.
4. Molecular target size: Approximately 41 kDa.
5. Format: Available in azide-free formulations (important for sensitive downstream applications) per Genprice listing.
6. Applications: Validated for IHC-P (immunohistochemistry on paraffin sections), ICC/IF (immunocytochemistry/immunofluorescence), Flow Cytometry (FCM), and Western Blot (WB) in some variants.

Product advantages

1. The clone TDP2/1258 is widely referenced across multiple suppliers, suggesting robustness and reproducibility.
2. Mouse monoclonal format offers consistency from batch to batch, which is beneficial for quantification or longitudinal studies.
3. Azide-free formulation helps avoid interference in assays sensitive to preservatives (e.g., live cell staining, mass cytometry).
4. Broader application compatibility (IHC, IF, FCM) expands utility across tissue‐based and single‐cell studies.

Immunohistochemistry (IHC)

1. Researchers can use this antibody for IHC on paraffin‐embedded human tissue samples. For example, human lung, prostate or colon carcinoma sections have been stained with this antibody.
2. Tip: Heat-mediated antigen retrieval (e.g., Tris/EDTA pH 9.0) is recommended per supplier data.

Immunofluorescence and ICC

1. In cell culture systems, the antibody enables localization of TDP2 within nuclear and/or cytoplasmic compartments. Some sources indicate the antibody recognizes both nuclear and cytoplasmic pools.

Flow Cytometry (FCM)

1. For single‐cell analysis, the antibody has been validated in flow cytometry applications (dilution typically 0.5-1 µg per 10^6 cells).

Western Blot (WB)

1. The antibody detects the ~41 kDa TDP2 protein band under denaturing SDS-PAGE conditions. Researchers should verify specificity by including knockdown/knockout controls if possible.

The Mouse Monoclonal ETS1 Associated Protein II Antibody (clone TDP2/1258) is a versatile reagent targeting the key DNA repair and transcription-regulatory protein TDP2/EAPII. It offers strong application flexibility (IHC, IF, FCM, WB), high specificity and is available in azide-free formulation for advanced workflows. Researchers working in DNA damage signaling, transcription factor interaction, oncology, or advanced cell biology will find this antibody a reliable tool. With proper controls, optimization and interpretation, it enables confident exploration of TDP2 biology.