DESCRIPTION
The Malachite Green Phosphate Assay Kit is based on quantification of the
green complex formed between Malachite Green, molybdate and free
orthophosphate. The rapid color formation from the reaction can be
conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate
reader. The non-radioactive colorimetric assay kits have been optimized to
offer superior sensitivity and prolonged shelf life. The assay is simple and
fast, involving a single addition step for phosphate determination. Assays
can be performed in tubes, cuvettes or multi-well plates. The assays can be
conveniently performed in 96- and 384-well plates for high-throughput
screening of enzyme inhibitors.
|