VitoTOX System

Vitotox is a high-throughput biolumine-scent assay that offers a unique method for rapid and cost effective geno- and cytotoxicity screening.

Unique assay principle
The Vitotox assay is a Salmonella typhimurium test that uses the light emission of bacteria to detect the genotoxicity, cytotoxicity and muta-genic potency of the sample. It is used for the detection of genetic damage caused by the chemical in pharmaceutical, cosmetic, environ-mental, etc., research. The assay is based on a reporter gene system where luciferase activity is used as a function of the genotoxicity. Luciferase expression is activated via a cascade of reactions known as the SOS response.
Benefits of the assay
The entire DNA content of the cell functions as a target for the genotoxin
to display its effect. Therefore, only a few micrograms of the sample is normally required for the assay.
A cytotoxicity assay is also per-formed together with each sample to prevent false positive and nega-tive results.
No cell growth is required for the genotoxicity detection, resulting in very short assay times.

 General principle of SOS induction. Derepression of the RecN gene isfollowed by placing a Lux reporter system under transcriptional control of the RecN promoter. A turnkey solution for the CellularAssay Workstation!

96-well layout for the Vitotox test
 

The test uses a simple “mix and measure” procedure: the com-pounds are pipetted into 96- or 384-well microplates as a dilution series along with blanks, positive control samples, and with or without S9 metabolic extract. The cultured test bacteria are added and the light emission is followed over a three-hour assay period.
Correlation with traditional genotox-icity assays is exceptionally good.

ATP Assay Products
The Quantitative ATP Monitoring Kit is used for measur-ing free ATP concentrations. Using the bioluminescence technique, the monitoring kit measures ATP in bacterial, plant or mammalian cells, as well as tissue samples over a concentration range of 10-11 to 10-6 moles per liter. This kit also enables any enzyme or substrate that can be coupled to the production or consumption of ATP to be mea-sured quantitatively. The assay can be freely used with any cells and cell lysis reagents. It is convenient, rapid and reproducible, with results available in just minutes. Individual assay components are also avail-able for those researchers who only have very few samples.

http://www.bioxys.com/i_Biotium/bioscience_assay_kits.htm