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Arginine Vasopressin
Receptors (V1a, V1b, and V2) Antibodies
Vasopressin (AVP,
Arginine-8-Vasopressin), the antidiuretic hormone is cyclic nonapeptide
invovled in the homeostasis of body fluid osmolality, blood volume, vascular
tone, and blood pressure. Specific actions of AVP include inhibition of
diuresis, contraction of smooth muscle, stimulation of liver glycogenesis,
and modulation of ACTH release from pituitary. AVP belongs to the family of
vasocative and mitogenic peptide invovled in normal and pathological cell
growth and differentiation.
AVP exerts its action through binding to
specific membrane receptors coupled to distinct second messengers. There are
3 types of AVP receptors: V1a, V1b, and V2
subtypes. The V2 receptor stimulates adenyl cyclase and protein
Kinase A, V1 activate phospholipase A2.C, and D, resulting into production
of IP3 and DAG, the mobilization of intracellular calcium, the influx of
extracellular calcium, the activation of protein Kinase C, and protein
phosphorylation. The V1a receptors mediate vasoconstriction and hepatic
gluconeogenesis platelet aggregation, coagulation factor release. V1a
receptors are found in vascular smooth muscle, hepatocytes, blood platelets,
lymphocytes and monocyte, type II pneumocytes, adrenal cortex, brain,
reproductive organs, retinal epithelium, renal mesangial cells.
The V1b modulates ACTH secretion from the
anterior pituitary and the V2 mediates anti-diuretic effects. V1b receptors
are located in anterior pituitary. V2 renal receptors are present in
medullary portion of the kidney where they control free water and urea
adsorption by stimulating adenyl cyclase.
AVP receptors are members of the G-protein
coupled receptors with putative 7 transmembrane domains. The sizes of
various AVP receptors are V1a (rat,
424 AA; human, 418 AA); V1b (rat, 421
AA; human, 424 AA); V2 (rat, 371 AA;
human 371 AA). The N-terminus and C-terminus are predicted to be
extracellular and cytoplasmic, respectively.
ADI has produced rabbit-antibodies to AVP
V1a, V1b, and V2 receptors using peptide sequences specific to each protein.
These antibodies should be useful in studying specific AVP receptors.
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Items |
Peptide
Antigen |
Ab
host |
Ab
Cross reactivity |
Neat
Antisera
Cat #
(100 ul) |
Aff.
Pure
Ab Cat #
(100 |
* Control
Peptide Cat#
(100 ug) |
|
rV1a (Ab #1) |
r, 19 aa ~ EC1 |
rb |
r, m
(h, s, p?) |
AVP1A11-S |
AVP1A11-A |
AVP1A11-P |
|
rV1a (Ab #2) |
r, 18 aa ~ CP4 |
rb |
r (m, h?) |
AVP1A12-S |
AVP1A12-A |
AVP1A12-P |
|
rV1b |
r, 18 aa, ~ EC1 |
rb |
r, h |
AVP1B13-S |
AVP1B13-A |
AVP1B13-P |
|
rV2 |
r, 21 aa, ~CP4 |
rb |
r, m, h |
AVPV21-S |
AVPV21-A |
AVPV21-P |
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Control Rabbit IgG
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For use as control in ELISA,
Western, immunohistochemistry, etc.
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20009-1
(1 mg)
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m = mouse; r=rat; h=human; s=sheep; p=pig; rb=rabbit; EC=Extracellular
domain; CP=Cytoplasmic domain;
"Neat Antisera"
are the unpurified antiserum and it is suitable for ELISA and
Western.
"Affinity pure"
antibodies have been over the antigen-affinity column
and recommended for immunohistochemical applications.
"Control peptides"
can not be used for Western as they are very short peptides.
They are intended for ELISA or antibody competition studies.
All Products are for in vitro
research use only.
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