Ubiquitin Activating Enzyme (E1) is responsible for the first step in ubiquitin-protein isopeptide bond formation.

E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner.

Manufacturing: Proteome Resources baculoviral expression in insect cells (Sf9, Spodoptera frugiperda). Form: Solution in 20% glycerol, 150mM NaCl, 5mM DTT, 75mM Tris-HCl, pH 8.0.

Concentration: 0.5 µg/µl. Purity: >=98% (SDS-PAGE). Molecular Weight: 140 kDa. Storage: -70°C. Avoid freeze/thaw cycles.

Shipping: In dry ice. References: Ciechanover, A., et al. 1982. J. Biol. Chem. 257, 2537. Haas, A.L. and Rose, I.A. 1982. J. Biol. Chem. 257, 10329. Ciechanover, A., et al. 1981. Proc. Natl. Acad. Sci. 78, 761.