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Ubiquitin Activating Enzyme (E1) : is responsible for the first
step in ubiquitin-protein isopeptide bond
formation. E1 catalyzes the activation
of the C-terminal carboxyl group of ubiquitin by forming a
high-energy thioester bond in an ATP-dependent
manner.
Manufacturing: Proteome Resources baculoviral expression in
insect cells (Sf9, Spodoptera frugiperda). Form: Solution in 20%
glycerol, 150mM NaCl, 5mM DTT, 75mM Tris-HCl, pH 8.0.
Concentration: 0.5 µg/µl. Purity: >=98% (SDS-PAGE). Molecular
Weight: 140 kDa. Storage: -70°C. Avoid freeze/thaw cycles.
Shipping: In dry ice. References: Ciechanover, A., et al. 1982.
J. Biol. Chem. 257, 2537. Haas, A.L. and Rose, I.A. 1982. J.
Biol. Chem. 257, 10329. Ciechanover, A., et al. 1981. Proc.
Natl. Acad. Sci. 78, 761. |
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