Cat. # TSC11-P, Rat TSC Control Peptide, SIZE: 100 ug/100 ul
Cat. # TSC11-S, Rabbit Anti-rat TSC antiserum, SIZE: 100 ul
Cat. # TSC11-A, Rabbit Anti-rat TSC antiserum (aff pure), SIZE: 100 ug

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells - the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+-Cl-cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+-Cl--cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.

In the distal convoluted tubule (DCT) of the kidney, the primary apical entry pathway for Na is via the thiazide-sensitive Na-Cl transporter (TSC or NCC; human 1021 aa, rat/mouse 1002 aa; ~60 % identity with NKCC). TSC is specifically detected at the apical cell membrane of DCT.

Source of Antigen and Antibodies

Antigen: 18aa peptide of Rat TSC; Designated (TSC11-P).
Location: ~N-terminus, Cytoplasmic
Ab Host/type: Rabbit, polyclonal
Ab Format: Aff pure IgG (cat #TSC11-A)

Recommended Usage

Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure using Chemiluminescence technique).

ELISA (1:10K-1:100K; using 50-100 ng of control peptide/well).

Histochemistry & Immunofluorescence: Not tested. We recommend the use of affinity purified antibody at 2-20 ug/ml.

Specificity & Cross-reactivity

The 18 AA rat TSC11-P control peptide is 88% conserved in mouse and rabbit, and 83% in human TSC. No significant sequence homology is detected with other CCC. Antibody cross-reactivity in various species has not been studied. The TSC11-P control peptide, because of its small size (~2 kDa), is not suitable for Western. It should be used in ELISA, dot blot or to neutralize antibody and confirm specificity of antibody.

References:

(1). Gamba G et al (1994) J Biol. Chem. 269, 17713-17722; Simon DB et al (1996) Nature Genet. 12, 24-30; Mount DB et al (1995) J Am. Soc. Nephrol. 6, 347; Igarashi P et al (1995) Am. J. Physiol. 269, F405-418; Haas M et al (1987) Am. J. Physiol. 253, C243; Mount DB et al (1998) J. Exptl. Biol. 201, 2091 (review).