Topoisomerase DNA repair

DNA Synthesis and Repair Proteins Antibodies

ADI has produced highly specific rabbit polyclonal and mouse monoclonal antibodies to various proteins involved in DNA Synthesis and repair.

Items	Antigen/ peptide location	Ab Host	*Expected Ab Crossreactivity	Antiserum Cat # (100 ul)	Aff. Pure /Mono Cat # (100 ug)	* Control Peptide Cat# (100 ug)
Ape ab #1	h, Apex protein	Rb, poly	h (m, r?)	APE11-S	.	.
APE pure protein		Human Recombinant pure APE protein for WB (inactive), Cat # APE11-C (100 ul) Human Recombinant pure APE protein (Active), Cat # APE25R-5 (5 ug); 10 ug
APE ab # 2	M, APE protein	Rb, poly	m, r (h?)	APE12-S	.	.
hOGG1 ab # 2	h, OGG1 protein	Rb, poly	h ,m, r	HOGG12-S	.	.
mOGG1 ab # 3	m, 18 aa, NT	Rb, poly	m, r	MOGG13-S	MOGG13-A	MOGG13-P
mOGG1 pure protein		Mouse Recombinant pure mOGG1 protein for WB (inactive), Cat # MOGG13-C (100 ul) Mouse Recombinant pure mOGG1 protein (Active), Cat # mOGG25R-5 (5 ug); 10 ug
hNTH1 ab # 1	H, NTh1 protein	Rb, poly	H (m, r?)	HNTH11-S	.	.
MGMT ab # 1	H, MGMT protein	Rb, poly	H (m, r?)	MGMT11-S	MGMT11-A	MGMT11-P
MGMT ab # 2	H, MGMT protein	M, mono	H (m, r?)		MGMT12-M	.
MGMT pure protein		Human Recombinant pure MGMT protein for WB (inactive), Cat # MGMT12-C (100 ul) Human Recombinant pure MGMT protein (Active), Cat # MGMT15-R-10 (5 ug); 10 ug
mutY homolog ab # 1	H, mutY, 17 aa, NT	Rb, poly	H (m, r?)	MUTY11-S	MUTY11-A	MUTY11-P
mutY homolog ab # 2	H, mutY, 19 aa, CT	Rb, poly	H (m, r?)	MUTY12-S	MUTY12-A	MUTY12-P
dsDNA ab # 1	dsDNA	M, mono	all species	.	DNA11-M
DNA Ligase ab # 1	b, DNA Lig. Protein	M, mono	H, b (m, r?)		DNAL11-M
DNA Pol. beta ab # 1	r, DNA Pol. b Protein	M, mono	r, m, h		DNPB11-M
DNA Pol. epsilon ab # 1	h, DNA Pol. e 1-176 aa	M, mono	r, m, h		DNPE12-M
DNA Pol. gamma ab # 1	h, DNA Pol. g26-276 aa	M, mono	r, m, h		DNPG13-M
DNA Pol delta ab # 1	h 21-aa ~NT	Rb, poly	r, m, h, b	DNPD11-S		DNPS11-P
DNA Primase ab # 1	m, p49 subunit	Rb, poly	m, h	.	DNPR13-A
DNA Primase ab # 1	m, p58 subunit	M, mono	m, h	.	DNPR14-A
DNA Pkcs ab # 1	h, DNA PK	M, mono	h		DNPK11-M
DNAse I	bovine, DNAse I	G, poly	bovine (m, r, h?)	.	DNASE11-A	DNASE11-C (100 ul)
DNAse II ab # 1	h 21-aa ~CT	Rb, poly	h	DNASE21-S	DNASE21-A	DNASE21-P
DNAse II ab # 2	m 17-aa ~CT	Rb, poly	m	DNASE22-S	DNASE22-A	DNASE22-P
Ku70 ab # 1	h, Ku 506-541 aa	M, mono	h		Ku701-M
Ku80 ab # 1	h, Ku 1-374	M, mono	h		Ku801-M
ERCC1 ab # 1	h, ERCC1	M, mono	h (m, r?)		ERCC11-M
ORC1 ab # 1	h, ORC1	M, mono	h (m, r?)		ORC11-M
XPA ab # 1	h, XPA	M, mono	h (m, r?)		XPA11-M
XPG/ERCC5 ab # 1	h, XPG	M, mono	h (m, r?)		XPG11-M
XRCC1 ab # 1	h, XRCC1 protein	M, mono	h (m, r?)		XRCC11-M
Thymine Glycols ab # 1	Mrl autoimmune mice	M, mono	h, m, r		THG11-M
Topoisomerase II a, ab # 1	H, 1513-1530 aa	M, mono	h, m, r		TOP11-M
Thymidylate Synthase, ab # 1	H, TS protein	M, mono	H (m, r?)		TS11-M
Thymidine Phosphoryl, ab # 1	H, TP protein	M, mono	M,		THP11-M
Control (non-immune) IgGs for ELISA/Western/IHC etc		Control Rabbit IgG, Cat #20009; 1 mg Control Goat IgG, Cat #20011; 1 mg Control Mouse IgG, Cat #20008; 1 mg

m=mouse; r=rat; h=human; b=bovine; d=dog; ~CT or ~NT=near C or N-terminus. EC=Extracellular; CP=Cytoplasmic domain;

* Expected antibody crossreactivity information is mostly based upon high (>70%) sequence conservation of antigenic/control peptides in various species. When antibody crossreactivity has actually been experimentally confirmed in various species, it will be mentioned in the appropriate data sheets.

"Neat Antisera or antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" IgG may be more suitable for immunohistochemical (IHC) applications and to reduce background in most immunological applications including ELISA and Western.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody blocking studies to establish antibody specificity.
Western blot +ve protein controls, where available, are semi-pure, pure or recombinant proteins that are formulated in SDS-PAGE sample buffer (reduced). They are recommended to be used for Western (load 10 ul/lane) for visulization with antibodies.

All Products are for in vitro research use only. rev 40415A

List of publications using ADI's Antibodies to various products involved in DNA Repair

MGMT Biswas T 1999 Activation of human O6-methylguanine-DNA methyltransferase gene by glucocorticoid hormone Oncogene18, 525 - 532 WB, MGMT protein antibodies.

hMYH Boldogh, Istvan 2001 hMYH cell cycle-dependent expression, subcellular localization and association with replication foci: evidence suggesting replication-coupled repair of adenine:8-oxoguanine mispairs Nucleic Acids Research, 2001, Vol. 29, No. 13 2802-2809 WB other mutY from Dr Mitra, PCNA from SC.

hNTH1 Liu X and R Roy 2002 Truncation of Amino-terminal Tail Stimulates Activity of Human Endonuclease III (hNTH1) J. Mol. Biol. 321, 265-276 WB custom antibodies to proteins.

MYH # 2 ab Yamane A 2003 Suppressive activities of OGG1 and MYH proteins against G:C to T:A mutations caused by 8-hydroxyguanine but not by benzo[[!LB]]a[[!RB]]pyrene diol epoxide in human cells in vivo,
Carcinogenesis, Apr 2003; 561. WB,, ~38 kda and others, ape-1 form novus.

MYH # 2 ab Yamaguchi S 2002 A single nucleotide polymorphism at the splice donor site of the human MYH base excision repair gene results in reduced translation efficiency of its transcripts Genes to Cells 7 Issue 5 Page 461 WB,, ~38 kda and others , ape-1 form novus.

hOGG1 Cardozo-Pelaez F eat al 2002 Effects of diethylmaleate on DNA damage and repair in the mouse brain Free Radical Biology and Medicine 33, 292-298 WB,, ~38 kda and others mouse brain.

hOGG1 Stedeford, Todd 2001 Comparison of base-excision repair capacity in proliferating and differentiated PC 12 cells following acute challenge with dieldrin Free Radical Biol Med 31, 1272-1278 WB, pC12 cells.

hOGG1 Cappelli E et al 2001 Rates of base excision repair are not solely dependent on levels of initiating enzymes Carcinogenesis 2001 22: 387-393 WB,, ~38 kda and others, ape-1 form novus.

hOGG1 Hyun J-W 2000 Leukemic cell line, KG-1 has a functional loss of hOGG1 enzyme due to a point mutation and 8-hydroxydeoxyguanosine can kill KG-1 Oncogene19, 4476 - 4479 WB,, ~38 kda and others.