Source of Antigen and Antibodies

Purified rat tyrosine hydroxylase (TH) was injected into Balb/c mice to produced mouse monoclonal antibodies.

Form & Storage

Antiserum is supplied as neat ascites (100 ul soln or lyophilized) in 0.02% sodium Merthiolate or 0.1% sodium azide as preservative.

Lyophilized products should be reconstituted in 100 ul water and gently mixed for 15 min at room temp. All antibody received in solution or lyophilized forms should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Specificity and crossreactivity

Antibody # 1, produced by clone XSM-1 (IgG1) recognizes the N-terminal (1-20 aa) region of rat TH and it recognizes the intact TH protein. The monoclonal antibody has broad species crossreactivity. Antibodies recognize TH from rat, mouse, human, bovine, monkey, sheet, G. Pig, and rabbit.

Recommended Usage

Western Blotting (1:1K-5K) using Chemiluminescence substrates.

Histochemistry & Immunofluorescence: We recommend an initial dilution of 1:500 to 1:1K. Users must optimize antibody dilution based upon their specific requirement.

Immunoprecipitation

Antibodies can be used to immunoprecipitate both native and denatured TH. Use 10-20 ul antibody per 100-200 ug of protein extracts. Users must optimize antibody dilution based upon their specific requirement.

*This product is for In vitro research use only.