TX-HYB Hybridoma Growth Supplement

As you may have heard, the FC-210001 ORIGEN Hybridoma Cloning factor is no
longer available.
GENTAUR is pleased to inform you that we have a replacement for the FC210001
ORIGEN Hybridoma Cloning factor.
We have an alternative product which enables you to easily prepare your own
Serum-Free Hybridoma Growth Medium.
The
TX-HYB
Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth
factors used in hybridoma production. TX-HYB increases the yield of hybridomas
surviving HAT selection, the cloning efficiency of B-cell hybridomas, and the
number of antibody producing colonies.
The TX-HYB Hybridoma Growth Supplement is ready to use. Prepare the serum free
Hybridoma growth medium by dissolving 1 bottle of TX-HYB (11 ml) into 1 Liter of
D-MEM F-12 (Dulbecco's Modified Eagle's Medium F-12).
The
prepared Serum-Free
Hybridoma growth medium is a chemically defined
culture medium supporting the
serum-free growth
of various hybridomas and 293 cells. The compounds of Hybridoma growth medium
include water for injection, a modified DMEM/F12 base, HEPES buffer, known
amounts of insulin, transferin, testosterone, sodium selenite, ethanolamine, a
variety of saturated and unsaturated fatty acids, and stabilizing proteins.
TX-HYB Hybridoma growth supplement contains no bovine serum albumine or other
undefined protein mixtures.
TX-HYB Hybridoma Growth Supplement, 11 ml bottles (for 1 Liter of Hybridoma
Growth Medium)
1 bottle of TX-HYB for 57 Euro.
5 bottles of TX-HYB for 270 Euro.
10 bottles of TX-HYB for 510 Euro.
You can find the datasheet and the protocol here:
Datasheet.pdf
Protocol.pdf