DESCRIPTION
The SuperLight Luciferase Reporter Gene Assay is based on the quantitation
of luciferase expression in mammalian cells, yeast or E. coil cells, using
luciferin and ATP as substrates. The reaction results in light production
which can be conveniently measured by a luminometer.
Reaction scheme:
Luciferase
ATP + D-luciferin + O2
oxyluciferin + AMP + PPi + CO2 + light
This bioluminescent reporter gene assay is extremely sensitive and is
especially suitable for quantifying luciferase expression in recombinant
cells. This ultra-sensitive, homogeneous cell-based assay only requires
adding a single reagent to the cells and measuring the light intensity after
a short incubation (>2 minutes). Assays can be performed in tubes, cuvettes
or multi-well plates. All kit components are compatible with culture media
and with all liquid handling systems. With an extended luminescence emission
kinetics (half-life 55 min), the SuperLight luciferase assays are especially
suitable for high-throughput screening in 96-well, 384-well and 1536-well
plates. In addition, the reagent provided in the kits has been formulated
for maximum sensitivity, reproducibility and long shelf-life. Applications
for this kit include gene regulation studies and high-throughput screening
of gene modulators. |
