SUMO E1 activating enzyme is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins.

(SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the N-terminal half of UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thiolester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

Manufacturing: Proteome Resources baculoviral expression in insect cells (Sf9, Spodoptera frugiperda). Form: Solution in 20% glycerol, 150mM NaCl, 5mM DTT, 75mM Tris-HCl, pH 8.0. Concentration: 0.5 µg/µl. Purity: >=98% (SDS-PAGE). Molecular Weight: Aos1 ~ 70 kDa; Uba2 ~ 110 kDa. Storage: -80°C. Avoid freeze/thaw cycles. Shipping: In dry ice. References: Dohmen, R.J., et al.

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