Specialty Proteins
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Ganglioside brain

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Product Code Product Name Price
Specialty Proteins   
304 I270™ AFM Reference €350.00

Product information

Specialty Proteins
I270™ AFM Reference Protein
  I27O™ is a recombinant polyprotein composed of eight (8) repeats of the Ig 27 domain of human titin.  The protein is used as a reference and calibrator for force extension experiments using atomic force microscopes.  Single molecule measurements give up to eight saw tooth force-extension curves with the following specifications:
 
  Parameter Value  
Distance between peaks 24.1 ± 0.34 nm  
Force Peaks 204 ± 26 pN  
Persistence Length 0.39 ± 0.07 nm  
Contour Length (per domain) 28.4 ± 0.3 nm  
 
  I27OÔ is supplied as a ready-to-use solution.  The protein concentration is 0.5-2.0 mg/ml in phosphate buffered saline pH 7.3.  To use, dilute the protein to 100 mg/ml in buffered saline and absorb to freshly prepared gold AFM coverslips.  Secure the coverslips to the retractable stage of the AFM instrument and perform force extension measurements.  The force-extension relationships for the I27O polyprotein are measured using a single axis atomic force microscope.  Single proteins are fully extended by retracting the sample holder away from the AFM tip.  Stretching of the  I27 polyprotein should result in a force-extension curve with peaks that varied randomly in amplitude with an average value of ~200 pN and an average distance between each peak of ~24 nm (see table above).  Between 25% and 50% of the measurements should yield unfolding curves for all eight domains.  Collect data on 20 or more single molecule full-length extensions and calculate the values for the distance between peaks, the magnitude of the force peaks, the persistence length and the contour length for each domain.