siRNA
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Knock-down siRNA Kits
 

Small interfering RNAs (siRNAs) are double stranded RNAs that the mediators of mRNA degradation in the process of RNA interference (RNAi). RNAi has been used as a tool to study gene functions (for a review, see Sharp 2001, Genes Dev 15: 485-490). The siRNA appears that longer dsRNA is processed into 21-23 nt dsRNA (siRNA) by an enzyme, Dicer (Bernstein et al. 2001, Nature 409: 363-366; Knight SW and Bass BL 2001, Science 2: 2269-2271). Spring's Knock-down siRNA Kit provides every things you need for generation of siRNAs from the DNA fragment of target genes. Customers who have the constructs of target genes in vectors containing any two of the promoters, T7, T3, and/or SP6 at the 5'-end and 3'-end of inserted gene can directly generate siRNAs with this kit within one day.

 
Spring's Knock-down siRNA Kit Procedure:
 Spring's Knock-down siRNA Kit has the following advantages:

 
  •  
    		•  Flexible
    This well-designed kit allows you to generate siRNA in your own laboratory.
  •  
    		•  Efficient
    Because this kit starts with a DNA fragment of the target gene, it can generate a mixture of siRNAs that has much more chance to knock-down the target gene than a single or few siRNAs.
  •  
    		•  Cost Effective
    One kit can be used for five genes knock-down experiments and more than 100 tranfections.

     

    Product Type  Sub Type  Catalog No.  Size  Price
     Knock-down siRNA Kit 1 T7 Transcription Kit  KSK-101   5 rxns    € 450.00  
    T7 Trans.Kit
    siRNA Kit
     Knock-down siRNA Kit 2 SP6 Transcription Kit  KSK-102   5 rxns    € 450.00  
    SP6 Trans.Kit
    siRNA Ki
     Knock-down siRNA Kit 3 T3 Transcription Kit  KSK-103   5 rxns    € 450.00  
    T3 Trans.Kit
    siRNA Kit
     Knock-down siRNA Kit 4 T7 Transcription Kit  KSK-104   5 rxns    € 450.00  
    SP6 Trans.Kit
    siRNA Kit
     Knock-down siRNA Kit 5 T7 Transcription Kit  KSK-105   5 rxns    € 450.00  
    T3 Trans.Kit
    siRNA Kit
     Knock-down siRNA Kit 6 SP6 Transcription Kit  KSK-106   5 rxns    € 450.00  
    T3 Trans.Kit
    siRNA Kit
     siRNA Kit Dicer Blend etc.  SRK-101   5 rxns    € 280.00