DESCRIPTION
Chloride is the major extracellular anion in human body fluids. Chloride
plays a key role in maintaining proper water distribution, osmotic pressure
and electrolyte balance in the human body. Low chloride concentrations may
be found with prolonged vomiting, extensive burns, metabolic acidosis,
Addisonia crisis and renal diseases. Elevated chloride concentrations are
associated with dehydration, congestive heart failure, hyperventilation and
urinary obstructions. Determination of chloride in sweat is useful in
diagnosing cystic fibrosis.
Simple, direct and automation-ready procedures for measuring chloride
concentration in biological samples are very popular in clinical studies.
BioAssay Systems' chloride assay kit is designed to measure chloride
directly in biological samples without any pretreatment. The improved Fried
method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored
complex specifically with chloride. The intensity of the color, measured at
610nm, is directly proportional to the chloride concentration in the sample.
The optimized formulation substantially reduces interference by substances
in the raw samples.
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