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TEL: +32 16 58 90 45
Fax :+ 32 16 50 90 45
GENTAUR Europe
tel+32 2 732 5688
fax+32 2 732 4414
Av.
de l' Armée 68
B-1040 BRUSSELS
BELGIUM
GENTAUR France
tel 01 43 25
01 50
fax01 43 25
01 60
9, rue
Lagrange
75005 PARIS
FRANCE
GENTAUR Italy
tel 02 36 00
65 93
fax 02 36 00
65 94
20135 MILANO
ITALY
GENTAUR Germany
tel +49 241
6085 13140
fax +49 241
6085 33033
Forckenbeckstraße 6,
D-52074
Aachen
GERMANY
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Sample Preparation
Optimal sample preparation is
crucial for any proteome analysis. The following parameters define the
quality of each proteomics approach:
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Solubilization
protocol: Our partner Fraunhofer IGB can either apply an in house
optimized SDS solubilization protocol or depending on the project other
solubilization protocols adapted to the range of proteins which needs to
be analyzed. |
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Cell
fractionation: Whenever possible, cell fractions should be used to reduce
the number of total proteins in the 2 compared samples. This increases the
probability of identifying proteome differences. |
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Choice of
electrophoretical separation: Depending on the biological question and on
the number of proteins in the prepared samples, a protein labeling and/or
biochemical fractionation strategy with subsequent 1 D protein gel
electrophoresis or a 2D protein gel electrophoresis strategy might be
selected. |
Ordering information:
Standard Sample Preparation:
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For each protein
extract provided by you, the protein amount will be determined by Bradford
assay and the proteins will be solubilized in an appropriate buffer for
gel loading. |
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Please send your
protein samples on dry ice to BioCat GmbH, Technologiepark, Container, Im
Neuenheimer Feld 581, 69120 Heidelberg. |
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Please enclose a
detailed description how you prepared the samples. |
Cat. No.: SSP01: Standard Sample Preparation for 2D
Gel Electrophoresis; 250 € for 2 samples
Cat. No.: SSP02: Standard Sample Preparation for 1D Gel
Electrophoresis; 125 € for up to 8 samples
Design of Sample Preparation:
If you prefer to supply biological samples rather than protein extracts,
please let us know their source and the biological questions you like to
answer by the proteomics approach. Our partner Fraunhofer IGB will optimize
the proteomics strategy by fractionating the cells or biochemical labeling
if applicable, by choosing the most appropriate solubilization protocol, and
by selecting either 1 dimensional or 2 dimensional electrophoresis as method
of choice. Price depends on the project details.
Please ask for a
quotation.
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