Category	Human tissue array
Cat. No.	CA
Product description	prostate cancer
Data sheet	CA3
	CA3
TNM stage	prostate
Images
	general_information
No. of samples	49
Text	current version: CA3 prostate cancer: 40 cores normal prostate: 9 cores

Price : 5 slides for 600 €

TNM Classification : Prostate carcinoma

T- Primary tumor

TX-primary tumor cannot be assessed

T0- No evidence of primary tumor

T1-Clinically inapparant tumor not palpable or visible by imaging

• T1a-Tumor incidental histologic finding in 5% or less of tissue resected
• T1b-Tumor incidental histologic finding in more 5% or less of tissue resected
• T1c-Tumor identified by needle biospsy

T2-Tumor confined within the prostate

• T2a-Tumor involves one lobe
• T2b-Tumor involves two lobe

T3-Tumor extends through the prostate capsule

• T3a-Extracapsular extension
• T3b-Tumor invades seminal vesicle

T4-Tumor is fixed or invades adjacent structures other than seminal  vesicle: (blader neck,external sphincter, rectum, levator muscle and/or pelvic wall

N-Disntant lymph nodes

NX-Regional lymph nodes cannot be assessed

N0-No regional lymph node metastasis

N1-Regional lymph [h node metastasis]

M-Disntant metastasis

MX-Disntant metastasis cannot be assessed                                                  

M0-No disntant metastasis

M1-Disntant metastasis

• M1a-Non-region lymph node(s)
• M1b-Bone(s)
• M1c-Other site(s)

General Information of the Tissue Array Slide

Applications

• 􀁺 Immunohistochemistry
• 􀁺 In situ hybridization, RNA or DNA
• 􀁺 Fluorescent in situ hybridization (FISH)
• 􀁺 In situ PCR, RNA or DNA
• 􀁺 TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

• 􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
• 􀁺 Tissue array slides are shipped in room temperature.
• 􀁺 If the slides are stored in 4 degree, they are good up to one year. How processed
• 􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
• 􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
• 􀁺 Cleared by xylene, three changes for 1 hour each.
• 􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
• 􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

• 􀁺 Dry slides for 1 hour in a oven at 60 degree.
• 􀁺 Dewax slides in xylene for 4 minutes x 5 times
• 􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
• 􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

• 􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.