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Expression Media |
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Media Optimization Kit™ and APF Media Optimization Kit™ |
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Because the production of a given recombinant protein can
vary significantly depending on the type of medium employed, the Media
Optimization Kit™ and APF Media Optimization Kit™ are used for determining
the best medium formulation for the expression of recombinant proteins in
E. coli.
The APF kit is based on our original Media
Optimization Kit™ except the media are made without animal-derived raw
materials. All the media, except the reference LB Broth, are APF
Certified™.
Either of the Optimization Kits allows the researcher to screen several
specially designed formulations to identify the medium that yields the
highest amount of the target protein. Each kit contains enough media to
prepare 1 liter of each of the six media used by the Contract Services and
Manufacturing Teams at AthenaES to optimize the expression of recombinant
proteins. |
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Standard Kit Components |
APF Kit Components |
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Powder Mixes for 1 liter of: |
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Powder Mixes for 1 liter of: |
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Glucose M9Y |
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Glucose M9Y |
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LB Broth, Miller |
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LB Broth, Miller |
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Hyper Broth™ |
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Hyper Broth™ |
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Turbo Broth™ |
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Turbo Prime
Broth™ |
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Power Broth™ |
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Power Prime
Broth™ |
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Superior Broth™ |
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Superior Prime
Broth™ |
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Applications manual |
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Applications manual |
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100 |
Media Optimization Kit™ |
1 kit |
€50.00 |
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0100-APF |
APF Media Optimization Kit™ |
1 kit |
€50.00 |
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LB Broth - Luria, Lennox, and Miller |
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LB Broth media formulations have been industry standards for
the cultivation of Escherichia coli
since the 1950’s1,2,3.
These media have been widely used in molecular microbiology applications for
the preparation of plasmid DNA and recombinant proteins4,5.
The media are nutrient-rich formulations which provide peptides and
peptones, vitamins, and trace elements. The three formulations differ in
the amount of sodium chloride, thus providing selection of the appropriate
osmotic conditions for the particular bacterial strain and desired culture
conditions. The low salt formulations, Lennox and Luria, are ideal for
cultures requiring salt-sensitive antibiotics such as Zeocin™. |
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Formulas (g/L) |
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Ingredient |
Luria |
Lennox |
Miller |
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Tryptone |
10 |
10 |
10 |
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Yeast Extract |
5 |
5 |
5 |
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NaCl |
0.5 |
5 |
10 |
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|
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101 |
LB Broth (Luria) |
Powder |
500 g |
€27.00 |
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102 |
LB Broth (Lennox) |
Powder |
500 g |
€24.00 |
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|
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113 |
LB Broth (Lennox) |
Liquid, Ready-to-Use |
5 x 500 ml |
€77.50 |
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|
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103 |
LB Broth (Miller) |
Powder |
500 g |
€24.00 |
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114 |
LB Broth (Miller) |
Liquid, Ready-to-Use |
5 x 500 ml |
€77.00 |
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Turbo Broth™ and Turbo
Prime Broth™ |
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Turbo Broth™ and Turbo
Prime Broth™ are proprietary media
formulations used for the cultivation of recombinant strains of
E. coli.
Turbo Prime
Broth™ is made without animal-derived raw materials and is
APF Certified.
These media have been developed to improve the yield of recombinant proteins.
The carbon source is glycerol which is not metabolized into acetic acid. It
has a rich nutrient base which provides amino acids, vitamins, inorganic and
trace minerals at levels higher than those of LB Broth. The medium is
buffered at pH 7.2±0.2 with potassium phosphate to prevent a change in pH
and to provide a source of phosphate. These modifications permit higher
cell yields than can be achieved with LB. For expression of recombinant
proteins, induction should be done at cell densities three times that of LB
Broth. Cell yields are typically 4 to 5 times that of LB Broth in shake
flask cultures depending on the characteristics of the strain. Plasmid copy
numbers are higher, which can also enhance protein expression. This medium
is suitable as the base for fermentation cultures. Biomass yields will
depend on the process employed and the strain. A typical bench-scale
fermentation operated in a batch mode with an air feed can achieve an OD600
of 10 to 12 depending on the strain. |
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104 |
Turbo Broth™ |
Powder |
500 g |
€40.00 |
|
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115 |
|
Liquid, Ready to Use |
5 x 500mL |
€95.00 |
|
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110 |
Turbo Prime
Broth™ |
Powder |
500 g |
€45.00 |
|
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120 |
|
Liquid, Ready to Use |
5 x 500mL |
€105.00 |
|
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Superior Broth™ and Superior
Prime
Broth™ |
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Superior Broth™ and Superior
Prime Broth™
are proprietary medium formulations developed by AthenaES™ for improved
expression of recombinant proteins in E.
coli. Superior
Prime Broth™
is made without animal-derived raw materials and is
APF Certified.
The media are complex, rich formulations which supply a nitrogen source,
vitamins, and moderate glucose levels. The media are buffered at pH 7.2±0.2
to prevent acidification of the medium during metabolism of the glucose.
For expression of recombinant proteins, induction should be done at cell
densities twice that of LB Broth. Cell yields are typically about 3 to 4
times that of LB Broth in shake flask cultures depending on the
characteristics of the strain. Plasmid copy numbers are the highest among
the AthenaES family of media. This medium is an excellent choice for
large-scale plasmid preparations. Biomass yields will depend on the process
employed and the strain. |
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105 |
Superior Broth™ |
Powder |
500 g |
€40.00 |
|
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116 |
|
Liquid, Ready to Use |
5 x500 mL |
€95.00 |
|
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111 |
Superior Prime
Broth™ |
Powder |
500 g |
€45.00 |
|
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121 |
|
Liquid, Ready to Use |
5 x 500 mL |
€105.00 |
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Power Broth™ and Power
Prime Broth™ |
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Power Broth™ and Power
Prime Broth™ are proprietary media
formulations developed by AthenaES™ for improved expression of recombinant
proteins in E. coli.
Power Prime
Broth™ is made without animal-derived raw
materials and is APF Certified.
These media are composed of a complex, rich mixture of amino acids, vitamins,
and a carbon source at higher levels than Terrific Broth. The media are
buffered at pH 6.8±0.2. For expression of recombinant proteins, induction
should be done at cell densities three times that of LB Broth. Cell yields
are typically 3 to 4 times that of LB Broth in shake flask cultures
depending on the characteristics of the strain. Several reports suggest
that Power Broth™ and Power Prime
Broth™ can increase the amount of soluble
protein accumulated for otherwise insoluble proteins. This medium is
suitable as the base for fermentation cultures. Biomass yields will depend
on the process and the strain employed. |
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106 |
Power Broth™ |
Powder |
500 g |
€42.00 |
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117 |
|
Liquid, Ready to Use |
5 x500 mL |
€97.00 |
|
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112 |
Power Prime
Broth™ |
Powder |
500 g |
€45.00 |
|
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122 |
|
Liquid, Ready to Use |
5 x 500 mL |
€105.00 |
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Hyper Broth™ |
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Hyper Broth™ is a proprietary medium formulation developed by
AthenaES™ for improved expression of recombinant proteins in
E. coli.
The medium is a complex, rich formulation which supplies amino acids,
vitamins, and glucose at high levels. It is made without the use of
animal-derived raw materials and is APF
Certified. This medium yields the
highest level of biomass of all AthenaES™ media. The medium is buffered at
pH 7.3±0.2 and has the highest buffering capacity of the Expression Media,
which permits high glucose levels. For expression of recombinant proteins,
induction should be done at cell densities three times that of LB Broth.
Cell yields are typically 4 to 5 times that of LB Broth in shake flask
cultures depending on the characteristics of the strain. Plasmid copy
numbers are higher than for LB, which can facilitate protein expression.
This medium is suitable as the base for fermentation cultures and is
excellent for feed-batch applications. Biomass yields will depend on the
process and the strain employed. Hyper Broth™ powder is supplied with a
separate Glucose Nutrient Mix. |
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107 |
Hyper Broth™ |
Powder |
500 g |
€36.00 |
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118 |
|
Liquid, Ready to Use |
5 x 500mL |
€90.00 |
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Glucose M9Y |
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Glucose M9Y medium, used to cultivate
E. coli, is
a minimal medium formulation supplemented with yeast extract. It is based
on M9 salts6
with a pH of 6.9±0.2 and employs glucose as the carbon source. Alternative
carbon sources may be substituted. Buffering is provided by a
sodium-potassium phosphate system. Ammonium chloride provides a nitrogen
source. Yeast extract is added to improve the protein production capacity
of the medium and to permit cultivation of auxotrophic strains by supplying
a source of amino acids, vitamins and minerals. This differs from the
traditional formulation of glucose M9, which employs casamino acids. Our
experience has shown that yeast extract typically gives higher yields of
recombinant proteins than casamino acids. Glucose M9Y powder is supplied
with a separate Glucose Nutrient Mix. |
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108 |
Glucose M9Y |
Powder |
500 g |
€28.00 |
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119 |
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Liquid, Ready to Use |
5 x 500 mL |
€90.00 |
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AUGMEDIUM™ |
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AUGMEDIUM™ is a medium additive which conditions the cells
prior to induction of recombinant protein expression. This pre-induction
conditioner is designed to increase the level of expression as well as the
fraction of product which accumulates as soluble protein. AUGMEDIUM™ is
specifically intended for use with strains in which the target protein
accumulates as an inclusion body or as an insoluble aggregate. AUGMEDIUM™
is supplied as a powder for preparation of 50x concentrate stock solutions. |
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123 |
AUGMEDIUM™ |
Powder for 100mL stock |
1 packet |
€75.00 |
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124 |
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Powder for 500mL stock |
1 packet |
€300.00 |
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LB*Booster™ |
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LB*Booster™ is a medium additive which increases the level of
production of recombinant proteins. Particularly suitable for improving the
productivity of LB Broth, LB*Booster™ can increase biomass levels by a
factor of up to 5 in any medium formulation. In addition, the supplement
provides essential nutrients selected for their ability to increase the
relative level of recombinant protein accumulation. LB*Booster™ is supplied
as a powder and as a ready-to-use 20x concentrate. LB*Booster™ is
not a
glucose supplement. |
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125 |
LB*Booster™ |
Powder for 100mL stock |
1 packet |
€25.00 |
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126 |
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Powder for 500mL stock |
1 packet |
€100.00 |
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127 |
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20 x Liquid, Ready to Use |
500 mL |
€125.00 |
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Liquid, Ready to Use Products |
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All Expression Media are available as sterile, ready-to-use
liquid media packaged in 500 ml glass bottles for ease of use and storage.
Liquid medium is sold in sets of 5 x 500 ml. |
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HTS Support:media as well as standard
E. coli formulations are available in
large volume containers to support HTS. Custom packaging is available to
meet the specifications for any HTS platform. Please inquire for pricing
and delivery scheduling. |
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Media |
Cat.# |
5 x 500 mL |
10 x 500 mL |
20 x 500 mL |
Custom |
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LB(Lennox) |
113 |
€77.50 |
€150.40 |
€291.40 |
Inquire |
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LB(Miller) |
114 |
€77.50 |
€150.40 |
€291.40 |
Inquire |
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Turbo Broth™ |
115 |
€95.00 |
€184.30 |
€357.20 |
Inquire |
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Turbo Prime
Broth™ |
120 |
€105.00 |
€203.70 |
€394.80 |
Inquire |
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Superior Broth™ |
116 |
€95.00 |
€184.30 |
€357.20 |
Inquire |
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Superior Prime
Broth™ |
121 |
€105.00 |
€203.70 |
€394.80 |
Inquire |
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Power Broth™ |
117 |
€97.00 |
€188.20 |
€364.70 |
Inquire |
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Power Prime
Broth™ |
122 |
€107.00 |
€203.70 |
€394.80 |
Inquire |
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Hyper Broth™ |
118 |
€90.00 |
€174.60 |
€338.40 |
Inquire |
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Glucose M9Y |
119 |
€90.00 |
€174.60 |
€338.40 |
Inquire |
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