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Cell Culture Products
  SFM Screening Kit
  The SFM Screening Kit is intended for researchers seeking to identify the most appropriate SFM formulation for a specific cell type.  The kit contains a 100 ml sample of each of five serum-free media including: BRFF-BMZERO™, BRFF-EPM2™, BRFF-P4-8F™, DMEM/F12, and IMDM along with 25 ml of FNC COATING MIXâ.  Each of the media are complete, ready-to-use.
   
  Storage Store at 4° C  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
415 SFM Screening Kit Liquid, Ready to Use 1 kit €175.00  
  BRFF-BMZERO™
  BRFF-BMZERO™ is a complete serum-free medium designed to grow human breast cells.3  This SFM was originally used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines.  For optimum cell attachment, use FNC COATING MIX®.  
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
           
401 BRFF-BMZERO™ Liquid, Ready to Use 500 mL €60.00  
  BRFF-EPM2™
  BRFF-EPM2™ is a complete serum-free medium designed to grow human epidermal-like cells.4  This SFM formulation was originally optimized for human esophageal epithelial cells.  Explant cultures of human skin in BRFF-EPM2 yields outgrowths of epithelial cells.  This medium is also useful for culturing a number of human cancer cell lines.  For optimum cell attachment, use FNC COATING MIX®.  
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
402 BRFF-EPM2™ Liquid, Ready to Use 500 mL €60.00  
  BRFF-HPC1™
  BRFF-HPC1™ is a complete serum-free medium designed for establishing new cell lines from human prostate tissue.5  Epithelial cell lines, from both benign prostatic hyperplasia and prostatic carcinoma, have been established and maintained in this medium.  This medium contains dihydrotestosterone.  For optimum cell attachment, use FNC COATING MIX®. 
  Storage Store at -70° C, supplied frozen   
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
403 BRFF-HPC1™ Liquid, Ready to Use 500 mL €60.00  
  BRFF-P4-8F™
  BRFF-P4-8F™ was designed to grow the immortalized normal prostatic cell line 267-B1.6  It also supports the growth of certain established human prostatic cancer cell lines such as PC-3.  For optimum cell attachment, use FNC COATING MIX®.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
404 BRFF-P4-8F™ Liquid, Ready to Use 500 mL €60.00  
  DMEM/F12 SFM
  DMEM/F12 is a serum-free medium formulation for general use.  This formulation is a 1:1 blend of DMEM and Ham’s F12 media supplied complete, ready-to-use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types.  Contains no phenol red or antibiotics.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
410 DMEM/F12 Liquid, Ready to Use 500 mL €30.00  
  IMDM SFM
  Iscove’s Modified Dulbecco’s Medium (IMDM) is a standard serum-free medium.  It is supplemented with BPE and EGF and contains L-glutamine and Hepes but no phenol red or antibiotics.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
411 IMDM Liquid, Ready to Use 500 mL €30.00  
  PET
  PET is a cell dissociation mix specially formulated for use with the "Cold Trypsin" method.  When used to detach monolayer cultures of epithelial cells from the growth substratum, this reagent will yield more viable cells from monoloyers grown in serum-free medium.   The solution contains polyvinylpyrrolidone, EGTA and trypsin in a HBS base.
 
Suggested Use:  After rinsing the cell monolayers using HBS, add 3 ml of ice cold PET dissociation reagent.  Do not keep the flask in the incubator.  Incubate at room temperature and monitor the monolayer by microscopy (preferably phase contrast).  When the cells begin to separate, gently remove most of the PET (without removing the cells) and allow the flask to remain at room temperature.  To determine whether the cells are ready to harvest, tap the flask (held in an upright position) firmly by hand.  When the cells float free, re-suspend them in cold HBS supplemented with 5% Fetal Bovine Serum to stop the tryptic action.  Mix well by pipetting the cell suspension, centrifuge and re-suspend the cell pellet in the appropriate complete serum-free medium.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.4-7.6  
Osmolality 270 -300 mOsM  
     
405 PET  Liquid, Ready to Use 100 mL €45.00  
  Freezing Media Pair
  Freezing Media Pair are used to cryopreserve suspensions of viable cells detached from monolayer cultures using the "cold trypsin" method with PET.  Cells which have been cryopreserved in this medium have been shown to have superior viability upon reconstitution.  The Freezing Media Pair has been used extensively to cryopreserve human cells grown in serum-free media.
 
Suggested Use:  To cryopreserve cells, centrifuge the cell suspension.  Re-suspend the cells at 4 x 106 cells/ml in ice-cold Freezing Medium A.  Slowly add an equal volume of ice-cold Freezing Medium B to the cell suspension.  Mix well.  Transfer 1 ml aliquots of the cell suspension to  pre-cooled cryovials.  Freeze slowly using an automatic controlled rate (1°C per minute) freezer to -90°C.  Alternatively, place the cryovials inside a Styrofoam rack and place the rack in a -80°C mechanical freezer for 24 hours.  Transfer the frozen vials to a liquid nitrogen freezer (vapor phase) for permanent storage.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 3 months  
pH 7.7-7.9 for a 1:1 mixture of Media A and Media B  
     
406 Freezing Media Pair Liquid, Ready to Use 50 mL €30.00  
  FNC COATING MIX®
  FNC COATING MIX® is formulated to dramatically increase the attachment of cells to the plastic growth substratum used for culturing mammalian cell lines.  Many fastidious cell types such as human prostate and breast epithelial cells plated in the appropriate serum-free medium will attach and grow more effectively on FNC-coated plastic surfaces.
 
Suggested Use:  To coat the growth surface, add 0.2 ml FNC COATING MIX® per square centimeter of surface area of the culture vessel (for example use 5 ml for a T-25 flask).  The surface should be completely covered with a layer of liquid.  Incubate for 30 seconds and remove the coating mix with a pipet.  Residual FNC COATING MIX® will not adversely affect the viability of the cells to be plated.  Do not return any “used” coating mix to the original container of FNC.  This will compromise the performance of the product.  The coating can be done immediately before seeding the cells or up to one hour beforehand.
  Storage Store at 4° C, FNC COATING MIX® should not be frozen  
Stability at 4° C 2 years  
pH 7.1-7.4  
Osmolality 280 -300 mOsM  
     
407 FNC COATING MIX® Liquid, Ready to Use 50 mL €70.00  
  Hepes Buffered Saline and HBS with Phenol Red
  Hepes Buffered Saline with or without Phenol Red is an isotonic saline buffered with Hepes.  It is used for rinsing cell monolayers prior to cell dissociation using PET or for washing harvested cells.
  Storage Store at 4° C, shipped at room temperature  
Stability at 4° C 2 years  
pH 7.2-7.5  
Osmolality 270 -300 mOsM  
     
408 HBS w/ Phenol Red Liquid, Ready to Use 500 mL €16.00  
409 HBS w/out Phenol Red Liquid, Ready to Use 500 mL €16.00  
  Bovine Pituitary Extract
  Bovine Pituitary Extract prepared from TSE-free animals.  The extracts were prepared from pituitaries harvested from animals in the United States.  The pituitaries were obtained only from USDA inspected facilities where the animals receive pre- and post-mortem inspection and were found free of contagious diseases.  Foot-and-mouth disease, rinderpest, and contagious bovine pleuropneumonia are not present in U.S. herds.  Further, there are no known cases of bovine spongiform encephalopathy (BSE) in the herds from which the pituitaries were obtained.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C not recommended  
Protein Concentration: 9-12 mg/ml by Bradford Assay  
     
413 Bovine Pituitary Extract Liquid, Ready to Use 100 mg €75.00  
414 Bovine Pituitary Extract Liquid, Ready to Use 250 mg €169.00  
  Bovine Pituitary Extract Liquid, Ready to Use bulk inquire  
  CeHR Medium
  CeHR Medium is a defined medium for the cultivation of the nematode Caenorhabditis elegans.  The medium is supplied ready-to-use containing the requisite salts, minerals, vitamins, growth factors, nucleic acids, and amino acids.  Non-fat milk is added and is without antibiotics.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 1-2 weeks  
     
412 CeHR Medium Liquid, Ready to Use 500 mL €120.00