DESCRIPTION
The PiBlueTM Phosphate Assay Kit is based on a proprietary formulation of
the malachite green dye. The PiBlueTM reagent forms a blue colored complex
with free orthophosphate. The rapid color formation from the reaction can be
conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate
reader. The non-radioactive colorimetric assay kits have been optimized to
offer superior sensitivity and prolonged shelf life. The assay is simple and
fast, involving a single addition step for phosphate determination. Assays
can be performed in tubes, cuvettes or multi-well plates. The assays can be
conveniently performed in 96-well plates for high-throughput screening of
enzyme inhibitors.
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