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Product Details: Platelet-derived Growth Factor (PDGF-BB)
human (RECOMBINANT)
Basic Information
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| Product ID: |
GF-070-8 |
| Product Name: |
Platelet-derived Growth Factor
(PDGF-BB) human (RECOMBINANT) |
| Unit: |
500µg |
| Price: |
2.065 Euro |
| Availability: |
Available |
| Category: |
Growth Factors, Cytokines, Receptors
and their Antibodies |
| Subcategory: |
Growth Factors/Cytokines/Enzymes |
Product Details
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| Description: |
Human PDGF-BB homodimer is produced by
genetically engineered yeast, and purified by sequential
chromatography and ionic exchange HPLC. This polypeptide
is composed of two identical polypeptide chains attached
by disulfide bonds. As it has been described in the
natural product, PDGF-BB is partially clipped at
Arg32-Thr33. This cleavage is visible upon reduction of
the polypeptide with a reducing agent. Note: this
clipping does not affect the biological activity.
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| Biological Activity: |
The material is fully active as
determined by its mitogenic activity measured by
stimulation of 3H-thymidine incorporation into human
foreskin fibroblast cells.
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| Formulation: |
Lyophilized powder |
| Purity: |
Over 97% pure by N-terminal amino acid
sequencing, amino acid composition, HPLC analysis, and
SDS gel electrophoresis. |
| Storage Conditions: |
Store at +4°C. Reconstitution is best
in water or buffer near neutral pH. |
| Stability: |
Several months at +4°C. In solution, at
pH 4-7, is stable for 2-3 months at -20°C. |
| Temperature: |
+4°C |
| References: |
1. Ross, R. et al. (1986) Cell 46:155
2. Johnson, A. et al. (1984) EMBO J.3:921
3. Waterfield, M.D. et al. (1983) Nature 303: 35
4. Brake, A.J. et al. (1884) Proc. Natl. Acad. Sci.
(USA) 81:4642 |
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