PDGF-BB hu RECOMBINANT
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Product Details: Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT)

Basic Information

 
Product ID: GF-070-8
Product Name: Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT)
Unit: 500µg
Price: 2.065 Euro
Availability: Available
Category: Growth Factors, Cytokines, Receptors and their Antibodies
Subcategory: Growth Factors/Cytokines/Enzymes

 

Product Details

 
Description: Human PDGF-BB homodimer is produced by genetically engineered yeast, and purified by sequential chromatography and ionic exchange HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent. Note: this clipping does not affect the biological activity.

 
Biological Activity: The material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.

 
Formulation: Lyophilized powder
Purity: Over 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
Storage Conditions: Store at +4°C. Reconstitution is best in water or buffer near neutral pH.
Stability: Several months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C.
Temperature: +4°C
References: 1. Ross, R. et al. (1986) Cell 46:155
2. Johnson, A. et al. (1984) EMBO J.3:921
3. Waterfield, M.D. et al. (1983) Nature 303: 35
4. Brake, A.J. et al. (1884) Proc. Natl. Acad. Sci. (USA) 81:4642