Osteoclast Differentiation Fact.

Catalog No:	OCY002-01 (5ug x 3 vial)
Source:	E.Coli
Molecular Weight:	43.9kDa (GST fusion protein)
Formulation:	Lyophilized from a 0.2mm filtered solution in PBS.
Reconstitution:	Reconstitution in 100ml of ice-cold distilled water on ice.
Storage:	Lyophilized protein is stable for a few weeks at room temperature, but best stored ?20oC. Reconstituted GST-hsRANK ligand should be stored in working aliquots at -20oC.
Purity :	Greater than 98% by SDS-PAGE(CBB staining).

Lane 1 : Marker,
Lane 2: Purified GST-hsRANK Ligand

Biological Activity :

Measured by it's ability to induce osteoclast formation on mouse MDBM
(MCSF-dependent-bone marrow) cells using a concentration of 100 - 150ng/ml.

Note : Avoid repeated freezing/thawing cycles.

Description :

RANK Ligand (receptor activator of NF-Kappa B ligand [RANKL], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family.The mouse RANK Ligand cDNA encodes a type II transmembrane protein. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human RANK Ligand share 85% amino acid identity. The multi-functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Osteoprotegrin, a soluble member of the TNF receptor family which binds RANK Ligand, is a naturally occurring decoy receptor that counterbalances the effects of RANK Ligand