Nycodenz Lympholyte
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METRIZAMIDE AG              04-METR-50gr

135 Euro/ 50 gr

  • DESCRIPTION:

Metrizamide is a tri-iodinated benzamido-derivative of glucose with the systemic name: 2-
(3-acetamido-5-N-methylacetamido-2,4,6-triiodobenzamido)-2-deoxy-D-glucose.
TECHNICAL DATA:
Molecular weight: 789.1
Min. content Metrizamide: *Min. 95%
[a]D (5%), H2O -
Max. O.D. 420nm (60%, H2) 0.300
H2O (Karl Fischer filtration) Max. 5%
Inorganic iodide Max. 20 μg/g
Heavy metals Max. 20 μg/g
Dimethylformamide Max. 5 mg/g
Isopropanol Max. 5 mg/g
*Calculated on dry substance.

  • PRESENTATION: 50 g
  • STABILITY AND STORAGE:
    When using Metrizamide solutions it is important to ensure that the conditions used, do not
    allow the release of iodine from the aromatic ring, since it is possible that the released
    iodine might modify the biological material being fractionated. Iodine atoms absorb in the
    ultra violet region of the spectrum, and when Metrizamide is irradiated with ultraviolet
    light, the iodine atoms are destabilized. Prolonged exposure to sunlight and, to a lesser
    extent, to diffuse daylight and artifical light can also lead to the release of iodine from the
    molecule. However, the extent of this reaction is generally insignificant when working
    with these solutions on a day to day basis. Iodine is also released when Metrizamide
    solutions are heated above 55°C, so this precludes the use of autoclaving for sterilizing
    solutions. Any iodine released from Metrizamide can be neutralized by the addition of
    either sodium thiosulphate or sodium bisulphate though this does introduce extra ions into
    the solution.

CTL lymphocytes can be prepared from splenocytes after removal of plastic adherent cells and centrifugation on a layer of 14.5% (w/v) metrizamide as described (Macatonia et al (1989) J. Exp. Med. 169, 1255-1264). BAN : METRIZAMIDE
CAS : 31112-62-6
DCF : METRIZAMIDE
DCIR : METRIZAMIDE
bordereau : 2437
code expérimentation : Win-39103
dci : métrizamide
rINN : METRIZAMIDE
sel ou dérivé : METRIZOATE CALCIQUE
sel ou dérivé : METRIZOATE DE MAGNESIUM
sel ou dérivé : METRIZOATE DE MEGLUMINE
sel ou dérivé : METRIZOATE SODIQUE