> Whatman FTA cards

FTA Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA Cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms. Try FTA, and you'll soon find it's an indispensable part of your DNA toolbox. US Patent Nos. 5496562, 5756126, 5807527, 5972386, 5985327 and other patents pending

Features and Benefits

Electron micrograph showing DNA entrapped within the FTA matrix (magnification x 10,000).

Capture nucleic acid in one easy step

• Captured nucleic acid is ready for downstream applications in less than 30 minutes
• Nucleic acids collected on FTA Cards are stable for years at room temperature
• FTA Cards are stored at room temperature before and after sample application, reducing the need for laboratory freezers
• Suitable for virtually any cell type
• Indicating FTA Cards change colour upon sample application to facilitate handling of colourless samples
• FTA Cards are available in a variety of configurations to meet application requirements
• Custom configurations are available on request

pplications

• Forensics
• Transgenics
• Transfusion Medicine
• Plasmid Screening
• Food & Agriculture Testing
• Drug Discovery
• Genomics
• STR Analysis
• Animal Identification
• Diagnostics
• Pharmacogenomics
• Molecular Biology

Capture Nucleic Acids in One Easy Step

Simply apply your sample to the FTA Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibres of the card. The nucleic acids remain immobilized and are stabilized for transport, immediate processing or long-term room temperature storage.

Since captured nucleic acids are stabilized, FTA Cards facilitate sample collection in remote locations and simplify sample transport. For example, you can collect samples deep in a rain forest without worrying about immediate refrigeration. Ship your samples back to the lab without expensive special handling or dry ice, and process at your convenience.

Indicating FTA Cards are recommended for colourless samples. These FTA Cards change from pink to white when sample is applied, verifying the location of the sample.

FTA Cards Used With Virtually Any Sample Type

• Blood
• Cultured Cells
• Buccal Cells
• Plant Material
• Bacteria
• Plasmids
• Microorganisms
• Solid Tissue
• Viral Particles
• M13 Plaques
• ...and more

Captured Nucleic Acid is Ready for Downstream Applications in Less than 30 Minutes
Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA Card, wash with FTA Purification Reagent and rinse with TE-1 buffer. DNA on the washed punch is ready to use in applications such as PCR, RFLP analysis and RT-PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.

Store Nucleic Acids at Room Temperature for Years
Genomic DNA stored on FTA Cards at room temperature for over 14 years (and counting) has been successfully amplified by PCR. In contrast, genomic DNA stored at room temperature on non-FTA Cards for over six months did not amplify.

Sample integrity is optimized when FTA Cards are stored in a Multi-Barrier Pouch with a Desiccant Packet.

FTA Cards offer a compact room-temperature storage system that reduces the need for precious freezer space.

FTA Classic Card
Four sample areas for application of up to 500 µL whole blood or 100 µL plant homogenate per card. Convenient for multiple applications of the same specimen or collection of multiple animal or plant samples on one card. Different samples can be processed independently.

Indicating FTA Classic Card
Same as FTA Classic Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with colourless samples such as buccal or culture cells.

FTA Mini Card
Two sample areas for application of up to 250 µL whole blood or 50 µL plant homogenate per card. Convenient for protocols that require different locations for testing and archiving samples. Different samples can be processed independently.

Indicating FTA Mini Card
Same as FTA Mini Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with clear samples such as buccal or culture cells.

FTA Micro Card
One sample area for application of up to 125 µL whole blood or 25 µL plant homogenate per card. Recommended when only one sample is needed.

Indicating FTA Micro Card
Same as FTA Micro Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with clear samples such as buccal or culture cells.

FTA Gene Card
An FTA card enclosed in a rigid card frame. Three sample areas for application of up to 225 µL whole blood or 30 µL plant homogenate per card. Can be utilized in many automatic dispensing/pipetting systems when used with the FTA Gene Card Tray (WB100030).

PlantSaver™ FTA Card
Plant friendly FTA Card, in a Classic Card format. Features a laminated flap that allows you to vigorously pound the plant sample into the FTA matrix without damaging the FTA Card.

FTA Kit
Includes: 25 FTA Micro Cards, 2 vials purification reagent (25 mL), 2 Harris Uni-Core Punches with cutting mat, instructions.

FTA Starter Pack
Includes: 1 FTA Classic Card, 1 FTA Mini Card, 1 FTA Micro Card, 1 Indicating FTA Mini Card, 1 FTA Indicating Micro Card, 2 foam-tipped applicator swabs, 1 multi-barrier pouch and desiccant, 1 vial purification reagent (25 mL), 2 Harris Uni-Core Punches with cutting mat, instructions.

Blood Stain Card
Contains non-FTA absorbent filter paper for the collection and transport of blood and body fluids. Intended for short-term handling of specimens and for protein or metabolite studies. For long-term storage of nucleic acids, Whatman FTA Cards are recommended. There are four sample areas for application of up to 500 µL whole blood per card. Convenient for multiple applications of the same specimens or collection of multiple specimens on one card. Different samples can be processed independently.

> GenSpin genomic and plant DNA purification kit

GenSpin™ Genomic DNA Purification Kit
The GenSpin Purification Kit is designed to purify high quality, PCR-ready, single stranded DNA in solution from whole blood and cultured cells in as little as 25 minutes.

This simple protocol uses a single micro centrifuge tube and small sample volume (5-50 µL) to produce high quality DNA for amplification by PCR. DNA for up to 80 amplification reactions can be obtained from a 50 µL fresh or anticoagulant-treated blood sample.

GenSpin incorporates patented FTA technology, which lyses cell and nuclear membranes on contact. The DNA is reversibly entrapped within this type of FTA filter matrix and can be stored for weeks at room temperature prior to purification. Cellular debris and proteins are removed by washing with GenSpin Buffer and TE-1 Buffer using a centrifuge. The purified DNA is released from this specialized filter matrix by heat elution and is ready for immediate PCR amplification.

Features and Benefits

• Simple, single tube protocol. Purify single stranded DNA from whole blood and cultured cells in less than 25 minutes
• FTA technology lyses cells, denatures proteins and inactivates viral contaminants. Allows room temperature storage of DNA and safe sample transport and handling prior to purification
• High quality DNA. Enables full length PCR amplification
• Highly efficient method. Enables purification of sufficient DNA for up to 80 amplification reactions from a 50 µL blood sample
• No precipitation steps. DNA is ready for immediate analysis

GenSpin™ Plant DNA Purification Kit

The GenSpin Plant DNA Purification Kit is designed for the rapid preparation of double-stranded DNA in solution from small quantities of plant material for PCR analysis. Using a single microcentrifuge tube, this simple protocol enables the recovery of DNA for more than 50 amplification reactions from just 10 mg of plant tissue. The small sample capability is ideally suited for rapid analysis studies such as identification of genetically-modified plants and cultivar screening.

Plant material is homogenized at room temperature and applied to the GenSpin Filter Basket, which incorporates FTA technology to immediately stabilize the DNA at room temperature. Nucleases are inactivated and the DNA is protected from UV and environmental damage. The immobilized DNA is entrapped in the fibres of the matrix and can either be purified immediately or stored at room temperature for more than 4 weeks. The filter is washed with two reagents to remove contaminants that would inhibit PCR. The DNA is then eluted from the filter by heating and collected by centrifugation.

Features and Benefits

• Simple, single tube protocol. Eliminates need for organic solvents, liquid nitrogen and time-consuming precipitation steps
• Fast purification of DNA. Purify DNA in less than 30 minutes for quick sample screening. Up to 50 amplifications from only 10 mg of plant material
• PCR-ready double-stranded DNA. Reliable amplification of DNA for a wide range of applications including cultivar screening and ID of genetically modified plants
• FTA technology protects DNA from degradation. Enables room temperature storage for weeks