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 Normal organs and cancers
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Category Human tissue array
   Cat. No. BC
   Product description Normal organs and cancers
   Data sheet BC5
BC5
   Images
  general_information
   No. of samples 59
   Text
Current version : BC5
No. of normal cores : 30 cores
No. of cancer cores : 29 cores

Price : 5 slides for 600 €

General Information of the Tissue Array Slide

Applications

bullet􀁺 Immunohistochemistry
bullet􀁺 In situ hybridization, RNA or DNA
bullet􀁺 Fluorescent in situ hybridization (FISH)
bullet􀁺 In situ PCR, RNA or DNA
bullet􀁺 TUNEL for apoptosis 

Storage and stability

bullet􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
bullet􀁺 Tissue array slides are shipped in room temperature.
bullet􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

bullet􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
bullet􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
bullet􀁺 Cleared by xylene, three changes for 1 hour each.
bullet􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
bullet􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

bullet􀁺 Dry slides for 1 hour in a oven at 60 degree.
bullet􀁺 Dewax slides in xylene for 4 minutes x 5 times
bullet􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
bullet􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

bullet􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area
1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60

 

BC5   Normal organs and cancers
         
  Age Sex organ Diagnosis
1 63 F skin normal
2 44 F breast normal
3 53 F spleen normal
4 55 M lymph node normal
5 57 M skeletal muscle normal
6 72 F lung normal
7 69 M salivary gland, sublingual normal
8 47 M liver normal
9 65 M gallbladder normal
10 73 F pancreas normal
11 24 M tonsil normal
12 56 M esophagus normal
13 31 M stomach, antrum normal
14 59 M stomach, fundus normal
15 52 F small bowel normal
16 60 M colon normal
17 54 M rectum normal
18 48 M kidney, cortex normal
19 48 M kidney, medulla normal
20 32 M bladder normal
21 32 M prostate normal
22 81 M testis normal
23 39 F uterine cervix normal
24 49 F endometrium normal, proliferative
25 42 F myometrium normal
26 35 F placenta normal
27 48 M adrenal normal
28 39 M thyroid normal
29 0 M brain normal
30 12 M cerebellum normal
31 57 M skin squamous cell carcinoma
32 64 F skin malignant melanoma
33 38 M subcutaneous tissue liposarcoma, myxoid
34 34 F breast ductal carcinoma in situ
35 43 F breast Infiltrating duct carcinoma
36 33 F lymph node Hodgkin's disease
37 53 F bone osteosarcoma
38 60 M lung adenocarcinoma