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TEL: +32 16 58 90 45
Fax :+ 32 16 50 90 45
GENTAUR Europe
tel+32 2 732 5688
fax+32 2 732 4414
Av.
de l' Armée 68
B-1040 BRUSSELS
BELGIUM
GENTAUR France
tel 01 43 25
01 50
fax01 43 25
01 60
9, rue
Lagrange
75005 PARIS
FRANCE
GENTAUR Italy
tel 02 36 00
65 93
fax 02 36 00
65 94
20135 MILANO
ITALY
GENTAUR Germany
tel +49 241
6085 13140
fax +49 241
6085 33033
Forckenbeckstraße 6,
D-52074
Aachen
GERMANY
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Price : 5 slides for 600 €
General Information of the Tissue Array Slide Applications
 | Immunohistochemistry |
| In situ hybridization, RNA or DNA |
| Fluorescent in situ hybridization (FISH) |
| In situ PCR, RNA or DNA |
| TUNEL for apoptosis |
Storage and stability
| Individual slide is paraffin-coated and put in an air-tight pack with
inert gas. |
| Tissue array slides are shipped in room temperature. If the slides
are stored in 4 degree, they are good up to one year. |
How processed
| Tissues were initially fixed with formalin except for some of the
animal tissues. |
| Then, dehydrated with gradient ethanol; typically 1 hour each
progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times. |
| Cleared by xylene, three changes for 1 hour each. |
| Infiltrated with 60°C paraffin, three changes for 1 hour each
|
| Microtoming in 4 µm thickness and put on silane coating slide from
Muto Pure Chemicals (Japan) |
Before use
| Dry slides for 1 hour in a oven at 60 degree. |
| Dewax slides in xylene for 4 minutes x 5 times |
| Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times
each. |
| Immerse slides in tap water for 5 minutes |
Slide orientation
| In most of the slides with 59 or 60 cores, the orientation is as
below unless indicated otherwise. #60 location is usually filled with carbon
for orientation. |
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