Normal organs
Home Up Normal organs Evaluation slide (TEST SLIDE) Various cancers Normal organs and cancers prostate cancer Breast: cancer-metastasis-normal lung cancer Lung: cancer-metastasis-normal Normal lung tissue colon and rectum cancer colorectal: cancer-metastasis-normal Normal colon and rectum tissue larynx and pharynx cancer Ovary cancer Kidney cancer Stomach cancer Normal stomach tissue Esophagus cancer Liver cancer Liver: cancer-metastasis-normal Non-neoplastic liver tissue Thyroid cancer Lipomatous tumors Skin cancer Normal endometrium

 


 
   Category Human tissue array
   Cat. No. AA
   Product description Normal organs
   Data sheet AA9
AA9
   Images
  general_information
   No. of samples 59
   Text
Current version: AA9
Various normal organs: 59 cores

 

 

 

Price :  5 slides for 600 €

 

General Information of the Tissue Array Slide Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis

Storage and stability

  • 􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • 􀁺 Tissue array slides are shipped in room temperature. 􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

  • 􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
  • 􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • 􀁺 Cleared by xylene, three changes for 1 hour each.
  • 􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
  • 􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • 􀁺 Dry slides for 1 hour in a oven at 60 degree.
  • 􀁺 Dewax slides in xylene for 4 minutes x 5 times
  • 􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • 􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

  • 􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

 

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