Normal lung tissue
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   Category Human tissue array
   Cat. No. CCA
   Product description Lung: cancer-metastasis-normal
   Data sheet CCA3
CCA3
   
   Images
  general_information
   No. of samples 59
   Text
(Old Cat. No: CC3)
current version : CCA3
lung cancer : 40 cores
metastatic cancer : 10 cores
normal : 9 cores

Price : 5 slide for 600 €

CCA3  Human, lung cancer
               
  Age  Sex Organ Diagnosis TNM Stage Months*
1 63 M lung squamous cell carcinoma T2N0M0 ⅠB .
2 63 M lung squamous cell carcinoma T2N0M0 ⅠB .
3 50 M lung squamous cell carcinoma T2N1M0 ⅡB .
4 67 M lung squamous cell carcinoma T2N1M0 ⅡB .
5 54 M lung squamous cell carcinoma T2N0M0 ⅠB .
6 39 M lung squamous cell carcinoma T4N1M0 ⅢB .
7 71 M lung squamous cell carcinoma T2N0M0 ⅠB .
8 67 M lung squamous cell carcinoma T2N1M0 ⅡB .
9 65 M lung squamous cell carcinoma T2N1M0 ⅡA .
10 47 M lung adenocarcinoma T2N1M0 ⅡA .
11 38 M lung adenocarcinoma T2N0M0 ⅠB .
12 48 F lung adenocarcinoma T1N0M0 ⅠA .
13 67 M lung adenocarcinoma T2N0M0 ⅠB .
14 55 M lung bronchioloalveolar carcinoma T1N0M0 ⅠA .
15 69 F lung bronchioloalveolar carcinoma T2N0M0 ⅠB .
16 52 F lung bronchioloalveolar carcinoma T2N0M0 ⅠB .
17 34 0 lung bronchioloalveolar carcinoma T2N0M0 ⅠB .
18 47 M lung adenosquamous carcinoma T2N2M0 ⅢA .
19 60 M lung adenosquamous carcinoma T2N2M0 ⅢA .
20 60 F lung small cell carcinoma T2N0M0 ⅠB .
21 65 M lung small cell carcinoma T2N0M0 ⅠB .
22 60 M lung combined small cell + adenocarcinoma T2N1M0 ⅡB .
23 43 M lung large cell carcinoma T2N1M0 ⅡB .
24 56 M lung large cell carcinoma T2N1M0 ⅡB .
25 63 M lung large cell carcinoma T2N0M0 ⅠB .
26 37 M lung mucoepidermoid carcinoma T1N0M0 ⅠA .
27 40 F lung mucoepidermoid carcinoma T2N1M1 Ⅳ .
28 26 F lung mucoepidermoid carcinoma T2N0M0 ⅠB .
29 54 F lung adenoid cystic carcinoma T4N1M0 ⅢB .
30 37 M Pleura malignant mesothelioma T2N0M0 Ⅰ .
31 45 M lung squamous cell carcinoma T2N3M0 ⅢB .
32 59 M lung squamous cell carcinoma T2N2M0 ⅢA .
33 62 M lung squamous cell carcinoma T2N2M0 ⅢA .
34 39 M lung squamous cell carcinoma T4N1M0 ⅢB .
35 58 M lung adenocarcinoma T3N0M0 ⅡB .
36 54 M lung adenocarcinoma T2N1M0 ⅡA .
37 62 F lung adenocarcinoma T2N2M0 ⅢA .
38 72 M lung mucinous adenocarcinoma T2N2M0 ⅢA .
39 67 F lung adenosquamous cell carcinoma T2N0M0 ⅢA .
40 56 M lung mucoepidermoid carcinoma T2N0M0 ⅠB .
41 45 M lymph node metastatic carcinoma from #31 0 . 0
42 59 M lymph node metastatic carcinoma from #32 0 . 0
43 62 M lymph node metastatic carcinoma from #33 0 . 0
44 39 M lymph node matastatic carcinoma from #34 0 . 0
45 66 M bone metastatic carcinoma from #35 0 . 17
46 54 M lymph node metastatic carcinoma from #36 0 . 0
47 62 F lymph node metastatic carcinoma from #37 0 . 0
48 72 M lymph node metastatic carcinoma from #38 0 . 0
49 76 F femur metastatic carcinoma from #39 0 . 17
50 63 M soft tissue metastatic carcinoma from #40 0 . 11
51 67 M lung normal lung (adjacent to cancer, match of #8) 0 . .
52 58 M lung normal lung (adjacent to cancer, match of #12) 0 . .
53 34 0 lung normal lung (adjacent to cancer, match of #17) 0 . .
54 60 F lung normal lung (adjacent to cancer, match of #20) 0 . .
55 59 M lung normal lung (adjacent to cancer, match of #32) 0 . .
56 63 M lung normal lung(apart from cancer, match of #1) 0 . .
57 55 M lung normal lung (apart from cancer, match of #14) 0 . .
58 43 M lung normal lung (apart from cancer, match of #23) 0 . .
59 58 M lung normal lung (apart from cancer, match of #35) 0 . .
60 carbon            
               
*: interval between primary and metastatic cancer        
TNM Classification : Lung carcinoma (AJCC 6th Edition)        

General Information of the Tissue Array Slide :

Applications

  • 􀁺 Immunohistochemistry
  • 􀁺 In situ hybridization, RNA or DNA
  • 􀁺 Fluorescent in situ hybridization (FISH)
  • 􀁺 In situ PCR, RNA or DNA
  • 􀁺 TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • 􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • 􀁺 Tissue array slides are shipped in room temperature.
  • 􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

  • 􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
  • 􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • 􀁺 Cleared by xylene, three changes for 1 hour each.
  • 􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
  • 􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • 􀁺 Dry slides for 1 hour in a oven at 60 degree.
  • 􀁺 Dewax slides in xylene for 4 minutes x 5 times
  • 􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • 􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

  • 􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

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