Na+/ H+ exchanger regulatory factor (NHERF) is a PDZ domain containing adaptor protein known to bind to various receptors, channels, cytoskeletal elements and cytoplasmic signaling proteins. A 358aa mainly expressed in kidney, liver and pancrease by NHERF gene (chrm 17). and is represented in 2 isoforms (NHERF1 and NHERF2). NHERF1 protein contains 2 tandem PDZ domains of approximately 90aa and a C-terminal sequence that binds several members of the ERM (ezrin-radixin-moesin) family of membrane cytoskeletal adapters. The protein plays an important role in regulation of NHE3, turnover of G-protein coupled receptors, platelet derived growth factor receptor and ion transporters such as CFTR, Na/Pi cotransporter, NaHCO3 cotransporter and Trp channels.

NHERF1 overexpression in cancers and mutations in NHERF1 targets, Merlin, the product of tumor suppressor gene, also expressed in 10 isoforms, Merlin is involved in pathogenesis of benign tumors of the human nervous system, Merlin is named for its striking similarity with ERM family which thought to link cytoskeletal components with proteins in the cell membrane It has a binding partner called Syntenin, which is an adapter protein that couples transmembrane proteoglycans to cytoskeletal components.

Merlin, Isoforms 1 and 2 are predominant, isoforms 4, 5 and 6 are expressed moderately, and isoform 8 is found at low frequency. Isoforms 7, 9 and 10 are not expressed in adult tissues, isoforms 1& 2 are 595aa and 590aa each in human, from same gene locus on chromosome 22q12, with approximate mol. wt of 70kD, they are structurally related to moesin, ezrin and redixin but not functionally. The Merlin isoforms are widely expressed in heart, lungs, skeletal muscle and spleen. Since being a membrane stabilizing protein, defects may lead to neurofibromatosis 2 a genetic disorder characterized by bilateral vestibular schwannomas. Affected individuals generally develop symptoms of eighth-nerve dysfunction in early adulthood, including deafness and balance disorder.

Syntenin, a 300aa protein in mouse and 298aa each in rat and human, with a mol wt of 32kD. Functions as an adapter protein, play a role in vesicular trafficking and required for the targeting of TGFA to the cell surface in the early secretory pathway. Expressed mainly in fetal kidney, lung, liver and brain, in adult highest expression in heart and placenta. It localizes adheren junctions, focal adhesions and endoplasmic reticulum.

Rb=rabbit; m=mouse; r=rat; h=human; b=bovine; c=chicken; d=dog; ~CT or ~NT=near C or N-terminus. EC=Extracellular; CL=Cytoplasmic loop

* Expected antibody crossreactivity information is mostly based upon high (>70%) sequence conservation of antigenic/control peptides in various species. When antibody crossreactivity has actually been experimentally confirmed in various species, it will be mentioned in the appropriate data sheets.

"Neat Antisera or antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" IgG may be more suitable for immunohistochemical (IHC) applications and to reduce background in most immunological applications including ELISA and Western.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody blocking studies to establish antibody specificity.
Western blot +ve protein controls, where available, are semi-pure, pure or recombinant proteins that are formulated in SDS-PAGE sample buffer. They are recommended to be used for Western (load 10 ul/lane) for visualization with antibodies.