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Multiwell accs.

UNIFILTER® Filtration Microplates



> UNIFILTER Microplate

The patented Whatman UNIFILTER microplates with filter-bottom wells are convenient and ready to use. Available in 24, 96 and 384 well formats, UNIFILTER microplates offer a choice of filter media to meet exact application requirements.

The unique drip director design of Whatman UNIFILTER microplates ensures precise collection of the filtrate to allow for further processing and analysis. UNIFILTER microplates are available in a range of well volumes from 100 µL to 10 mL.

Features and Benefits

	No crosstalk. Patented integral filter design prevents well-to-well cross contamination
	Economical to use. Wide range of well volume options ensures efficient use of materials
	Better control. Choice of filter media allows control of the flow rates and retention characteristics
	Versatile. A broad range of filtration media is available including glass fibre, polypropylene, cellulose nitrate, cellulose acetate, nylon and ion exchange cellulose

Typical Data - UNIFILTER
Filter Media	Flow Rate*	Protein Binding	Hydrophilic	Solvent Resistance	Physical Strength	Thermal Resistance °C	General Comments
Cellulose Nitrate (CN)	4	High	Yes	Poor	Brittle	<125	Highly adsorptive membrane (CN) typically used for DNA/RNA/ protein hybridization, also for ELISA and RIA-based assays.
Cellulose Acetate (CA)	3	Low	Yes	Poor	Moderate	<120	Typically used for low protein binding applications, good wet strength. General purpose microbiological filter.
Polypropylene (PP)	2	Negligible	No	Very Good	Good	<80	Typically used for prefiltration. Sensitive to gamma sterilization. Very low extractables, chemically inert.
Polyvinylidene fluoride (PVDF) Hydrophilic**	4	Low	Yes	Good	Good	<135	Low protein binding, good chemical resistance.
Glass Microfiber (GF)	5	Moderate	Yes	Very Good	Poor	High	Wide range available. Typically used as absorptive or adsorptive wicking media and prefilters. Excellent particle retention and resistance to clogging. Used for DNA binding.

96 Well 800 µL UNIFILTER microplate



> 96 Well 800 µL UNIFILTER microplate

96 Well 800 µL UNIFILTER microplate
The 800µL UNIFILTER is the microplate most typically used in purifications, isolations and separation of biomolecules, particularly DNA.

The microplate has a well volume of 800µL which is ideal for standard DNA plasmid miniprep chemistries. The choice of short or long drip directors is application specific. The UNIFILTER 800µL is constructed from rigid high grade polystyrene.

96 Well 800 µL UNIFILTER® Microplate
Catalogue Number	Well Format	Well Volume (µL)	Plate Material	Drip Director	Filter Media	Quantity/Case
7700-1801	96	800	Clear Polystyrene	Short	Whatman GF/C	25
7700-1804	96	800	Clear Polystyrene	Short	25–30 µm melt blown polypropylene	25
7700-1806	96	800	Clear Polystyrene	Short	0.45 µm hydrophilic PVDF	25
7700-1808	96	800	Clear Polystyrene	Short	0.45 µm Cellulose Acetate	25
7700-1818	96	800	Clear Polystyrene	Short	5–7 µm melt blown polypropylene	25
7700-2801	96	800	Clear Polystyrene	Long	Whatman GF/C	25
7700-2803	96	800	Clear Polystyrene	Long	Whatman GF/B	25
7700-2804	96	800	Clear Polystyrene	Long	25–30 µm melt blown polypropylene	25
7700-2805	96	800	Clear Polystyrene	Long	0.45 µm PP membrane	25
7700-2806	96	800	Clear Polystyrene	Long	0.45 µm hydrophilic PVDF	25
7700-2808	96	800	Clear Polystyrene	Long	0.45 µm Cellulose Acetate	25
7700-2809	96	800	Clear Polystyrene	Long	0.45 µm Nylon Positive	25
7700-2810	96	800	Clear Polystyrene	Long	DNA binding plate	25
7700-2811	96	800	Clear Polystyrene	Long	Whatman GF/D	25
7700-2817	96	800	Clear Polystyrene	Long	10–12 µm melt blown polypropylene	25
7720-2830	96	800	Clear Polystyrene	Long	Lysate clarification plate	25
7700-2828	96	800	Clear Polystyrene	Long	Whatman oleophobic PKP	10
7770-0062	96	800	Clear Polystyrene	Long	25 µm melt blown polypropylene over 0.45 µm PP membrane	25

Multiwell accessories



> BugStopper microplate capmat

BugStopper™ microplate capmat
Whatman BugStopper™ Capmats provide a simple and reliable method for venting cultures being grown in a 24 well microplate. This reusable sterile closure, which is produced using chemically resistant biosafe silicone rubber, incorporates hydrophobic microfilters which provide an ideal vent for each well.
More efficient than plastic lids, test comparisons confirm that BugStopper Capmats improve cell growth and significantly reduce evaporation. The silicone rubber portion of the capmat reseals after puncture, thus keeping cell cultures sterile during inoculation or aspiration.

Sterile Venting Closures for Microplate Cultures

	More efficient than plastic lids. Perfect for extended growth of slow growing bacteria and fungi
	Positive seal for every well. Significantly reduces evaporation rate and eliminates well-to-well cross-contamination
	Autoclave and re-use. Cost-effective; repeated autoclave cycles do not affect gas exchange or retention capabilities
	Rated 99.9% efficient for bacteria and viruses. Restricts microorganisms while allowing H₂O and CO₂ to pass through the membrane
	Prevents aerosol formation. Suitable for growth of infectious microorganisms

Ordering Information
Catalogue Number	Well Format	Item	Material	Quantity/Case
7704-0014	24	BugStopper Venting Capmat for 10 mL Microplates	Silicone Rubber	5
7701-5102	24	Growth Plate, 10 mL, round-bottom	Polypropylene	25

membrane filters



membrane filters

Whatman brings to the laboratory user a range of membrane filters whose advanced technical specifications makes them today's preferred choice for a wide range of applications. The membrane filters offer accurately controlled pore size distribution and higher strength and flexibility which ensure reproducibility and consistency. The Whatman membrane filter range includes pore sizes (from 0.02 to 5µm) with a wide selection of membrane filters. Sterile and autoclave packs are available for specialized applications. Colored and gridded types are also available.

To access the range of Whatman Membrane Filters, please click on the links on the navigation box to the right.

Anodisc inorganic membranes



> Anodisc inorganic membranes

The Anopore® inorganic membrane is ideal for a wide range of laboratory filtration applications. This unique material has a precise, non-deformable honeycomb pore structure with no lateral crossovers between individual pores.that filters at precisely the stated cutoff, allowing no larger sized particles to pass through the membrane. The Anopore inorganic membrane is composed of a high purity alumina matrix that is manufactured electrochemically. The membrane also exhibits low protein binding, has minimal autofluorescence, is non-toxic and supports cellular growth.

The precise pore structure and narrow pore size distribution of the Anopore membrane ensure a high level of particle removal efficiency. Micro-organisms and particulate material are captured on the surface of the membrane for subsequent analysis by light or electron microscopy. When wet, the membrane is virtually transparent, which means that retained particles do not need to be transferred to another surface before microscopic examination.

> Anodisc pore structure



> Anodisc pore structure

The membrane is hydrophilic and is compatible with most solvents and aqueous material. No monomers, plasticizers, adhesives, surfactants or wetting agents are used in the manufacturing process, which eliminates sample contamination and ensures low protein binding and minimal loss of sample.

The Anopore membrane is supplied in the form of Anodisc membrane filters. The membrane is peripherally bonded to an annular polypropylene ring (except the 13 mm diameter disc) for ease of handling and is suitable for both vacuum and pressure filtration.

Anopore is available in 3 nominal pore sizes: 0.02µm, 0.1µm and 0.2µm and in 3 disposable syringe filter housings: 13mm, 25mm and 47mm.

Features and Benefits

	High pore density and narrow pore size distribution make it an extremely precise membrane
	Wide solvent compatibility reduces the need to stock a variety of membranes in the laboratory
	No additives used in the manufacturing process ensures minimal extractables and no sample contamination
	Extremely low protein binding minimizes sample loss
	Virtually transparent when wet making it ideal for microscopy studies

Applications

	HPLC mobile phase filtration and degassing
	Ultra cleaning of solvents
	Gravimetric analysis
	Liposome extrusion
	Scanning electron microscopy studies
	Bacterial analysis by epifluorescence light microscopy
	Micrometer and nanometer filtration
	Metal nanorods formation

Typical Data
	Anodisc 13	Anodisc 25	Anodisc 47
Average Membrane Thickness	60 µm	60 µm	60 µm
Membrane Diameter	13 mm	21 mm	43 mm
Membrane Type	Anopore
	aluminum oxide
Support Ring Material	None	Polypropylene	Polypropylene
Construction Process	None	Thermal weld	Thermal weld
Protein Adsorption	Low	Low	Low
Burst Strength	65–110 psi	65–110 psi	65–110 psi
Maximum Service Temperature	400°C	400°C	400°C
Porosity	25-50%	25-50%	25-50%
Autoclavable	Yes	No	No
Refractive Index	1.6	1.6	1.6

Ordering Information - Diameter (mm)
Diameter (mm)	Pore Size (µm)	Membrane	Catalogue Number	Hydrophilic	Protein Binding	Solvent Resistance	Quantity/Pack
13	Anodisc 13*	0.02	6809-7003	Yes	Low	Very Good	100
13	Anodisc 13*	0.1	6809-7013	Yes	Low	Very Good	100
13	Anodisc 13*	0.2	6809-7023	Yes	Low	Very Good	100
25	Anodisc 25	0.02	6809-6002	Yes	Low	Very Good	50
25	Anodisc 25	0.1	6809-6012	Yes	Low	Very Good	50
25	Anodisc 25	0.2	6809-6022	Yes	Low	Very Good	50
47	Anodisc 47	0.02	6809-5002	Yes	Low	Very Good	50
47	Anodisc 47	0.1	6809-5012	Yes	Low	Very Good	50
47	Anodisc 47	0.2	6809-5022	Yes	Low	Very Good	50

Application specific plates



> ELISA UNIFILTER

ELISA UNIFILTER®
The Whatman ELISA plate allows researchers to use the binding characteristics of nitrocellulose in the standard SBS 8 x 12 multiwell format. It is also suitable for other types of ligand binding applications such as hybridoma supernatant screening. Solutions are easily vacuumed to waste using a vacuum manifold.
Chemiluminescent assays can be read in the plate. If the ELISA product is eluted then centrifugation is preferred. This microplate is compatible with automated systems.

Ordering Information - ELISA UNIFILTER®
Catalogue Number	Well Format	Well Volume	Plate Material	Filter Media	Well Bottom	Quantity/Case
7700-3307	96	350 µL	White Polystyrene	0.45µm CN *	Filter	50
7701-1350	96	300 µL	Clear Polystyrene	N/A	Flat	50
7701-5200	96	2 mL	Natural Polypropylene	N/A	Round	25
7705-0107	UniVac™ Vacuum to Collect Manifold, acrylic					1
7704-0001	Clear Polyester ThinCold Sealing Film Adhesive Backing					100
* CN = Cellulose nitrate

Protein precipitation UNIFILTER FF



> Protein precipitation UNIFILTER FF

Protein precipitation UNIFILTER® FF

The Whatman Protein Precipitation UNIFILTER FF (Fast Flow)is optimized for removing acetonitrile-precipitated proteins from plasma or serum samples. Made with 2 mL, 96 well, rigid glass-filled polypropylene microplates, the Whatman Protein Precipitation UNIFILTER is both robust and chemically resistant.

The plates contain specially formulated dual membranes with two distinct layers. The top layer acts as a prefilter to remove coarse particulates. The bottom layer is oleophobic for retaining the well contents without dripping. This provides a final filter for removing fine particulate matter when a vacuum is applied. The plates are available in the following well bottom formats: Standard; Fast Flow; Round Bottom and "V" Bottom.

Features and Benefits

	96 samples purified at the same time
	Purified samples available in less than 10 minutes
	Precipitation and filtration in the same well
	No laborious pipetting and/or centrifugation required and minimal liquid handling
	Dual Whatman filter media
	Ensures no fluid loss during incubation and fast flow during filtration
	Over 98% protein removal
	Sample volumes up to 150µL for plasma and 200µL for serum
	SBS compatible with the exception of the "V" bottom plate
	Robotics friendly

Ordering Information - Protein Precipitation UNIFILTER® FF
Catalogue Number	Well Format	Well Volume	Plate Material	Well Bottom	Quantity/Case
7720-7235	96	2 mL	Glass Polypropylene	Standard	1
7720-7236	96	2 mL	Glass Polypropylene	Fast Flow	5
7701-5750	96	750 µL	Natural Polypropylene	Round Bottom	25
7701-5200	96	2 mL	Natural Polypropylene	Round Bottom	25



> 384 Well DNA binding UNIFILTER

384 Well DNA binding UNIFILTER®
The 384 Well DNA Binding UNIFILTER plate effectively binds and purifies DNA molecules. It provides highly reproducible results with yields exceeding 2 µg/well, from bind-wash-elute processing with collection by filtration. Minimal liquid hang up allows for reduced elution volume, enabling DNA concentration as high as 150 ng/µL. Further ethanol precipitation is unnecessary. The DNA is ready to use.

Features and Benefits

	High efficiency bind-wash-elute processing with collection by filtration
	simplifies automation with no cross contamination
	Highly reproducible results yielding DNA purity exceeding 2µg per well sufficient for sensitive downstream applications
	Minimal liquid hang up allows for reduced elution volume with DNA concentration as high as 150ng/µL

Ordering Information - 384 Well DNA Binding UNIFILTER®
Catalogue Number	Well Format	Well Volume (µL)	Plate Material	Filter Media	Quantity/Case
7700-2110	384	100	Clear Polystyrene	DNA Binding	50
7701-1100	384	100	Clear Polystyrene	N/A	50

Flexible capmats



> Flexible capmats

Flexible capmats
Whatman flexible capmats individually seal the top of each well. Capmats may be used on either filter or collection microplates.

Ordering Information
Capmats
Catalogue Number	Well Format	Capmat Material	Microplate Compatibility	Quantity/Case
7704-0004	96	Square Format EVA	2 mL microplates	100
7704-0005	96	Round Format EVA	750 µL and 800 µL microplates	100
7704-0006	48	Rectangular Format EVA	5 mL microplates	100
7704-0007	24	Square Format Santoprene	10 mL microplates	100
7704-0015	384	Square Format Santoprene	400 µL microplate	100
Pierceable Capmats
7704-0104	96	Square Format Silicone	2 mL microplates	50
7704-0105	96	Round Format Silicone	300 µL, 750 µL and 800 µL microplates	50
7704-0115	384	Square Format Silicone	100 µL and 400 µL microplates	50
Venting Capmats (autoclavable)
7704-0014	24	BugStopper Venting	10 mL microplates	5

Chromatography

Chromatography cellulose media



> Chromatography cellulose media

Whatman offers an extensive product line of chromatography cellulose media for applications ranging from the separation of biopolymers such as proteins, peptide and hormones to the purification of monoclonal antibodies, vaccines, synthetic pharmaceuticals and agrochemicals.

Technological innovations in product development and manufacturing processes and a dedication to excellence are combined to ensure batch-to batch reproducibility of these chromatography cellulose media.

Bulk silica media for column chromatography



> Bulk silica media for column chromatography

The Whatman bulk silica media is used for low pressure columns. Same base silica and chemistries as those used for Whatman TLC plates. Particularly useful for scaling up separations optimized by TLC. In addition, Whatman offers an all-purpose media for general column chromatography. Applicable for preparative low pressure column separations scaled up from Partisil analytical HPLC columns.

Features and Benefits

	High resolution
	Good flow characteristics
	High surface area

Ordering Information
Catalogue Number	Product	Particle Size (µm)	Other Specifications	Weight
Bulk Silica Media for Low Pressure Column Chromatography
4771-010	LPS-2 low pressure silica gel	37–53	Adsorption	450 m2/g; may be dry packed	1 kg
4776-001	LRP-2 ODS bonded silica gel	37–53	Reversed phase	LPS-2 silica bonded with 16% C; - may be dry packed; end-capped	100 g
4776-005	LRP-2 ODS bonded silica gel	37–53	Reversed phase	LPS-2 silica bonded with 16% C; - may be dry packed; end-capped	500 g

Ordering Information
Catalogue Number	Product	Particle Size (µm)	Other Specifications	Weight
Bulk Silica Media for General Column Chromatography
4132-100	ODS-3 (C-18)	32-75	Reversed Phase	Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load	100 g
4132-301	ODS-3 (C-18)	32-75	Reversed Phase	Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load	1 kg
4790-010	Silica, 60Å	230-400 mesh - (38-63)	Adsorption	Bulk media for column or - dry chromatography	1 kg
4790-050	Silica, 60Å	230-400 mesh - (38-63)	Adsorption	Bulk media for column or - dry chromatography	5 kg
4790-250	Silica, 60Å	230-400 mesh - (38-63)	Adsorption	Bulk media for column or - dry chromatography	25 kg
4791-005	Silica, 60Å	70-230 mesh - (63-212)	Adsorption	Bulk media for column or - dry chromatography	500 g