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Multiwell accs.
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UNIFILTER® Filtration
Microplates
The patented Whatman UNIFILTER microplates with filter-bottom wells are
convenient and ready to use. Available in 24, 96 and 384 well formats,
UNIFILTER microplates offer a choice of filter media to meet exact
application requirements.
The unique drip director design of Whatman UNIFILTER microplates
ensures precise collection of the filtrate to allow for further
processing and analysis. UNIFILTER microplates are available in a range
of well volumes from 100 µL to 10 mL.
Features and Benefits
- No crosstalk. Patented integral filter design prevents
well-to-well cross contamination
- Economical to use. Wide range of well volume options ensures
efficient use of materials
- Better control. Choice of filter media allows control of the
flow rates and retention characteristics
- Versatile. A broad range of filtration media is available
including glass fibre, polypropylene, cellulose nitrate, cellulose
acetate, nylon and ion exchange cellulose
| Typical Data - UNIFILTER |
| Filter Media |
Flow Rate* |
Protein Binding |
Hydrophilic |
Solvent Resistance |
Physical Strength |
Thermal Resistance °C |
General Comments |
| Cellulose Nitrate (CN) |
4 |
High |
Yes |
Poor |
Brittle |
<125 |
Highly adsorptive membrane (CN)
typically used for DNA/RNA/ protein hybridization, also for
ELISA and RIA-based assays. |
| Cellulose Acetate (CA) |
3 |
Low |
Yes |
Poor |
Moderate |
<120 |
Typically used for low protein binding
applications, good wet strength. General purpose microbiological
filter. |
| Polypropylene (PP) |
2 |
Negligible |
No |
Very Good |
Good |
<80 |
Typically used for prefiltration.
Sensitive to gamma sterilization. Very low extractables,
chemically inert. |
| Polyvinylidene fluoride (PVDF)
Hydrophilic** |
4 |
Low |
Yes |
Good |
Good |
<135 |
Low protein binding, good chemical
resistance. |
| Glass Microfiber (GF) |
5 |
Moderate |
Yes |
Very Good |
Poor |
High |
Wide range available. Typically used as
absorptive or adsorptive wicking media and prefilters. Excellent
particle retention and resistance to clogging. Used for DNA
binding. |
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96 Well 800 µL UNIFILTER
microplate
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| > 96 Well 800 µL
UNIFILTER microplate |
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96 Well 800 µL UNIFILTER microplate
The 800µL UNIFILTER is the microplate most typically used in
purifications, isolations and separation of biomolecules, particularly
DNA.
The microplate has a well volume of 800µL which is ideal for standard
DNA plasmid miniprep chemistries. The choice of short or long drip
directors is application specific. The UNIFILTER 800µL is constructed
from rigid high grade polystyrene.
| 96 Well 800 µL UNIFILTER®
Microplate |
| Catalogue Number |
Well Format |
Well Volume (µL) |
Plate Material |
Drip Director |
Filter Media |
Quantity/Case |
| 7700-1801 |
96 |
800 |
Clear Polystyrene |
Short |
Whatman GF/C |
25 |
| 7700-1804 |
96 |
800 |
Clear Polystyrene |
Short |
25–30 µm melt blown polypropylene |
25 |
| 7700-1806 |
96 |
800 |
Clear Polystyrene |
Short |
0.45 µm hydrophilic PVDF |
25 |
| 7700-1808 |
96 |
800 |
Clear Polystyrene |
Short |
0.45 µm Cellulose Acetate |
25 |
| 7700-1818 |
96 |
800 |
Clear Polystyrene |
Short |
5–7 µm melt blown polypropylene |
25 |
| 7700-2801 |
96 |
800 |
Clear Polystyrene |
Long |
Whatman GF/C |
25 |
| 7700-2803 |
96 |
800 |
Clear Polystyrene |
Long |
Whatman GF/B |
25 |
| 7700-2804 |
96 |
800 |
Clear Polystyrene |
Long |
25–30 µm melt blown polypropylene |
25 |
| 7700-2805 |
96 |
800 |
Clear Polystyrene |
Long |
0.45 µm PP membrane |
25 |
| 7700-2806 |
96 |
800 |
Clear Polystyrene |
Long |
0.45 µm hydrophilic PVDF |
25 |
| 7700-2808 |
96 |
800 |
Clear Polystyrene |
Long |
0.45 µm Cellulose Acetate |
25 |
| 7700-2809 |
96 |
800 |
Clear Polystyrene |
Long |
0.45 µm Nylon Positive |
25 |
| 7700-2810 |
96 |
800 |
Clear Polystyrene |
Long |
DNA binding plate |
25 |
| 7700-2811 |
96 |
800 |
Clear Polystyrene |
Long |
Whatman GF/D |
25 |
| 7700-2817 |
96 |
800 |
Clear Polystyrene |
Long |
10–12 µm melt blown polypropylene |
25 |
| 7720-2830 |
96 |
800 |
Clear Polystyrene |
Long |
Lysate clarification plate |
25 |
| 7700-2828 |
96 |
800 |
Clear Polystyrene |
Long |
Whatman oleophobic PKP |
10 |
| 7770-0062 |
96 |
800 |
Clear Polystyrene |
Long |
25 µm melt blown polypropylene over
0.45 µm PP membrane |
25 |
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membrane filters
Whatman brings to the laboratory user a range of membrane filters whose
advanced technical specifications makes them today's preferred choice
for a wide range of applications. The membrane filters offer accurately
controlled pore size distribution and higher strength and flexibility
which ensure reproducibility and consistency. The Whatman membrane
filter range includes pore sizes (from 0.02 to 5µm) with a wide
selection of membrane filters. Sterile and autoclave packs are available
for specialized applications. Colored and gridded types are also
available.To access the range of Whatman Membrane Filters, please
click on the links on the navigation box to the right.
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Anodisc
inorganic membranes
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| > Anodisc
inorganic membranes |
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The Anopore® inorganic membrane is ideal for a wide range of
laboratory filtration applications. This unique material has a
precise, non-deformable honeycomb pore structure with no lateral
crossovers between individual pores.that filters at precisely
the stated cutoff, allowing no larger sized particles to pass
through the membrane. The Anopore inorganic membrane is composed
of a high purity alumina matrix that is manufactured
electrochemically. The membrane also exhibits low protein
binding, has minimal autofluorescence, is non-toxic and supports
cellular growth.
The precise pore structure and narrow pore size distribution
of the Anopore membrane ensure a high level of particle removal
efficiency. Micro-organisms and particulate material are
captured on the surface of the membrane for subsequent analysis
by light or electron microscopy. When wet, the membrane is
virtually transparent, which means that retained particles do
not need to be transferred to another surface before microscopic
examination.
>
Anodisc pore structure
The membrane is hydrophilic and is compatible with most
solvents and aqueous material. No monomers, plasticizers,
adhesives, surfactants or wetting agents are used in the
manufacturing process, which eliminates sample contamination and
ensures low protein binding and minimal loss of sample.
The Anopore membrane is supplied in the form of Anodisc
membrane filters. The membrane is peripherally bonded to an
annular polypropylene ring (except the 13 mm diameter disc) for
ease of handling and is suitable for both vacuum and pressure
filtration.
Anopore is available in 3 nominal pore sizes: 0.02µm, 0.1µm
and 0.2µm and in 3 disposable syringe filter housings: 13mm,
25mm and 47mm.
Features and Benefits
- High pore density and narrow pore size distribution make
it an extremely precise membrane
- Wide solvent compatibility reduces the need to stock a
variety of membranes in the laboratory
- No additives used in the manufacturing process ensures
minimal extractables and no sample contamination
- Extremely low protein binding minimizes sample loss
- Virtually transparent when wet making it ideal for
microscopy studies
Applications
- HPLC mobile phase filtration and degassing
- Ultra cleaning of solvents
- Gravimetric analysis
- Liposome extrusion
- Scanning electron microscopy studies
- Bacterial analysis by epifluorescence light microscopy
- Micrometer and nanometer filtration
- Metal nanorods formation
| Typical Data |
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Anodisc 13 |
Anodisc 25 |
Anodisc 47 |
| Average Membrane Thickness |
60 µm |
60 µm |
60 µm |
| Membrane Diameter |
13 mm |
21 mm |
43 mm |
| Membrane Type |
Anopore |
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aluminum oxide |
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| Support Ring Material |
None |
Polypropylene |
Polypropylene |
| Construction Process |
None |
Thermal weld |
Thermal weld |
| Protein Adsorption |
Low |
Low |
Low |
| Burst Strength |
65–110 psi |
65–110 psi |
65–110 psi |
| Maximum Service Temperature |
400°C |
400°C |
400°C |
| Porosity |
25-50% |
25-50% |
25-50% |
| Autoclavable |
Yes |
No |
No |
| Refractive Index |
1.6 |
1.6 |
1.6 |
| Ordering Information
- Diameter (mm) |
| Diameter (mm) |
Pore Size (µm) |
Membrane |
Catalogue Number |
Hydrophilic |
Protein Binding |
Solvent Resistance |
Quantity/Pack |
| 13 |
Anodisc 13* |
0.02 |
6809-7003 |
Yes |
Low |
Very Good |
100 |
| 13 |
Anodisc 13* |
0.1 |
6809-7013 |
Yes |
Low |
Very Good |
100 |
| 13 |
Anodisc 13* |
0.2 |
6809-7023 |
Yes |
Low |
Very Good |
100 |
| 25 |
Anodisc 25 |
0.02 |
6809-6002 |
Yes |
Low |
Very Good |
50 |
| 25 |
Anodisc 25 |
0.1 |
6809-6012 |
Yes |
Low |
Very Good |
50 |
| 25 |
Anodisc 25 |
0.2 |
6809-6022 |
Yes |
Low |
Very Good |
50 |
| 47 |
Anodisc 47 |
0.02 |
6809-5002 |
Yes |
Low |
Very Good |
50 |
| 47 |
Anodisc 47 |
0.1 |
6809-5012 |
Yes |
Low |
Very Good |
50 |
| 47 |
Anodisc 47 |
0.2 |
6809-5022 |
Yes |
Low |
Very Good |
50 |
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ELISA UNIFILTER®
The Whatman ELISA plate allows researchers to use the binding
characteristics of nitrocellulose in the standard SBS 8 x 12 multiwell
format. It is also suitable for other types of ligand binding
applications such as hybridoma supernatant screening. Solutions are
easily vacuumed to waste using a vacuum manifold.
Chemiluminescent assays can be read in the plate. If the ELISA product
is eluted then centrifugation is preferred. This microplate is
compatible with automated systems.
| Ordering Information - ELISA
UNIFILTER® |
| Catalogue Number |
Well Format |
Well Volume |
Plate Material |
Filter Media |
Well Bottom |
Quantity/Case |
| 7700-3307 |
96 |
350 µL |
White Polystyrene |
0.45µm CN * |
Filter |
50 |
| 7701-1350 |
96 |
300 µL |
Clear Polystyrene |
N/A |
Flat |
50 |
| 7701-5200 |
96 |
2 mL |
Natural Polypropylene |
N/A |
Round |
25 |
| 7705-0107 |
UniVac™ Vacuum to Collect
Manifold, acrylic |
1 |
| 7704-0001 |
Clear Polyester ThinCold
Sealing Film Adhesive Backing |
100 |
| * CN = Cellulose nitrate |
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| > Protein precipitation
UNIFILTER FF |
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Protein precipitation UNIFILTER® FF
The Whatman Protein Precipitation UNIFILTER FF (Fast Flow)is
optimized for removing acetonitrile-precipitated proteins from plasma or
serum samples. Made with 2 mL, 96 well, rigid glass-filled polypropylene
microplates, the Whatman Protein Precipitation UNIFILTER is both robust
and chemically resistant.
The plates contain specially formulated dual membranes with two
distinct layers. The top layer acts as a prefilter to remove coarse
particulates. The bottom layer is oleophobic for retaining the well
contents without dripping. This provides a final filter for removing
fine particulate matter when a vacuum is applied. The plates are
available in the following well bottom formats: Standard; Fast Flow;
Round Bottom and "V" Bottom.
Features and Benefits
- 96 samples purified at the same time
- Purified samples available in less than 10 minutes
- Precipitation and filtration in the same well
- No laborious pipetting and/or centrifugation required and
minimal liquid handling
- Dual Whatman filter media
- Ensures no fluid loss during incubation and fast flow during
filtration
- Over 98% protein removal
- Sample volumes up to 150µL for plasma and 200µL for serum
- SBS compatible with the exception of the "V" bottom plate
- Robotics friendly
| Ordering Information -
Protein Precipitation UNIFILTER® FF |
| Catalogue Number |
Well Format |
Well Volume |
Plate Material |
Well Bottom |
Quantity/Case |
| 7720-7235 |
96 |
2 mL |
Glass Polypropylene |
Standard |
1 |
| 7720-7236 |
96 |
2 mL |
Glass Polypropylene |
Fast Flow |
5 |
| 7701-5750 |
96 |
750 µL |
Natural Polypropylene |
Round Bottom |
25 |
| 7701-5200 |
96 |
2 mL |
Natural Polypropylene |
Round Bottom |
25 |
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Bulk silica media for
column chromatography
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| > Bulk silica media for
column chromatography |
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The Whatman bulk silica media is used for low pressure columns. Same
base silica and chemistries as those used for Whatman TLC plates.
Particularly useful for scaling up separations optimized by TLC. In
addition, Whatman offers an all-purpose media for general column
chromatography. Applicable for preparative low pressure column
separations scaled up from Partisil analytical HPLC columns.
Features and Benefits
- High resolution
- Good flow characteristics
- High surface area
| Ordering Information |
| Catalogue Number |
Product |
Particle Size (µm) |
Other Specifications |
Weight |
| Bulk Silica Media for Low
Pressure Column Chromatography |
| 4771-010 |
LPS-2 low pressure silica gel |
37–53 |
Adsorption |
450 m2/g; may be dry packed |
1 kg |
| 4776-001 |
LRP-2 ODS bonded silica gel |
37–53 |
Reversed phase |
LPS-2 silica bonded with 16% C; - may be
dry packed; end-capped |
100 g |
| 4776-005 |
LRP-2 ODS bonded silica gel |
37–53 |
Reversed phase |
LPS-2 silica bonded with 16% C; - may
be dry packed; end-capped |
500 g |
| Ordering Information |
| Catalogue Number |
Product |
Particle Size (µm) |
Other Specifications |
Weight |
| Bulk Silica Media for
General Column Chromatography |
| 4132-100 |
ODS-3 (C-18) |
32-75 |
Reversed Phase |
Octadecyl-bonded; end-capped; -
polymeric; 10.5% carbon load |
100 g |
| 4132-301 |
ODS-3 (C-18) |
32-75 |
Reversed Phase |
Octadecyl-bonded; end-capped; -
polymeric; 10.5% carbon load |
1 kg |
| 4790-010 |
Silica, 60Å |
230-400 mesh - (38-63) |
Adsorption |
Bulk media for column or - dry
chromatography |
1 kg |
| 4790-050 |
Silica, 60Å |
230-400 mesh - (38-63) |
Adsorption |
Bulk media for column or - dry
chromatography |
5 kg |
| 4790-250 |
Silica, 60Å |
230-400 mesh - (38-63) |
Adsorption |
Bulk media for column or - dry
chromatography |
25 kg |
| 4791-005 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
500 g |
| 4791-010 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
1 kg |
| 4791-050 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
5 kg |
| 4791-250 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
25 kg |
NEW Purasil 60Å Silica Gel
Silica Gel Media for Flash Chromatography
Whatman Purasil high purity silica gel provides an excellent separation
medium for flash chromatography purification of target molecules. Narrow
particle size distribution and minimal fines enable fast separations
with no loss of chromatographic performance.
Features and Benefits
- High resolution
- Excellent flow rates
- Direct scalability
| Typical Data |
| Iron Content |
<0.02% |
| Chloride Content |
<0.10% |
| Loss on Drying |
<7% |
| pH (10% suspension) |
7 ± 0.5 |
| Pore Volume |
60–80 mL/g |
| Surface Area |
500–600 m2/g |
| Ordering Information |
| Catalogue Number |
Product |
Dimensions |
| 4745-010 |
230–400 mesh (38–63 µm) |
1 kg |
| 4745-250 |
230–400 mesh (38–63 µm) |
25 kg |
| 4746-010 |
70–230 mesh (63–210 µm) |
1 kg |
| 4746-250 |
70–230 mesh (63–210 µm) |
25 kg |
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Collection plates
Whatman microplates for collection and analysis are available in 24, 48,
96 and 384 well formats, all unique to Whatman. These microplates are
manufactured from polystyrene, polypropylene and Multi-Chem™ materials
to accommodate a wide range of sampling and storage applications.
To access the range of Whatman Collection Plates, please click on the
links on the navigation box to the right.
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