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TEL: +32 16 58 90 45
Fax :+ 32 16 50 90 45
GENTAUR Europe
tel+32 2 732 5688
fax+32 2 732 4414
Av.
de l' Armée 68
B-1040 BRUSSELS
BELGIUM

GENTAUR France
tel 01 43 25
01 50
fax01 43 25
01 60
9, rue
Lagrange
75005 PARIS
FRANCE

GENTAUR Italy
tel 02 36 00
65 93
fax 02 36 00
65 94
20135 MILANO
ITALY

GENTAUR Germany
tel +49 241
6085 13140
fax +49 241
6085 33033
Forckenbeckstraße 6,
D-52074
Aachen
GERMANY
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MALDI-TOF Determination of the absolute molecular weight of the protein
subunit(s)
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You provide us with:
At least 10 pmol purified native protein (for subunit molecular weigths up
to 30 kDA) or 20 pmol (for subunit molecular weigths up to 100 kDA). For
larger proteins more protein is needed. The protein may be ammonium sulfate
precipitated or lyophilized. Please enclose a photo of an analytical
SDS-PAGE, mention the %age of the gel, clearly mark the band which
corresponds to the purified protein, and specify the molecular weight marker
and the estimated molecular weight of the protein of interest. Recommend a
suitable buffer for dialyzing (ammonium sulfate precipitate) or dissolving
(lyophilized material) the protein. If this is not a standard buffer, add
your buffer in sufficient amount (esp. for dialysis). Mention special
requirements of your protein with respect to solubility and stability
(especially important for membrane proteins).
Please ship the protein without cooling (lyophilized protein) or on blue ice
(ammonium sulfate precipitate) to:
Gentaur
Avenue de l'Armée 68/B4
B-1040 Brussels
Belgium
Our service:
Sample preparation and MALDI-TOF Mass Spectrometry of the protein.
Determination of the absolute mass of the protein subunit(s).
Order Information:
Cat. Nr. DMW01
Absolute Molecular Weight Determination of purified protein by MALDI-TOF MS
Price: 225 Euro
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