MCHR & MCH
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Melanin Concentrating Hormone Receptor 1 and 2 (MCHR1 and MCHR2) Antibodies

 

Melanin-concentrating hormone (MCH) is a cyclic neuropeptide (human/rat 19 aa) that regulates a variety of functions in mammalian brain, in particular feeding behavior. MCH is synthesized in mainly in the lateral hypothalamus and zona incerta. MCH stimulates feeding, its level is unregulated in the hypothalamus of genetically obese mice, and mice lacking MCH eat less and are lean. MCH is thought to influence feeding and energy balance by acting downstream of leptin and the melanocortin system. However, molecular mechanisms of these diverse actions of MCH remain poorly understood, as its receptor was not identified.

Recently, an orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH. MCH receptor (MCHR1; human 402 aa, rat 353 aa) is predicted to contain 7 transmembrane domains, a feature typical of G-protein coupled receptors. It is primarily expressed in the ventromedial and dorsomedial nuclei of the hypothalamus. Moderate levels of MCHR are also found in the eye and skeletal muscle, tongue, and pituitary. MCHR binds MCH with sub-nanomolar affinity, and is stimulated by MCH to mobilize intracellular Ca and reduce forskolin-elevated cAMP levels.

Recently, a novel second human MCH receptor (MCH2R) has been cloned and characterized. MCH2R gene encodes a 340 aa protein with 38% identity with MCH1R. It has the basic characteristics of GPCR and belongs to class 1 (rhodopsin-like) GPCR superfamily. MCH2R transfected cells responds to MCH with an increase in intracellular Ca+2 levels, and increased cellular extrusion of protons. MCH2R is mainly expressed in the brain.

ADI has produced rabbit antibodies to MCH and MCHRs using specific peptide sequences. Purified MCH is also available.

 Items Antigen peptide location   Antibody Host  Ab Crossreactivity  Neat Antisera Cat #
(100 ul)		 Aff. Pure Ab
Cat #
(100 ug)		  * Control Peptide Cat#
(100 ug)
MCHR1 R/H, 16 aa, ~CT Rb R, H MCHR11-S MCHR11-A  MCHR11-P
 MCHR2 H, 19 aa, ~CT Rb H, Mo (M, R?) MCHR21-S  MCHR21-A  MCHR21-P
MCH H, MCH, 19 aa full length Rb H, M, R, F MCH51-S MCH51-A MCH51-P
 MCH H, full length, (cyclic Cys 7-16), Cat # MCH61-P (100 ug)
 Control Rabbit IgG  For use in ELISA, Western, Immunohisto. 20009-1 (1 mg)  


 

CP= Cytoplasmic domain; EC= extracellular domain; M=Mouse; R= Rat; H=Human, F=Fish, Mo=Monkey


"Neat Antisera" are the unpurified antiserum and it is suitable for ELISA and Western.
"Affinity pure" antibodies have been over the antigen-affinity column and recommended for immunohistochemical applications.
"Control peptides" can not be used for Western as they are very short peptides. They are intended for ELISA or antibody competition studies.