M-CSF mouse
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Specification sheet

Macrophage Colony Stimulating Factor, Recombinant, Mouse (M-CSF)

Catalog No:PC211-1 (2 micrograms) 180€
Catalog No:PC211-2 (10 micrograms) 275€
Catalog No:PC211-3 (1 mg) CALL/EMAIL
Source E.Coli
Formulation

The sterile filtered solution was lyophilized from 5 mM Tris, pH 8.0 + 100 mM NaCl.

Reconstitution Centrifuge briefly to bring the protein down to the bottom of the vial. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other buffers and stored at 4 degree Celsius for 1 week or stored at -20 degree Celsius for future use. Avoid repeated freeze-thaw cycles.
Storage The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degree Celsius. Reconstituted M-CSF should be stored in working aliquots at -20 degree Celsius.
Purity Greater than 98% by SDS-PAGE.
Biological Activity The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is less than 1.0 ng/mL, corresponding to a specific activity greater than 1 x 10^6 units/mg.
Description Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types including fibroblasts, macrophages and endothelial cells. This colony stimulation factor is involved in both the development and function of monocytes/macrophages. Recombinant Mouse M-CSF is a disulfide-linked homodimer composed of 18.2 kDa subunits of 156 amino acid residues.
Endotoxcin Endotoxin level is less than 0.1 ng/μg.
Amino Acid Sequence MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQNKACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Country of Origin USA