|
Specification sheet |
Macrophage Colony Stimulating Factor, Recombinant, Human
(M-CSF) Highly Purified |
|
Catalog No:PC111-1 |
(2 micrograms) |
180€ |
|
Catalog No:PC111-2 |
(10 micrograms) |
275€ |
|
Catalog No:PC111-3 |
(1 mg) |
CALL/EMAIL |
|
Source |
E.Coli |
| Formulation |
The sterile filtered solution was lyophilized from 3mM
Tris, pH 8.5. |
| Reconstitution |
We recommend a quick
spin followed by reconstitution in water to a concentration of 0.1-0.5mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 4
degree Celsius for 1 week or -20 degree Celsius for future use. |
| Storage |
The lyophilized
protein is stable for a few weeks at room temperature, but best stored at
-20 degree Celsius. Reconstituted M-CSF should be stored in working aliquots
at -20 degree Celsius. |
| Purity |
Greater than 98%
SDS-PAGE and HPLC analyses. |
| Biological
Activity |
The ED50
was determined by the dose-dependent stimulation of the proliferation of
mouse M-NSF-60 cells is >= 10 ng/mL, corresponding to a specific activity of
<=1 x 10^5 units/mg. |
| Description |
Macrophage Colony
Stimulating Factor (M-CSF) is produced by various cell types, including
fibroblasts, macrophages and endothelial cells. This colony stimulating
factor is involved in both the development and function of monocytes/macropahges.
Human M-CSF is a disulfide-linked homodimer composed of 18.4kD subunits of
158 amino acid residues. |
| Endotoxcin |
Less than 0.1 ng/ug
(1Eu/ug) |
| Country of
Origin |
USA |
.htm_cmp_ice010_hbtn.gif){kind=small}
.htm_cmp_ice010_hbtn.gif){kind=small}
