|
LuminMax-C™
Easy to Use Attomole
Luminometer |
|
The study and
use of bioluminescence and chemiluminescence
has increased dramatically in the recent
years. The applications have extended from
ATP-luciferase assay for cell viability
tests, to current DNA, genomic, and
proteomic analysis. Unlike a fluorescence
system, there is no need for an exogenous
light source. Luminescence is generated from
chemical reactions. Utilizing photoncounting
detector, counting the photons generated
from the reaction, luminescence assay has
become one of the most sensitive optical
detection method. LuminMax-C offers
excellent sensitivity, accuracy, ease of
use, compactness, and affordability.
LuminMax-C is a
compact model designed for highly sensitive
chemiluminescence and bioluminescence
detection. The system accurately quantifies
the luminescence intensity in a 96-well
microplate (black or white). The microplate
has aclear bottom; therefore, the
luminescence can be detected from the bottom
of the well. Because it uses
state-of-the-art photoncounting multiplier
tube as detector, the system is extremely
sensitive. LuminMax-C has the ability to
count the number of photon generated from
the reaction, it means that it can detect
very small amount of analyte in the samples.
A CD, with user-friendly software, is
provided for easy installation. LuminMax-C
utilizes PC or notebook as its
microprocessor. The system is interfaced to
a computer by a simple plug-in (USB or
serial port) connection. After click on the
"Go!" botton, the system automatically and
quickly scans all of the selected microwells
and displays the results. The resulting data
is displayed as a spreadsheet in
Microsoft-Excel format. The data, reported
as number of photon counts or relative light
unit (RLU), is displayed as it is collected.
|
Optical detection: |
Chemi- or bio-luminescence |
|
Plate format: |
Microplate (96 wells) |
|
Sensitivity: |
1 attomole HRP and ATP |
|
Optical wavelength: |
300 ~ 680 nm |
|
Dynamic range: |
Seven decades |
|
Optical detector: |
Photoncounting PMT |
|
Cross-talk: |
< 6 x 10-5 |
|
Operation: |
Automoted scanning any or all wells;
Integration time (0.01 ~ 10.0 S) per
well; Adjustable number of scans;
Adjustable delay for kinetic study |
|
Interface: |
Serial or USB
to PC or Notebook (PC or Notebook
not included) |
|
Software: |
CD with user friendly software;
Display all microwell data |
|
Data output: |
Excel format in MS Windows |
|
Power requirement: |
115V, 60Hz |
|
Dimension: |
12"
W x 11" L x 5.8" H
(30cm W x 28cm L x 15cm H) |
|
Weight: |
19.8 lbs. (9 kgs.) |
Top
|
♦ |
ATP assay |
|
♦ |
Luciferase assay |
|
♦ |
Immunoassay & proteomics |
|
♦ |
Nucleic acid, DNA assay & Genomics |
|
♦ |
Clinical diagnostics |
|
♦ |
Genomic analysis |
|
♦ |
VitotTox Toxicity test |
|
♦ |
Cell viability test |
|
♦ |
Restaurant sanitary test |
|
♦ |
Biotechnology research
laboratories |
|
♦ |
University biological and
biochemical laboratories |
|
♦ |
Government biological and
biomedical laboratories |
|
♦ |
Hospital
research laboratories |
|
♦ |
Food industry, environmental or
forensic testing |
|
|
|
Cell Quantification and ATP
Tests
LuminMax-Q-Cell:
Luminescent Cell Viability Assay
155 Euro
1 vial
LuminMax-ES, Luciferase and Substrate (luciferin) (lyophilized)
10 ml
LuminMax-CB, Cell Buffer
100µl per
assay (microwell),
the material
is sufficient for 100 assays in 96-well plate
ATP:
for Sensitivity and Standard Tests
175 Euro
1 vial (400
µl, 100mM)
20µl for a
series of dilution for a standard (calibration) test,
the material
is sufficient for standard tests for 20 times
·
The lyophilized LuminMax-ES and Cell Buffer
should be stored at -20oC for
long-term (over one week) storage. For frequent use, it can be stored at 4oC;
however, it may lose some activity.
ATP
Assay Protocol:
Purpose: To
generate ATP standard curve
Material:
1.
ATP 100mM
2.
LuminMax-CB buffer
3.
LumiMax-ES substrate (lyophilized)
4.
Bottom clear 96 well microplate or strip: Must be compatible with the
luminometer
5.
Nuclease-free water
Reagent Preparation:
1.
Thaw the LuminMax-CB Buffer and equilibrate to room temperature prior to
use.
2.
Equilibrate the lyophilized LuminMax-ES Substrate to room temperature
prior to
use.
3.
Transfer the appropriate volume of Buffer into the bottle containing
Substrate to
reconstitute the lyophilized enzyme/substrate mixture. This is the Quantitative
Cell (or Signal) Reagent.
Experiment:
1.
Prepare 1uM ATP in culture medium, buffer or nuclease-free water.
(100µl of 1µM ATP solution contains 10-10 moles ATP).
2.
Prepare tenfold serial dilutions of ATP in culture medium, buffer or
nuclease-free
water (e.g. 1µM to 10nM). (Dilute further for sensitivity test of the
luminometer.)
3.
Dispense 100µl of ATP solutions in microwell plate.
4.
Add a volume of Q-Cell reagent equal to the volume of ATP.
5.
Mix contents for 2 minutes on a shaker.
6.
Incubate the plate at room temperature for 5 or 10 minutes.
7.
Read luminescence with integration time of 100 ms or 1.0 second.
Example of ATP Standard Curve Assay
|
Purpose: To Evaluate
sensitivity with ATP assay using LuminMax-C |
|
|
|
|
Material: |
|
|
|
|
|
|
|
|
|
1. ATP:
100mM, 400ul, store at -20C, thawed, Store 4C. |
|
|
|
|
|
Prepare (1)20ul + 1.98ml nuclease-free water = 1mM; |
|
|
|
|
(2)
20ul of (1) +1.98 ml of water = 10uM |
|
|
|
|
|
|
(3)
0.1ml of (2)+ 0.9ml water =1uM, 100ul 1uM ATP=10 -10moles ATP
; |
|
|
|
(4)
20ul of (3)+1.98ml water =10nM; |
|
|
|
|
|
|
(5)
20ul of (4)+1.98ml water = 100pM; |
|
|
|
|
|
(6)
200ul of (5)+200ul water = 50pM; |
|
|
|
|
|
|
(7)
200ul of (6) +200ul water = 25pM |
|
|
|
|
|
2. |
Cell
buffer, 10ml |
|
|
|
|
|
|
|
Thaw
and equilibrate the lyophilized substrate and buffer to RT. |
|
|
|
|
Transfer all buffer into the substrate bottle. Mix. |
|
|
|
|
|
Dispense 1.5ml per vial if needed. Store at 4C. |
|
|
|
|
Experiment : |
|
|
|
|
|
|
|
|
|
1. Dispense 100ul of
ATP in the microwell. |
|
|
|
|
|
2. Add 100ul of
LuminMax-Q-Cell equal to the volume of ATP standard present in each
well. |
|
3. Mix for 2 minutes
on orbital shaker. |
|
|
|
|
|
|
4. Allow the plate
to incubate at RT for 5 or 10 min. |
|
|
|
|
5. Record
luminescent on LuminMax for 100 ms or 1 second. |
|
|
|
|
Result: |
|
|
|
|
|
|
|
|
|
Microwell |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
|
Concentration, 100ul |
0 |
25pM |
50pM |
100pM |
10nM |
100nM |
1uM |
10uM |
|
5 min
(100ms) |
3 |
9 |
20 |
57 |
141 |
590 |
5662 |
40497 |
|
10 min
(1 second)' |
40 |
137 |
243 |
433 |
1861 |
5925 |
57954 |
409921 |
Cell Assay Protocol:
Cell
viability assay is similar to ATP assay protocol, instead of using ATP as
analyte, cell sample is input to the microwell. Depend on the cell types; each
cell may contain 10-15
- 10-18
mole of ATP. Once the cell titer (Cells per microwell) vs. Luminescence
Signal is calibrated, the method can be used to correlate cell number
with luminescent output.
* See Application Note for further information
| 
