Liver cancer
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   Category Human tissue array
   Cat. No. CS
   Product description Liver cancer
   Data sheet CS1.pdf
CS1.xls
   
   Images
  general_information
   No. of samples 59
   Text
Current version: CS1
Liver cancer: 59 cores

Price : 5 slide for 600 €

CS1  Human, liver cancer
               
  Age Sex Organ Diagnosis TNM Stage No. of
CSN1#
1 53 F skin metastatic HCC TxNxM1 ⅣB .
2 57 M liver HCC T3N0M0 ⅢA 104
3 41 M liver HCC T3N0M0 ⅢA .
4 45 M skin metastatic HCC TxNxM1 ⅣB .
5 54 F chest wall metastatic HCC TxNxM1 ⅣB .
6 40 M lumbar vertebra metastatic HCC TxNxM1 ⅣB .
7 52 M liver HCC T3N0M0 ⅢA 112
8 43 M bile duct HCC T4NxM0 ⅣA .
9 51 M colon metastatic HCC TxNxM1 ⅣB .
10 49 M bile duct HCC T4NxM0 ⅣA .
11 56 M liver HCC T3N0M0 ⅢA .
12 56 F lung metastatic HCC TxNxM1 ⅣB .
13 40 M lymph node metastatic HCC T3N1M0 ⅢB .
14 37 M liver HCC T4NxM1 ⅣB 131
15 75 M liver HCC T1N0M0 Ⅰ 132
16 37 M neck metastatic HCC TxNxM1 ⅣB .
17 32 M liver HCC T3N0M0 ⅢA 133
18 50 M femur metastatic HCC TxNxM1 ⅣB .
19 57 M liver HCC T3N0M0 ⅢA 134
20 62 M lung metastatic HCC TxNxM1 ⅣA .
21 61 M lung metastatic HCC TxNxM1 ⅣB .
22 65 M liver moderately differentiated HCC T2N0M0 Ⅱ 135
23 52 M liver moderately differentiated HCC T1N0M0 Ⅰ 136
24 65 M liver poorly differentiated HCC T2N0M0 Ⅱ 137
25 72 M liver well & poorly differentiated HCC T2N0M0 Ⅱ 138
26 58 F liver well differentiated HCC T2N0M0 Ⅱ 139
27 52 M liver well differentiated HCC T2N0M0 Ⅱ 140
28 40 M liver moderately differentiated HCC T2N0M0 Ⅱ 141
29 53 F liver moderately differentiated HCC T3N0M0 ⅢA 142
30 67 M liver moderately differentiated HCC T2N0M0 Ⅱ 143
31 58 F liver moderately differentiated HCC T2N0M0 Ⅱ .
32 20 M liver well differentiated HCC T4N0M0 ⅣA .
33 48 M liver moderately differentiated HCC T2N0M0 Ⅱ .
34 47 M liver HCC T1N0M0 Ⅰ 144
35 35 M omentum metastatic HCC TxNxM1 ⅣB .
36 69 F liver moderately differentiated HCC T2N0M0 Ⅱ .
37 65 M liver well differentiated HCC T2N0M0 Ⅱ 145
38 63 F liver moderately differentiated HCC T2N0M0 Ⅱ 146
39 60 F liver poorly differentiated HCC T2N0M0 Ⅱ 147
40 65 F liver moderately differentiated HCC T3N0M0 ⅢA 148
41 69 F liver poorly differentiated HCC T3N0M0 ⅢA .
42 66 F liver well differentiated HCC T2N0M0 Ⅱ 149
43 65 F liver moderately differentiated HCC T1N0M0 Ⅰ 150
44 64 M liver poorly differentiated HCC T3N0M0 ⅢA .
45 59 M liver moderately differentiated HCC T3N0M0 ⅢA .
46 64 M liver moderately differentiated HCC T2N0M0 Ⅱ 151
47 51 M liver moderately differentiated HCC T4N0M0 ⅢA 152
48 65 F liver moderately differentiated HCC T2N0M0 Ⅱ 153
49 60 M adrenal gland metastatic HCC TxNxM1 ⅣB .
50 44 M liver moderately differentiated HCC T1N0M0 Ⅰ .
51 41 M liver moderately differentiated HCC T2N0M0 Ⅱ .
52 51 M liver HCC T2N0M0 Ⅱ 154
53 49 M liver well differentiated HCC T4N0M0 ⅣA 155
54 57 F liver well differentiated HCC T2N0M0 Ⅱ 156
55 57 M liver moderately differentiated HCC T3N0M0 ⅢA 157
56 43 M liver  moderately differentiated HCC T2N0M0 Ⅱ .
57 50 M liver poorly differentiated HCC T2N0M0 Ⅱ 158
58 43 M liver moderately differentiated HCC T2N0M0 Ⅱ 159
59 60 F liver poorly differentiated HCC T4N0M0 ⅣA 160
60 carbon particle
               
HCC : hepatocellular carcinoma          
TNM and stage classification : liver cancer (AJCC 5th Edition)      

General Information of the Tissue Array Slide :

Applications

  • 􀁺 Immunohistochemistry
  • 􀁺 In situ hybridization, RNA or DNA
  • 􀁺 Fluorescent in situ hybridization (FISH)
  • 􀁺 In situ PCR, RNA or DNA
  • 􀁺 TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • 􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • 􀁺 Tissue array slides are shipped in room temperature.
  • 􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

  • 􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
  • 􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • 􀁺 Cleared by xylene, three changes for 1 hour each.
  • 􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
  • 􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • 􀁺 Dry slides for 1 hour in a oven at 60 degree.
  • 􀁺 Dewax slides in xylene for 4 minutes x 5 times
  • 􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • 􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

  • 􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

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