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Chromatography cellulose media



> Chromatography cellulose media

Whatman offers an extensive product line of chromatography cellulose media for applications ranging from the separation of biopolymers such as proteins, peptide and hormones to the purification of monoclonal antibodies, vaccines, synthetic pharmaceuticals and agrochemicals.

Technological innovations in product development and manufacturing processes and a dedication to excellence are combined to ensure batch-to batch reproducibility of these chromatography cellulose media.

Advanced Ion Exchange Cellulose
Whatman Advanced Ion Exchange Cellulose (AIEC) and Column Chromatography (CC) products are ideal for separation of biopolymers and suitable for a wide variety of applications. Available as:

	Preswollen microgranular AIEC for high load capacity fast kinetics and resolution; saves time by eliminating need for precycling prior to buffer equilibration
	Dry microgranular AIEC for similar performance characteristics as preswollen media after precycling; reduces possibility of bacterial growth
	Fibrous AIEC for maximum throughput at high flow rates
	Commonly used anion and cation exchange functional groups: DEAE (diethylaminoethyl tertiary amine) and CM (ether-linked carboxymethyl)
	Orthophosphate bifunctional cation exchanger for sharp separation of medium molecular weight molecules

Cellulose Powders

Whatman cellulose powders are used for Column and Thin Layer Chromatography (TLC).

Four high-purity cellulose powders are available using the partition mode.

CC31
Pure, microgranular cellulose powder for column separations.

CC41
Pure, binder-free microgranular cellulose powder for TLC.

CF1
Fibrous, long cellulose for batch separations.

CF11
Fibrous, medium cellulose powder for general column chromatography.

Ordering Information
Catalogue Number	Product	Description	Size
4020-050	CF1	Fibrous cellulose for batch separations	500 g
4021-050	CF11	Fibrous cellulose powder for column work	500 g
4021-500	CF11	Fibrous cellulose powder for column work	5 kg
4014-050	CC31	Microgranular cellulose powder for column work	500 g
4014-200	CC31	Microgranular cellulose powder for column work	2 kg
4061-050	CC41	Microgranular cellulose for TLC	500 g

Specialty Products for Protein Separations

CDR (Cell Debris Remover) Aids in protein purification through initial clean-up of cell lysates; removes unwanted suspended, colloidal and soluble matter, leaving target proteins in solutions.

Ordering Information
Catalogue Number	Product	Description	Size
4025-050	CDR	Cell Debris Remover	500 g
4025-200	CDR	Cell Debris Remover	2 kg

Anion exchangers
The weak anion exchangers are based on the diethylaminoethyl (DEAE) tertiary amine functional group. QA52 is a strongly basic anion exchange medium, containing quaternary amine groups.

DE23 (fibrous)
Allows fast flow rates especially after fines removal; suitable for negative charged biopolymers.

DE32 (dry microgranular)
Similar performance characteristics as DE52 after precycling.

DE51 (preswollen, microgranular)
Low overall net charge. For use with proteins bearing a high negative charge and for nucleic acids. Suitable for isocratic elution systems.

DE52 (preswollen, microgranular)
Probably the most widely used DEAE cellulose in the world; used for biopolymers with low to high negative charges; exhibits excellent resolution with good flow rates.

DE53 (preswollen, microgranular)
Partially quaternized DEAE anion exchanger, highly substituted and with higher capacity than DE52; can be used in series with DE51 and DE52 media.

QA52 (preswollen, microgranular)
A strongly basic, quaternary amine-bearing anion exchange medium, moderately substituted, with high protein capacity. Fully ionized, bears constant change under all pH conditions; excellent for high pH applications.

Typical Data - Anion Exchange Media
Physical Form	Functional Group	Normal pH Range	Small Ion Capacity	Protein Capacity1
			meq/dg*	mg/dg*	mg/mL Bed Volume
Dry Fibrous
DE23	Diethylaminoethyl	2–9.5	0.88–1.08	425b	60

Dry Microgranular
DE32	Diethylaminoethyl	2–9.5	0.88–1.08	700b	140

Preswollen Microgranular
DE51	Diethylaminoethyl	2–9	0.20–0.25	175a	30
DE52	Diethylaminoethyl	2–9.5	0.88–1.08	700b	130
DE53	Diethylaminoethyl	2–12	1.8–2.2	750b	150
QA52	Quaternary Ammonium	2–12	1.1	750b	150

*dg=dry gram
1 Protein capacity quoted:
a 0.005 M pH 8.5 phosphate buffer–Bovine Serum Albumin
b 0.01M pH 8.5 phosphate buffer–Bovine Serum Albumin

Ordering Information - Anion Exchangers DEAE and QA Celluloses
Catalogue Number	Product	Description	Size
4053-010	DE23	Fibrous DEAE cellulose	100 g
4053-025	DE23	Fibrous DEAE cellulose	250 g
4055-010	DE32	Dry microgranular DEAE cellulose	100 g
4057-050	DE52	Preswollen microgranular DEAE cellulose	500 g
4057-200	DE52	Preswollen microgranular DEAE cellulose	2 kg
4058-050	DE53	Preswollen microgranular DEAE cellulose	500 g
4058-200	DE53	Preswollen microgranular DEAE cellulose	2 kg
4065-050	QA52	Quaternary amine substituted	500 g

Cation exchangers
CM32 (dry microgranular)
High capacity medium for proteins, hormones, polypeptides and other biopolymers bearing low to high positive charges; excellent resolution with good column flow rates. Requires precycling to restore swelling. Equivalent to CM52 when swollen.

CM52 (preswollen, microgranular)
High capacity medium for proteins, hormones, polypeptides and other biopolymers bearing low to high positive charges; excellent resolution with good column flow rates.

P11 Cellulose Phosphate
Dry fiber. Recommended for column separations.

Typical Data - Cation Exchange Media
Physical Form	Functional Group	Normal pH Range	Small Ion Capacity	Protein Capacity1
			meq/dg*	mg/dg*	mg/mL Bed Volume
Preswollen Microgranular
CM52	Carboxymethyl	3–10	0.90–1.15	1180a	210
Dry Microgranular
CM32	Carboxymethyl	3–10	2.1–2.8	1180b	200
Dry Fibrous
P11	Orthophosphate	2–10	3.2–5.3	400–500	—

*dg=dry gram
1 Protein capacity quoted:
a 0.01M pH 5.0 acetate buffer–Lysozyme
b 0.01M pH 4.4 acetate buffer–Lysozyme

Ordering Information - Cation Exchangers CM Celluloses and P Phosphates
Catalogue Number	Product	Description	Size
4035-010	CM32	Dry microgranular carboxymethyl	100 g
4035-050	CM32	Dry microgranular carboxymethyl	500 g
4037-050	CM52	Preswollen microgranular CM cellulose	500 g
4037-200	CM52	Preswollen microgranular CM cellulose	2 kg
4071-010	P11	Dry bifunctional cation exchange cellulose	100 g
4071-050	P11	Dry bifunctional cation exchange cellulose	500 g
4071-200	P11	Dry bifunctional cation exchange cellulose	2 kg

Ion exchangers EXPRESS-ION® high flow rate media
EXPRESS-ION media are matrices whose flow characteristics have been greatly improved. The manufacturing process has been optimized so that the matrix retains its inherent property of fast kinetics of adsorption and desorption.

EXPRESS-ION D
A weak anion exchange cellulose substituted with diethylaminoethyl (DEAE) groups and recommended for separations between pH 2 and pH 9.

EXPRESS-ION Q
A strong anion exchange cellulose having general applicability in separations requiring an anion exchange step, with the benefit of wide pH versatility. The N,N,N-trimethyl hydroxypropyl amine (quaternary amine) group is fully ionized throughout pH range 2–12.

EXPRESS-ION C
A moderately acidic cation exchange cellulose. A weak ion exchanger recommended for separations between pH 4.5 and pH 10 to ensure the carboxymethyl functional group remains ionized.

EXPRESS-ION S
A strongly acidic cation exchange cellulose having general applicability in separations requiring a cation exchange step, with the benefit of wide pH versatility. The sulfoxyethyl functional group is fully ionized throughout the pH range 2–12.

Typical Data - Flow Rates (cm/h)
	Pressure (psi)
	5	7.5	10
EXPRESS-ION D	171.6	213.6	238.5
EXPRESS-ION Q	134.7	173.6	212.4
EXPRESS-ION C	94	127.2	165.3
EXPRESS-ION S	94	127.2	160.8

Column dimensions 45 cm ID x 15 cm.

Properties
	EXPRESS-ION D	EXPRESS-ION Q	EXPRESS-ION C	EXPRESS-ION S
Type	Weak Anion	Strong Anion	Weak Cation	Strong Cation
Working pH range	2–9	2–12	4.5–10	2–12
Typical Protein	—	—	—	—
Capacity mg/mL:
BSA	60	55	—	—
Lysozyme		—	162	153
Small Ion Capacity	—	1 meq/dg	—	—
Fiber Length	—	60–130 µm	—	—
Base Matrix	—	Microgranular cellulose	—	—
Typical Flow Rate	—	150 cm/hr	—	—
Physical Stability	—	Negligible volume changes	—	—
		due to ionic strength or pH

Ordering Information - EXPRESS-ION High Flow Rate Media
Catalogue Number	Description	Size
4079-0025	EXPRESS-ION D	250 g
4079-3025	EXPRESS-ION Q	250 g
4079-1025	EXPRESS-ION C	250 g
4079-2025	EXPRESS-ION S	250 g

Chromatography paper



> Chromatography paper

Whatman chromatography papers are the most widely used papers for chromatography worldwide. This acceptance and usage reflects the purity, high quality and consistency of Whatman papers. These qualities are relied upon by chromatographers and essential to successful reproducible chromatography. Whatman chromatography paper media are made from specially selected cotton cellulose. They are rigorously quality controlled for characteristics important to the chromatographer and to ensure uniformity within the grade. The chromatography paper product line includes standard cellulose and ion exchange grades.

Bulk silica media for column chromatography



> Bulk silica media for column chromatography

The Whatman bulk silica media is used for low pressure columns. Same base silica and chemistries as those used for Whatman TLC plates. Particularly useful for scaling up separations optimized by TLC. In addition, Whatman offers an all-purpose media for general column chromatography. Applicable for preparative low pressure column separations scaled up from Partisil analytical HPLC columns.

Features and Benefits

	High resolution
	Good flow characteristics
	High surface area

Ordering Information
Catalogue Number	Product	Particle Size (µm)	Other Specifications	Weight
Bulk Silica Media for Low Pressure Column Chromatography
4771-010	LPS-2 low pressure silica gel	37–53	Adsorption	450 m2/g; may be dry packed	1 kg
4776-001	LRP-2 ODS bonded silica gel	37–53	Reversed phase	LPS-2 silica bonded with 16% C; - may be dry packed; end-capped	100 g
4776-005	LRP-2 ODS bonded silica gel	37–53	Reversed phase	LPS-2 silica bonded with 16% C; - may be dry packed; end-capped	500 g

Ordering Information
Catalogue Number	Product	Particle Size (µm)	Other Specifications	Weight
Bulk Silica Media for General Column Chromatography
4132-100	ODS-3 (C-18)	32-75	Reversed Phase	Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load	100 g
4132-301	ODS-3 (C-18)	32-75	Reversed Phase	Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load	1 kg
4790-010	Silica, 60Å	230-400 mesh - (38-63)	Adsorption	Bulk media for column or - dry chromatography	1 kg
4790-050	Silica, 60Å	230-400 mesh - (38-63)	Adsorption	Bulk media for column or - dry chromatography	5 kg
4790-250	Silica, 60Å	230-400 mesh - (38-63)	Adsorption	Bulk media for column or - dry chromatography	25 kg
4791-005	Silica, 60Å	70-230 mesh - (63-212)	Adsorption	Bulk media for column or - dry chromatography	500 g
4791-010	Silica, 60Å	70-230 mesh - (63-212)	Adsorption	Bulk media for column or - dry chromatography	1 kg
4791-050	Silica, 60Å	70-230 mesh - (63-212)	Adsorption	Bulk media for column or - dry chromatography	5 kg
4791-250	Silica, 60Å	70-230 mesh - (63-212)	Adsorption	Bulk media for column or - dry chromatography	25 kg

NEW Purasil 60Å Silica Gel

Silica Gel Media for Flash Chromatography
Whatman Purasil high purity silica gel provides an excellent separation medium for flash chromatography purification of target molecules. Narrow particle size distribution and minimal fines enable fast separations with no loss of chromatographic performance.

Features and Benefits

	High resolution
	Excellent flow rates
	Direct scalability

Typical Data
Iron Content	<0.02%
Chloride Content	<0.10%
Loss on Drying	<7%
pH (10% suspension)	7 ± 0.5
Pore Volume	60–80 mL/g
Surface Area	500–600 m2/g

Ordering Information
Catalogue Number	Product	Dimensions
4745-010	230–400 mesh (38–63 µm)	1 kg
4745-250	230–400 mesh (38–63 µm)	25 kg
4746-010	70–230 mesh (63–210 µm)	1 kg
4746-250	70–230 mesh (63–210 µm)	25 kg

Thin layer chromatography (TLC)

Product innovations from Whatman have made Thin Layer Chromatography (TLC) a practical laboratory tool for both qualitative and quantitative analysis.

Features and Benefits

	Stringent quality standards assure a consistent level of resolution, accuracy and reproducibility
	Multiple samples and standards can be run simultaneously under identical conditions
	Wide range of chemistries and sizes to suit your application needs
	Sample preparation is simplified because plates are disposable
	Mobile phase need not be compatible with detector
	Available with or without fluorescent indicator

Linear-K: Fast, Accurate Spotting

Whatman pioneered the linear preadsorbent layer for easy, rapid and accurate sample application. The layer actually acts as a sponge to pre-concentrate the sample before it interacts with the silica layer. In order to facilitate sample application and the pre-concentrating power of the pre-adsorbent layer, Whatman made it thicker than the silica layer. This allows the analyst to apply sample in amounts never before attainable with standard TLC plates.

TLC Plates: Designations/Formats

Whatman has designed nomenclature as a simple and convenient way of distinguishing between the different types of plates.

The symbol for silica gel is K (for Kieselgel), followed by a qualifying number. K5: 10-12 µm silica, of pore size 150Å; K6: 10-12 µm silica, of pore size 60Å.

The high performance silica is prefixed by the letters HP: HP-K 4.5 µm silica, pore size 60Å.

Reversed phase plates, with a bonded alkyl group, are represented by a K followed by the length of the alkyl chain: KC-18 10-12 µm silica, 60Å, octadecyl bonded phase.

Additional format information is provided for each plate through the following letter codes:

L Preadsorbent Layer
This compresses each spot into a narrow horizontal band. Hence, it is known as Linear-K; prefix L.

D Channelled Plates
2 mm channels of clear glass separate each sample lane, preventing crossover. D indicates division.

F Fluorescent Indicator
Fluorescent plates glow bright green under Shortwave UV light. Samples which absorb shortwave UV at 254 nm are detected due to fluorescence quenching.

M Microscope Slide
Plate size 1" x 3".

P Preparative Layer
Has 500 µm or 1000 µm thickness for large sample sizes.

Using these letter codes it is easy to define any TLC plate, for example: PLK6DF = preparative K6 silica 60Å pore diameter featuring a channelled, fluorescent plate and the preadsorbent layer.

Typical Data
Type	Separation - Mode	Application	Layer - Thickness (µm)	Plate Size - (cm)	Fluorescent - Linear-K	Channeled	Indicator
C-18	Reversed Phase	General	200	1" x 3", 10 x 10,	Available	—	Available
			1000	5 x 20, 20 x 20
C-8	Reversed Phase	General	200	5 x 20, 20 x 20	—	—	Available
C-2	Reversed Phase	Small polar molecules	200	5 x 20, 20 x 20	—	—	Available
Diamond	Adsorption	General	250	2.5" x 7.5",	Available	Available	Available
				10 x 10, 5 x 20,10 x 20
Silica Gel (K6)	Adsorption	General;	250	1" x 3", 5 x 10,
	(60Å pore diameter)	untreated samples	500	5 x 20,10 x 20,	Available	Available	Available
			1000	20 x 20
Silica Gel (K5)	Adsorption	General;	250	5 x 10, 5 x 20,
	(150Å pore diameter)	untreated samples	500	20 x 20	Available	Available	Available
			1000
HPTLC (HP-K)	Adsorption	Small samples;	200	5 x 5, 10 x 10,	Available	Available	Available
	(4.5 µm particle size)	(nanograms and picograms)		10 x 20
Flexible	Adsorption	General anionic	250	20 x 20	—	—	Available
	Ion exchange	Anionic biopolymers	100	20 x 20

LabSciences

Bioxys, HIV p24, TNF a, IFN gamma, Agarose