|
| |
|
Advanced
Ion Exchange Cellulose
Whatman Advanced Ion Exchange Cellulose (AIEC) and Column
Chromatography (CC) products are ideal for separation of
biopolymers and suitable for a wide variety of applications.
Available as:
- Preswollen microgranular AIEC for high load capacity
fast kinetics and resolution; saves time by eliminating need
for precycling prior to buffer equilibration
- Dry microgranular AIEC for similar performance
characteristics as preswollen media after precycling;
reduces possibility of bacterial growth
- Fibrous AIEC for maximum throughput at high flow rates
- Commonly used anion and cation exchange functional
groups: DEAE (diethylaminoethyl tertiary amine) and CM
(ether-linked carboxymethyl)
- Orthophosphate bifunctional cation exchanger for sharp
separation of medium molecular weight molecules
Cellulose Powders
Whatman cellulose powders are used for Column and Thin Layer
Chromatography (TLC).
Four high-purity cellulose powders are available using the
partition mode.
CC31
Pure, microgranular cellulose powder for column separations.
CC41
Pure, binder-free microgranular cellulose powder for TLC.
CF1
Fibrous, long cellulose for batch separations.
CF11
Fibrous, medium cellulose powder for general column
chromatography.
| Ordering Information |
| Catalogue Number |
Product |
Description |
Size |
| 4020-050 |
CF1 |
Fibrous cellulose for batch
separations |
500 g |
| 4021-050 |
CF11 |
Fibrous cellulose powder for
column work |
500 g |
| 4021-500 |
CF11 |
Fibrous cellulose powder for
column work |
5 kg |
| 4014-050 |
CC31 |
Microgranular cellulose powder
for column work |
500 g |
| 4014-200 |
CC31 |
Microgranular cellulose powder
for column work |
2 kg |
| 4061-050 |
CC41 |
Microgranular cellulose for TLC |
500 g |
Specialty Products for Protein Separations
CDR (Cell Debris Remover) Aids in protein purification
through initial clean-up of cell lysates; removes unwanted
suspended, colloidal and soluble matter, leaving target proteins
in solutions.
| Ordering Information |
| Catalogue Number |
Product |
Description |
Size |
| 4025-050 |
CDR |
Cell Debris Remover |
500 g |
| 4025-200 |
CDR |
Cell Debris Remover |
2 kg |
|
|
|
Anion
exchangers
The weak anion exchangers are based on the diethylaminoethyl
(DEAE) tertiary amine functional group. QA52 is a strongly basic
anion exchange medium, containing quaternary amine groups.
DE23 (fibrous)
Allows fast flow rates especially after fines removal; suitable
for negative charged biopolymers.
DE32 (dry microgranular)
Similar performance characteristics as DE52 after precycling.
DE51 (preswollen, microgranular)
Low overall net charge. For use with proteins bearing a high
negative charge and for nucleic acids. Suitable for isocratic
elution systems.
DE52 (preswollen, microgranular)
Probably the most widely used DEAE cellulose in the world; used
for biopolymers with low to high negative charges; exhibits
excellent resolution with good flow rates.
DE53 (preswollen, microgranular)
Partially quaternized DEAE anion exchanger, highly substituted
and with higher capacity than DE52; can be used in series with
DE51 and DE52 media.
QA52 (preswollen, microgranular)
A strongly basic, quaternary amine-bearing anion exchange
medium, moderately substituted, with high protein capacity.
Fully ionized, bears constant change under all pH conditions;
excellent for high pH applications.
| Typical Data - Anion
Exchange Media |
| Physical Form |
Functional Group |
Normal pH Range |
Small Ion Capacity |
Protein Capacity1
|
| |
|
|
meq/dg* |
mg/dg* |
mg/mL Bed Volume |
| Dry Fibrous |
| DE23 |
Diethylaminoethyl |
2–9.5 |
0.88–1.08 |
425b |
60 |
| |
|
|
|
|
|
| Dry Microgranular |
| DE32 |
Diethylaminoethyl |
2–9.5 |
0.88–1.08 |
700b |
140 |
| |
|
|
|
|
|
| Preswollen
Microgranular |
| DE51 |
Diethylaminoethyl |
2–9 |
0.20–0.25 |
175a |
30 |
| DE52 |
Diethylaminoethyl |
2–9.5 |
0.88–1.08 |
700b |
130 |
| DE53 |
Diethylaminoethyl |
2–12 |
1.8–2.2 |
750b |
150 |
| QA52 |
Quaternary Ammonium |
2–12 |
1.1 |
750b |
150 |
*dg=dry gram
1 Protein capacity quoted:
a 0.005 M pH 8.5 phosphate buffer–Bovine Serum Albumin
b 0.01M pH 8.5 phosphate buffer–Bovine Serum Albumin
| Ordering Information
- Anion Exchangers DEAE and QA Celluloses |
| Catalogue Number |
Product |
Description |
Size |
| 4053-010 |
DE23 |
Fibrous DEAE cellulose |
100 g |
| 4053-025 |
DE23 |
Fibrous DEAE cellulose |
250 g |
| 4055-010 |
DE32 |
Dry microgranular DEAE cellulose |
100 g |
| 4057-050 |
DE52 |
Preswollen microgranular DEAE
cellulose |
500 g |
| 4057-200 |
DE52 |
Preswollen microgranular DEAE
cellulose |
2 kg |
| 4058-050 |
DE53 |
Preswollen microgranular DEAE
cellulose |
500 g |
| 4058-200 |
DE53 |
Preswollen microgranular DEAE
cellulose |
2 kg |
| 4065-050 |
QA52 |
Quaternary amine substituted |
500 g |
|
|
|
Cation
exchangers
CM32 (dry microgranular)
High capacity medium for proteins, hormones, polypeptides and
other biopolymers bearing low to high positive charges;
excellent resolution with good column flow rates. Requires
precycling to restore swelling. Equivalent to CM52 when swollen.
CM52 (preswollen, microgranular)
High capacity medium for proteins, hormones, polypeptides and
other biopolymers bearing low to high positive charges;
excellent resolution with good column flow rates.
P11 Cellulose Phosphate
Dry fiber. Recommended for column separations.
| Typical Data - Cation
Exchange Media |
| Physical Form |
Functional Group |
Normal pH Range |
Small Ion Capacity |
Protein Capacity1
|
| |
|
|
meq/dg* |
mg/dg* |
mg/mL Bed Volume |
| Preswollen
Microgranular |
| CM52 |
Carboxymethyl |
3–10 |
0.90–1.15 |
1180a |
210 |
| Dry Microgranular |
| CM32 |
Carboxymethyl |
3–10 |
2.1–2.8 |
1180b |
200 |
| Dry Fibrous |
| P11 |
Orthophosphate |
2–10 |
3.2–5.3 |
400–500 |
— |
*dg=dry gram
1 Protein capacity quoted:
a 0.01M pH 5.0 acetate buffer–Lysozyme
b 0.01M pH 4.4 acetate buffer–Lysozyme
| Ordering Information
- Cation Exchangers CM Celluloses and P Phosphates |
| Catalogue Number |
Product |
Description |
Size |
| 4035-010 |
CM32 |
Dry microgranular carboxymethyl |
100 g |
| 4035-050 |
CM32 |
Dry microgranular carboxymethyl |
500 g |
| 4037-050 |
CM52 |
Preswollen microgranular CM
cellulose |
500 g |
| 4037-200 |
CM52 |
Preswollen microgranular CM
cellulose |
2 kg |
| 4071-010 |
P11 |
Dry bifunctional cation exchange
cellulose |
100 g |
| 4071-050 |
P11 |
Dry bifunctional cation exchange
cellulose |
500 g |
| 4071-200 |
P11 |
Dry bifunctional cation
exchange cellulose |
2 kg |
|
|
|
Ion
exchangers EXPRESS-ION® high flow rate media
EXPRESS-ION media are matrices whose flow characteristics have
been greatly improved. The manufacturing process has been
optimized so that the matrix retains its inherent property of
fast kinetics of adsorption and desorption.
EXPRESS-ION D
A weak anion exchange cellulose substituted with
diethylaminoethyl (DEAE) groups and recommended for separations
between pH 2 and pH 9.
EXPRESS-ION Q
A strong anion exchange cellulose having general applicability
in separations requiring an anion exchange step, with the
benefit of wide pH versatility. The N,N,N-trimethyl
hydroxypropyl amine (quaternary amine) group is fully ionized
throughout pH range 2–12.
EXPRESS-ION C
A moderately acidic cation exchange cellulose. A weak ion
exchanger recommended for separations between pH 4.5 and pH 10
to ensure the carboxymethyl functional group remains ionized.
EXPRESS-ION S
A strongly acidic cation exchange cellulose having general
applicability in separations requiring a cation exchange step,
with the benefit of wide pH versatility. The sulfoxyethyl
functional group is fully ionized throughout the pH range 2–12.
| Typical Data - Flow
Rates (cm/h) |
| |
Pressure (psi) |
| |
5 |
7.5 |
10 |
| EXPRESS-ION D |
171.6 |
213.6 |
238.5 |
| EXPRESS-ION Q |
134.7 |
173.6 |
212.4 |
| EXPRESS-ION C |
94 |
127.2 |
165.3 |
| EXPRESS-ION S |
94 |
127.2 |
160.8 |
Column dimensions 45 cm ID x 15 cm.
|
Properties |
| |
EXPRESS-ION D |
EXPRESS-ION Q |
EXPRESS-ION C |
EXPRESS-ION S |
| Type |
Weak Anion |
Strong Anion |
Weak Cation |
Strong Cation |
| Working pH range |
2–9 |
2–12 |
4.5–10 |
2–12 |
| Typical Protein |
— |
— |
— |
— |
| Capacity mg/mL: |
|
|
|
|
| BSA |
60 |
55 |
— |
— |
| Lysozyme |
|
— |
162 |
153 |
| Small Ion Capacity |
— |
1 meq/dg |
— |
— |
| Fiber Length |
— |
60–130 µm |
— |
— |
| Base Matrix |
— |
Microgranular cellulose |
— |
— |
| Typical Flow Rate |
— |
150 cm/hr |
— |
— |
| Physical Stability |
— |
Negligible volume changes |
— |
— |
| |
|
due to ionic strength or pH |
|
|
| Ordering Information
- EXPRESS-ION High Flow Rate Media |
| Catalogue Number |
Description |
Size |
| 4079-0025 |
EXPRESS-ION D |
250 g |
| 4079-3025 |
EXPRESS-ION Q |
250 g |
| 4079-1025 |
EXPRESS-ION C |
250 g |
| 4079-2025 |
EXPRESS-ION S |
250 g |
|
|
| |
| |
Chromatography paper
Whatman chromatography papers are the most widely used papers for
chromatography worldwide. This acceptance and usage reflects the purity,
high quality and consistency of Whatman papers. These qualities are
relied upon by chromatographers and essential to successful reproducible
chromatography. Whatman chromatography paper media are made from
specially selected cotton cellulose. They are rigorously quality
controlled for characteristics important to the chromatographer and to
ensure uniformity within the grade. The chromatography paper product
line includes standard cellulose and ion exchange grades.
|
| |
| |
Bulk silica media
for column chromatography
 |
 |
|
| > Bulk silica media for
column chromatography |
|
The Whatman bulk silica media is used for low pressure columns. Same
base silica and chemistries as those used for Whatman TLC plates.
Particularly useful for scaling up separations optimized by TLC. In
addition, Whatman offers an all-purpose media for general column
chromatography. Applicable for preparative low pressure column
separations scaled up from Partisil analytical HPLC columns.
Features and Benefits
- High resolution
- Good flow characteristics
- High surface area
| Ordering Information |
| Catalogue Number |
Product |
Particle Size (µm) |
Other Specifications |
Weight |
| Bulk Silica Media for Low
Pressure Column Chromatography |
| 4771-010 |
LPS-2 low pressure silica gel |
37–53 |
Adsorption |
450 m2/g; may be dry packed |
1 kg |
| 4776-001 |
LRP-2 ODS bonded silica gel |
37–53 |
Reversed phase |
LPS-2 silica bonded with 16% C; - may be
dry packed; end-capped |
100 g |
| 4776-005 |
LRP-2 ODS bonded silica gel |
37–53 |
Reversed phase |
LPS-2 silica bonded with 16% C; - may
be dry packed; end-capped |
500 g |
| Ordering Information |
| Catalogue Number |
Product |
Particle Size (µm) |
Other Specifications |
Weight |
| Bulk Silica Media for
General Column Chromatography |
| 4132-100 |
ODS-3 (C-18) |
32-75 |
Reversed Phase |
Octadecyl-bonded; end-capped; -
polymeric; 10.5% carbon load |
100 g |
| 4132-301 |
ODS-3 (C-18) |
32-75 |
Reversed Phase |
Octadecyl-bonded; end-capped; -
polymeric; 10.5% carbon load |
1 kg |
| 4790-010 |
Silica, 60Å |
230-400 mesh - (38-63) |
Adsorption |
Bulk media for column or - dry
chromatography |
1 kg |
| 4790-050 |
Silica, 60Å |
230-400 mesh - (38-63) |
Adsorption |
Bulk media for column or - dry
chromatography |
5 kg |
| 4790-250 |
Silica, 60Å |
230-400 mesh - (38-63) |
Adsorption |
Bulk media for column or - dry
chromatography |
25 kg |
| 4791-005 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
500 g |
| 4791-010 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
1 kg |
| 4791-050 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
5 kg |
| 4791-250 |
Silica, 60Å |
70-230 mesh - (63-212) |
Adsorption |
Bulk media for column or - dry
chromatography |
25 kg |
NEW Purasil 60Å Silica Gel
Silica Gel Media for Flash Chromatography
Whatman Purasil high purity silica gel provides an excellent separation
medium for flash chromatography purification of target molecules. Narrow
particle size distribution and minimal fines enable fast separations
with no loss of chromatographic performance.
Features and Benefits
- High resolution
- Excellent flow rates
- Direct scalability
| Typical
Data |
| Iron Content |
<0.02% |
| Chloride Content |
<0.10% |
| Loss on Drying |
<7% |
| pH (10% suspension) |
7 ± 0.5 |
| Pore Volume |
60–80 mL/g |
| Surface Area |
500–600 m2/g |
| Ordering Information |
| Catalogue Number |
Product |
Dimensions |
| 4745-010 |
230–400 mesh (38–63 µm) |
1 kg |
| 4745-250 |
230–400 mesh (38–63 µm) |
25 kg |
| 4746-010 |
70–230 mesh (63–210 µm) |
1 kg |
| 4746-250 |
70–230 mesh (63–210 µm) |
25 kg |
|
| |
| |
Thin layer
chromatography (TLC)
Product innovations from Whatman have made Thin Layer Chromatography
(TLC) a practical laboratory tool for both qualitative and quantitative
analysis.
Features and Benefits
- Stringent quality standards assure a
consistent level of resolution, accuracy and reproducibility
- Multiple samples and standards can be run
simultaneously under identical conditions
- Wide range of chemistries and sizes to suit
your application needs
- Sample preparation is simplified because
plates are disposable
- Mobile phase need not be compatible with
detector
- Available with or without fluorescent
indicator
Linear-K: Fast, Accurate Spotting
Whatman pioneered the linear preadsorbent layer for easy, rapid and
accurate sample application. The layer actually acts as a sponge to
pre-concentrate the sample before it interacts with the silica layer. In
order to facilitate sample application and the pre-concentrating power
of the pre-adsorbent layer, Whatman made it thicker than the silica
layer. This allows the analyst to apply sample in amounts never before
attainable with standard TLC plates.
TLC Plates: Designations/Formats
Whatman has designed nomenclature as a simple and convenient way of
distinguishing between the different types of plates.
The symbol for silica gel is K (for Kieselgel), followed by a
qualifying number. K5: 10-12 µm silica, of pore size 150Å; K6: 10-12 µm
silica, of pore size 60Å.
The high performance silica is prefixed by the letters HP: HP-K 4.5
µm silica, pore size 60Å.
Reversed phase plates, with a bonded alkyl group, are represented by
a K followed by the length of the alkyl chain: KC-18 10-12 µm silica,
60Å, octadecyl bonded phase.
Additional format information is provided for each plate through the
following letter codes:
L Preadsorbent Layer
This compresses each spot into a narrow horizontal band. Hence, it is
known as Linear-K; prefix L.
D Channelled Plates
2 mm channels of clear glass separate each sample lane, preventing
crossover. D indicates division.
F Fluorescent Indicator
Fluorescent plates glow bright green under Shortwave UV light. Samples
which absorb shortwave UV at 254 nm are detected due to fluorescence
quenching.
M Microscope Slide
Plate size 1" x 3".
P Preparative Layer
Has 500 µm or 1000 µm thickness for large sample sizes.
Using these letter codes it is easy to define any TLC plate, for
example: PLK6DF = preparative K6 silica 60Å pore diameter featuring a
channelled, fluorescent plate and the preadsorbent layer.
| Typical Data |
| Type |
Separation - Mode |
Application |
Layer - Thickness (µm) |
Plate Size - (cm) |
Fluorescent - Linear-K |
Channeled |
Indicator |
| C-18 |
Reversed Phase |
General |
200 |
1" x 3", 10 x 10, |
Available |
— |
Available |
| |
|
|
1000 |
5 x 20, 20 x 20 |
|
|
|
| C-8 |
Reversed Phase |
General |
200 |
5 x 20, 20 x 20 |
— |
— |
Available |
| C-2 |
Reversed Phase |
Small polar molecules |
200 |
5 x 20, 20 x 20 |
— |
— |
Available |
| Diamond |
Adsorption |
General |
250 |
2.5" x 7.5", |
Available |
Available |
Available |
| |
|
|
|
10 x 10, 5 x 20,10 x 20 |
|
|
|
| Silica Gel (K6) |
Adsorption |
General; |
250 |
1" x 3", 5 x 10, |
|
|
|
| |
(60Å pore diameter) |
untreated samples |
500 |
5 x 20,10 x 20, |
Available |
Available |
Available |
| |
|
|
1000 |
20 x 20 |
|
|
|
| Silica Gel (K5) |
Adsorption |
General; |
250 |
5 x 10, 5 x 20, |
|
|
|
| |
(150Å pore diameter) |
untreated samples |
500 |
20 x 20 |
Available |
Available |
Available |
| |
|
|
1000 |
|
|
|
|
| HPTLC (HP-K) |
Adsorption |
Small samples; |
200 |
5 x 5, 10 x 10, |
Available |
Available |
Available |
| |
(4.5 µm particle size) |
(nanograms and picograms) |
|
10 x 20 |
|
|
|
| Flexible |
Adsorption |
General anionic |
250 |
20 x 20 |
— |
— |
Available |
| |
Ion exchange |
Anionic biopolymers |
100 |
20 x 20 |
|
|
|
LabSciences
|
