Kidney cancer
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   Category Human tissue array
   Cat. No. CL
   Product description Kidney cancer
   Data sheet CL1
CL1
   
   Images
  general_information
   No. of samples 59
   Text
Current version: CL1
Kidney cancer: 50 cores
Normal kidney: 9 cores

Price : 5 slide for 600 €

CL1 Human, kidney cancer
                   
  Age Sex Diagnosis Size(cm) Capsule Vein Other N M
1 52 F RCC, granular cell types 12 + - - NX M0
2 60 F RCC, clear cell type 6 + + - N0 M1
3 42 M RCC, clear cell type 9 + + - NX M0
4 59 M RCC, clear cell type 7,5 + - - NX M0
5 67 F RCC, clear cell and granular cell type 8 + - - NX M0
6 65 M RCC, granular and clear cell type 11 + - - NX M0
7 59 M RCC, clear cell and granular cell type 7 + + - NX M0
8 56 M RCC, clear and pseudosarcomatous type 7 + - - NX M1*
9 42 F RCC, clear cell type 10 - + - NX M0
10 50 M RCC, clear cell type 3,5 - - - N0 M0
11 52 M RCC, clear cell type 4 - - - N0 M0
12 68 F RCC, clear cell type 6 + + - N0 M0
13 60 F RCC, clear cell type 5 - + - N0 M0
14 69 F RCC, clear cell type 7,5 + - - NX M
15 54 M RCC, clear cell type 7 + - - NX M0
16 43 F RCC, clear cell type 4 + - - N0 M0
17 53 M RCC, clear cell type 5,5 - - +* NX M1**
18 . M RCC, clear cell type 3,5 - + - N0 M0
19 . . RCC, collecting duct type 4 + - - NX M0
20 40 M RCC, clear cell type 2,2 - - - NX M0
21 52 M RCC, clear cell type 6 + - - NX M0
22 63 F RCC, clear cell and granular cell type 7,5 + - - NX M0
23 64 M RCC, clear cell type 5,4 + - - NX M0
24 37 M RCC, collecting duct type 10,5 - - - N0 M0
25 65 M RCC, papillary type 5 - - - NX M0
26 74 M RCC, clear type 17 - + - N0 M0
27 67 M RCC, clear cell type 9 + + +*    NX M0
28 50 M RCC 9 - - - N0 M0
29 74 M RCC, clear cell type 17 - + - N0 M0
30 62 M RCC, clear granular cell 7 + + - NX M0
31 64 M RCC, papillary type 4 - - - NX M0
32 45 M RCC, clear cell type 6,5 - - - N0 M0
33 62 M RCC 10 + - - N1 M0
34 53 M RCC, clear cell type 4,5 + - - NX M0 
35 64 M RCC, clear cell type 6 - - - NX M0
36 72 M RCC, clear cell type 1,5 - - - NX M0
37 62 F RCC, clear cell type 11 - - +* NX M0
38 56 M RCC, clear cell type 4 + + - NX M0
39 58 M RCC 8,5 - - - NX M0
40 57 M RCC, clear cell and granular cell type 10 + - - N0 M0
41 64 F Transitional cell carcinoma, pelvis . . . . . .
42 43 F RCC, clear cell type 6 - - - NX M0
43 52 F RCC, granular cell type 7 - - - NX M0
44 64 M RCC, granular cell type 10 - + +* NX M0
45 57 M RCC, clear cell type 4,5 + - - NX M0
46 59 M RCC, clear cell type and granular cell type 8 + - - NX M0
47 47 M RCC, clear cell type 4 + - - NX M0
48 77 M RCC, clear cell type 2,5 + - - NX M0
49 47 F RCC, clear and granular cell type 8 + - - NX M0
50 55 F RCC, clear cell type 3 + - - NX M0
51                                Normal of No. 4
52                                Normal of No. 16
53                                Normal of No. 20
54                                Normal of No. 21
55                                Normal of No. 30
56                                Normal of No. 36
57                                Normal of No. 42
58                                Normal of No. 48
59                                Normal of No. 50
60                                Carbon particles
                   
#2 * lung metastasis              
#8 *: metastasis to contralateral kidney            
#17 *: perinephric fat involvement, **: bone metastasis            
#27 *: adrenal gland involvement, Gerota's fascia involvement            
#37 *: perinephric fat involvement            
#44 *: perinephric fat involvement            

General Information of the Tissue Array Slide :

Applications

  • 􀁺 Immunohistochemistry
  • 􀁺 In situ hybridization, RNA or DNA
  • 􀁺 Fluorescent in situ hybridization (FISH)
  • 􀁺 In situ PCR, RNA or DNA
  • 􀁺 TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • 􀁺 Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • 􀁺 Tissue array slides are shipped in room temperature.
  • 􀁺 If the slides are stored in 4 degree, they are good up to one year.

How processed

  • 􀁺 Tissues were initially fixed with formalin except for some of the animal tissues.
  • 􀁺 Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • 􀁺 Cleared by xylene, three changes for 1 hour each.
  • 􀁺 Infiltrated with 60°C paraffin, three changes for 1 hour each
  • 􀁺 Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • 􀁺 Dry slides for 1 hour in a oven at 60 degree.
  • 􀁺 Dewax slides in xylene for 4 minutes x 5 times
  • 􀁺 Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • 􀁺 Immerse slides in tap water for 5 minutes

Slide orientation

  • 􀁺 In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area
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