|
Product Name |
Rat
Thyroid Iodide Transporter (TIT) Antibodies |
|
Cat. #
TIT11-S |
Rabbit Anti-Rat TIT
Antibodies , neat serum, SIZE: 100 ul, Lot # 353334S; |
|
Cat. #
TIT11-A |
Rabbit
Anti-Mouse GALR3 (affinity pure), SIZE: 100 ug, Lot #
353334A; |
|
Cat. #
TIT11-P |
Rat TIT Control
peptide, SIZE: 100 ug, Lot # 1929P; |
|
Introduction |
Iodide (I-) is an essential
constituent of thyroid hormones. Thyroid epithelial cells for the
biosynthesis of thyroid hormones actively transport it. At the
cellular level, Iodide transport is mediated by intrinsic membrane
Na+/I+ symporter (NIS). Rat TIT (618 aa; ~66 kDa) is predicted to
contain 12 transmembrane domains. Both N and C-termini are predicted
to be cytoplasmic. |
|
Source of
Antigen and Antibodies |
A 16 AA peptide sequence (designated
TIT11-P; control peptide), near the C-terminal, cytoplasmic domain
of rat TIT (1) was synthesized, coupled to KLH, and antibodies
generated in rabbits. Control peptide was coupled Sepharose and
used as affinity support to purify antibodies. |
|
Form &
Storage |
Control peptide
Solution is provided in PBS, pH 7.4 at 1 mg/ml (100 ug/100 ul).
Antiserum is supplied as neat serum (100 ul soln or lyophilized).
Affinity pure antibodies were purified over the
peptide-Sepharose column and supplied as 1 mg/ml soln in PBS, pH
7.4 and 0.1% BSA as stabilizer (100 ul in solution or
Lyophilized). The peptides and antibodies also contain 0.1%
sodium azide as preservative. Lyophilized products should
be reconstituted in 100 ul water and gently mixed for 15 min at
room temp. All peptide/antibody received in solution or
reconstituted from lyophilized vials should be stored frozen at
-20oC or below in suitable aliquots. It is not recommended to
store diluted solutions. Avoid freeze and thaw. |
|
Recommended Usage |
|
ELISA |
Control peptide can be
used to coat ELISA plates at 1 ug/ml and detected with
antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for
affinity pure). |
|
Western Blot |
Western Blotting
(1:1K-5K for neat serum and 1-10 ug/ml for affinity pure
using Chemiluminescence technique). An antibody raised
against this epitope has been shown to recognize a ~80-90
kDa glycosylated and ~55 kDa non glycosylated protein in
rat thyroid cell line FRTL-5 (2) band in rat and human
brain (2). Deglycosylation reduces the mol. Wt to approx.
53 kDa (2). |
|
Histochemistry &
Immunofluorescence |
we recommend the
use of affinity purified antibody at 2-20 ug/ml in
formaldehyde fixed tissue (2). |
|
Specificity &
Cross-reactivity |
The 16-aa rat
TIT11-P peptide sequence is unique to TIT and no
significant sequence is seen with glucose transporter
or other Na+-dependent transporters. TIT from other
species has not been cloned. Actual cross-reactivity
of antibodies in various species has not been studied. |
|
References |
1. Dai G et al
(1996) Nature 379, 458-460
2. Paire A et al (1997) J Biol. Chem. 272,
18246-18249 |
|
|
*This product
is for In vitro research use only. |
