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Bone Analysis

25-09-2013

Preparation of Hard Tissue Specimens 1. Preparation of bone tissue specimens To acquire various information from tissues and cells in bone, gross morphological examinations and histomorphometrical analysis are performed with bone tissue...

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Antibody Production

25-09-2013

Custom peptide polyclonal antisera have become an instrument of paramount importance in the life science tool-box. However, raising peptide polyclonal antibodies is a laborious process and for a successful outcome,...

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Comparison

25-09-2013

Comparison of posttranslational modifications of a protein The primary structure of the protein must be known!Samples containing a given protein either obtained after expression in different expression systems (e.g. prokaryotic vs....

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posttranslational

25-09-2013

  Identification of posttranslational protein modifications  The primary structure of the protein must be known! You provide us with: a) or b): a) Homogenous protein in an excised band from a dried SDS-gel (stained with...

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Identification

25-09-2013

Storage Logistics You provide us with a,b, or c: a) Homogenous protein in an excised band from a dried SDS-gel (stained with Coomassie Blue or Silver, preferably without fixation). Please enclose a gel...

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Molecular weight

25-09-2013

MALDI-TOF Determination of the absolute molecular weight of the protein subunit(s) You provide us with: At least 10 pmol purified native protein (for subunit molecular weigths up to 30 kDA) or 20...

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MALDI TOF

25-09-2013

  Cutting out of Bands or Spots of Interest We offer a MALDI TOF mass analysis and protein identification service together with our partner Mobidab GmbH & Co. KG in Leipzig. All...

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Quantitative

25-09-2013

Comparative Quantitative Analysis of Electrophoresis Results The 1 or 2 D gel images will be processed and recorded in TIF format. The TIF files are analysed by the Phoretix analysis software...

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Comparative 1D or 2D

25-09-2013

Comparative 1D or 2D Protein Gel Electrophoresis Comparative 1 D SDS-PAGE Electrophoresis: Appropriate solubilized protein amounts will be seperated on SDS PAGE gels (Percentages between 6 and 20% are available) and stained...

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Sample Preparation

25-09-2013

Optimal sample preparation is crucial for any proteome analysis. The following parameters define the quality of each proteomics approach:  Solubilization protocol: Our partner Fraunhofer IGB can either apply an in house...

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