The Grandient solution!

"Son of Metrizamide"

Nycodenz Solution!(Nycoprep Universal 60%)

Sterile Ready-Made 60% (w/v) Solution in Water

Endoxin-Tested

Makes Density Gradient Solution: Simple

                                                                     Reproductible

May be Sterilized by Autoclaving

     

DATA SHEET

                                                                                           Nycodenz

1.                                   CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

Product name:               Nycodenz

Application:                   Isolation of biological particles using centrifugation techniques

2.                                   COMPOSITION, INFORMATION ON INGREDIENTS

Composition:               Main ingredients:      CAS No.:          Content:      Hazard classification:

Off-white powder         66108-95-0                           None

3.                                   HAZARDS IDENTIFICATION

Emergency overview:    Powder. No hazard if spilled

Health Hazard:              .Non-toxic

4.                                   FIRST-AID MEASURES

General:                        Miscible with water.

Eyes:                              Flush eyes with water. If irritation occurs, seak medical advice.

Skin contact:                 Wash with  water.

Ingestion:                      Wash mouth thoroughly with water and drink water if substance has been swallowed, drink large quantities of water.

Inhalation:                     Not applicable.

5.                                   FIRE-FIGHTING MEASURES

Extinguishing media:    Use extinguishing media appropriate for surrounding fire.

Flammable properties:  Not flammable. This product does not present any fire or explosion hazard.

6.                                   ACCIDENTAL RELEASE MEASURES

Personal precautions:   Wear gloves, other protection is not necessary

Cleanup procedures:     Clean up, contaminated area should be washed with soap and water.

7.                                   HANDLING AND STORAGE

Handling precautions:   Should be handled as an unknown chemical.

Storage precautions:     Store in original container according to temperature stated on container and in package insert

8.                                   EXPOSURE CONTROLS, PERSONAL PROTECTION

Ventilation:                    No particular ventilation requirements.

Protective gloves:          Hand protection is recommended.

Eye protection:              Eye protection not normally required.

Protective clothing:       Protective clothing is not necessary.

9.                                   PHYSICAL AND CHEMICAL PROPERTIES

Apperance:                    Powder

Colour:                          Off-white

Solubility:                      Readily miscible with water.

Spesific gravity:             2.1 g/ml                    

pH-value:                       5.0-7.0

10.                                 STABILITY AND REACTIVITY

Stability:                        Stable and not reactive

11.                                 TOXICOLOGICAL INFORMATION

Acute Effects:                 None

Skin Effects:                   None

Eye Effects:                    None

12.                                 ECOLOGICAL INFORMATION

Summary:                      This product is readily miscible with water and rated non- toxic. It has no potential for bioaccumulation.

13.                                 DISPOSAL CONSIDERATION

General information:     Disposal should be made in accordance with existing disposal practices employed for biological material

14.                                 TRANSPORT INFORMATION

General information:     Transportation of the product is not regulated by ADR. Fragile containers, handle with care. Protect from high temperatures.

15.                                 REGULATORY INFORMATION

EU regulations: Not regulated under the European Community Directive 93/21/EEC.

16.                                 OTHER INFORMATION

Revision Date:               October 2000

Replaces:                      January 1992

This Safety Data Sheet complies to the European Community Directive 93/112/EC amended 91/155/EEC.

 

Anti-MAGE(IN)                               CATALOG No. 28004                                         299 USD /100ul

BACKGROUND:


The melanoma-associated antigen (MAGE) gene family includes at least 17 related genes, namely MAGE-A1 to A12, MAGE-B1 to B4 and MAGE-C1 (1) The MAGE genes are expressed by tumors of various histological types, but they are silent in normal cells, with the exception of male germ-line cells. Thus the antigens encoded by MAGE-A, -B, -C genes should be strictly tumor specific. Because the MAGE antigens are shared by many tumors and on account of their strict tumor specificity, they are of particular interest for cancer immunotherapy (2).


SOURCE:


Rabbit anti-MAGE polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 245-259 of human MAGE-1(QEKYLEYRQVPDSDP). There are two amino acids different from mouse.


REACTIVITY:


This epitope affinity purified rabbit polyclonal antibody is specific for MAGE-1. The antibodies were evaluated for specificity by immunohistostaining. A positive staining is observed in human Melanoma.


APPLICATION:


The working concentrations for specific applications should be determined by the investigator. The results might be affected by several factors, including secondary antibody
affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations.
ELISA: 0.1-1.0 µg/ml.
Western blot: 0.5 to 2 µg/ml.
IP: 3.0-5.0 µg/extract from 107 cells.
IHC: 1:100


STORAGE:


This polyclonal antibody is supplied as an epitope affinity purified rabbit IgG, 100 µg in 200 µl of phosphate buffered saline (pH 7.4) containing 0.02% sodium azide. Store at 2-8 °C for up to one month. Store at –20 oC for long-term storage. Avoid repeated freezing and thawing.


REFERENCES:


1. Gillespie AM et al (1999) Cancer Treat. Rev. 25, 219-227.
2. Chames P et al (2000) Proc. Natl. Acad. Sci. USA 97(14), 7969-7974.
Human melanoma stained with anti-MAGE (Cat# 28004) by IHC Histain Kit-AEC (Cat# 29702). The formalin-fixed tissues was boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling @ RT for 20 min, then probed at a dilution 1:100 for 10 min at room temperature.

Dendritic Cell Therapy

   
04-huIL1B-50 µg human IL-1B, 50 µg 50µg 195 $
04-GMPhuIL4-50 µg GMP human IL-4, 50 µg 50µg 399 $
04-RESIL4-100µg Research human IL-4, 100 µg 100ug 245 $
04-RESIL4-50µg Research human IL-4, 50 µg 50µg 195 $
04-huIL-6-50µg human IL-6, 50 µg 50µg 195 $
04-huIL-10-50µg human IL-10, 50 µg 50µg 195 $
04-huIL-11-1mg human IL-11, 1mg 1mg 95 $
04-huIL-11-5mg human IL-11, 5mg 5mg 195 $
04-huIL-15-50µg human IL-15, 50 µg 50µg 195 $
04-RHUGM-CSF-300UG mGMP GM-CSF, 300 µg 300µg 385 $
04-RHUGM-CSF-50UG mGMP GM-CSF, 50 µg 50µg 90 $
04-HGH-4IU HGH Lyophilisd powder 4IU 95 $
04-SOD-50µg human SOD , 500 µg 500ug 195 $
04-RHUTNFA-50 µg human TNF-a 50µg 399 $
04-GMPTAA- 10µg GMP TAA’s Tumor Associated Antigens 10 µg 199 $
Different subpopulation of DCs can be obtained by separating and cultivating their precursors (for example monocytes) under different conditions. The most common subpopulation is obtained by using a combination of various cytokines, including interleukin 4 (IL-4) and granulocyte-monocyte colony-stimulating factor (GM-CSF)

In order to supply researchers and clinicians using DC with necessary tools of the trade, BruCells is developing preparations of these reagents suitable for the subsequent application of the DC to patients, i.e manufactured in accordance with Good Manufacturing Practice (GMP) and fulfilling state of the art specifications.

Cytokines are a class of proteins, which are secreted by living cells. They have several functions, such as promoting cellular growth, activate cells or destroy target cells such as cancer cells. A subgroup called Interleukins (IL) function as messengers between leukocytes. Cytokines have many potential applications. The demand is expected to grow in the future with the development of cellular therapies.

In the case of cellular immunotherapy of cancer, cytokines are mainly used for the maturation of DCs. For the moment, the market consists mainly of the many research and development groups which are working on therapeutic vaccines and which need to obtain reliable sources of clinical grade cytokines.

The production and marketing of clinical grade (GMP) human recombinant cytokines by BruCells present several advantages for the company:

   
- these products enter the production of DC vaccines and the availability of GMP materials at
  competitive prices is a competitive advantage
- the marketing of such products may help to establish earlier a presence in the market
- the contribution margin may help to fund the development of vaccines


Starting in September, 2003, clinical grade recombinant human Interleukin-4 can be supplied in vials containing 50 micrograms of lyophilized IL4.

Starting in August, 2004, clinical grade recombinant human Granulocyte-Monocyte Colony-Stimulating Factor can be supplied in vials containing 50 micrograms of lyophilized GM-CSF.

Research grade cytokines for experimental use in vitro can be provided subject to availability : IL-1b, IL-4, IL-6 and others. Please enquire.

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