IL-12
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Specification sheet

Recombinant Interleukin 12 Human (IL-12)

Catalog No: PC108-1 (2 micrograms) 180€
Catalog No: PC108-2 (10 micrograms) 285€
Catalog No: PC108-3 (1 mg) CALL/EMAIL
Source CHO cells (Chinese Hamster Ovarian cells)
Formulation

The sterile filtered solution was lyophilized from 0.5x PBS, pH 7.2.

Reconstitution We recommend a quick spin followed by reconstitution in 1x PBS, pH 7.2-
7.4 to a concentration of 0.2 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 degree Celsius for 1 week or -20 degree Celsius for future use.
Storage The lyophilized protein is stable for a few weeks at room temperature, but
best stored at -20 degree Celsius. Reconstituted IL-12 should be stored in working aliquots at -20 degree Celsius.
Purity Greater than 95% by SDS-PAGE and HPLC analyses.
Biological Activity The ED50 as determined by the stimulation of IFN-γ production by mouse
splenocytes co-stimulated with IL-12 is <= 0.1 ng/mL, corresponding to a specific activity of >= 1 x 10^7 units/mg.
Description Interleukin-12 is a regulatory protein produced by activated B-lymphocytes
and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+T cells and NK cells. Human IL-12 also promotes the development of proinflammatory/ Th1- like CD4+ cells and cytotoxic CD8+T cells. Human IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
Endotoxin Endotoxin level is less than 0.1 ng/μg (1EU/μg).