|
Protocol ( For Research
Use Only ) |
|
1. Dispense
100µl
of the standards,
controls, and samples into their designated wells. |
| 2. Seal
the plate and incubate for 2hours at the room temperature.
|
 |
| 3. Wash
the plate 3 times with Wash buffer. |
| 4. Add
100µl of the diluted Biotinylated- antibody
to each well. |
| 5. Seal
the plate and incubate it for 2hours at the room temperature.
|
| 6. Wash
the plate 3 times with wash buffer. |
| 7. Add
100µl of the diluted Streptavidin-HRP conjugate to each well.
|
| 8. Seal
the plate and incubate it for 30min at the room temperature.
|
| 9. Wash
the plate more than 4 times with wash buffer. |
|
10. Immediately dispense 100µl
TMB substrate solution (TMB A + TMB B) into each well and
incubate at room
temperature for 15-30mins. |
|
11. Add 50µl of Stop Solution to each well. |
|
12. Read absorbance with a microplate reader at
450nm. |
