Human IL-6 ELISA Kit
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Specification sheet

High Sensitivity Human IL-6 ELISA Kit

Catalog No: PL103-S1 One plate; 96 well determinations 445€
Catalog No: PL103-B2 Two plates; 192 well determinations 705€
Application

The IL-6 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. The antigen and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. Revelation step includes Streptavidin-Horse Radish peroxidase and TMB as chromogen.

Sensitivity Less than 0.8 pg/mL Range of Detection 1.56 pg/mL-50pg/mL
Specificity No cross reactivity with other cytokines
Sample Volume: 100μl Calibration: WHO 89/548

Linearity of Dilution:

A human serum pool containing 50 pg/ml of measured IL-6 was serially diluted in standard buffer diluents over the range of the assay. Linear regression of samples versus the expected concentration yielded a correlation coefficient of 0.99.
Recovery:
Recovery of IL-6 added to pooled normal serum is 81.6% (72 % to 100%) for IL-6
concentrations ranging from 50 to 1.5 pg/ml