HTGF beta-1 recombinant
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Product Details: Human transforming growth factor beta-1 (TGF beta-1) recombinant

Basic Information

 
Product ID: GF-230-2
Product Name: Human transforming growth factor beta-1 (TGF beta-1) recombinant
Unit: 5µg
Price: 365 Euro
Availability: Available
Category: Growth Factors, Cytokines, Receptors and their Antibodies
Subcategory: Growth Factors/Cytokines/Enzymes

 

Product Details

 
Description: Human Transforming Growth Factor beta1 (TGF- beta1 ) is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. Mature TGF- beta1 is a disulfide-linked homodimer of two chains composed of 112 amino acid residues each. There is a 70% homology between human TGF- beta1 and human TGF- beta2.

Human TGF- beta1 is produced in genetically engineered bacteria, and purified by sequential chromatography.

 
Molecular Weight: 25 KDa (dimeric protein)
Biological Activity: The biological activity of human TGF-&# beta1 is determined by measuring the acidification of the culture medium (Rat-1 cells) exposed to the growth factor.

 
Formulation: 25 µl (0.20 mg/ml) in 5 mM HCl (pH 2.5). Some of the liquid in the vial could have been evaporated with changes in the final volume. However, the 5µg of the protein are still inside the vial. We recommend, in order to control the proper concentration, to dilute the entire content to a known volume with 5mM HCl. Do not freeze this material.
Purity: Over 98% pure by non-reduced SDS gel electrophoresis.
Storage Conditions: Store at +4°C.
Stability: Several months at +4°C.
Temperature: +4°C
References: 1. Ichijo, H. et al.(1994) The Journal of Biological Chemistry, Vol 266, No. 33, May, 22459-22464
2. Yan, Z., Winawer, S., and Friedman, E., (1994) The Journal of Biological Chemistry, Vol 269, No. 18, May, 13231-13237.