HPLC
Home Up

 

 

High performance liquid chromatography (HPLC)

Whatman offers an extensive product line of High Performance Liquid Chromatography (HPLC) products ranging from the Partisil® high purity silica gel for normal phase separations to seven different bonded phases for reversed phase or ion exchange separations. In addition, the Partisphere®, 5µm spherical media is available with different chemistries in a void sealing column configuration for optimum speed and resolution.

Application specific HPLC columns

TAC 1*
For great discoveries such as Taxol®, Whatman technology optimally separates the closely eluting Taxanes of Pacific yew trees.

Whatman worked closely with two leading customers to develop a specific bonded phase that achieves baseline resolution of the paclitaxel molecule from its closest impurity. Each lot of TAC 1 (Taxane Analysis Column) is tested with a paclitaxel chromatographic purity separation to ensure the best possible reproducibility.

*Richheimer SL et al. Anal Chem. 1992; 64:2323-2326

MAX-1
For use in specialized separation for corn and soy protein.

Ordering Information - Application Specific HPLC Columns
Catalogue Number Product Particle Size (µm) Dimensions (mm) Quantity/Pack
4601-1001 TAC 1 5 4.6 x 250 1
4120-001 MAX-1 5 4.6 x 250 1
 

 

Partisil® High performance liquid chromatography (HPLC)columns
 

 

High performance liquid chromatography (HPLC)
>  (HPLC)columns
Partisil® High performance liquid chromatography (HPLC)columns
Whatman offers a wide range of high quality columns to meet your specific needs. In addition to the innovative Whatman Void Sealing Columns, Whatman makes available a selection of standard end fitting column configurations for your analytical and preparative needs. They are specifically designed for compatibility with all HPLC instrumentation. 

 

The Whatman Partisil® is a high purity irregular silica gel available in both 5 µm and 10 µm particle sizes with a pore size of 80Å. The choice of column packing includes Silica, C-18 polymeric phases (ODS-3,ODS-2) and C-8. Also available are SAX (Strong Anion Exchanger), SCX (Strong Cation Exchanger) and PAC (Polar Amino Cyano). These columns provide reproducible results, column to column, lot to lot.

Due to the greater surface area of the irregular Partisil, the medium offers enhanced selectivity and loading capacity. Through uniform particle sizing, back pressure is minimized. Also, the neutral pH of Partisil provides for better peak symmetry without the need for mobile phase modifiers.

Standard Analytical
4.6 mm ID x 25 cm long, standard analytical column for research, methods development and routine separations. After optimization, other sizes can be considered for greater speed or capacity. Allows direct scale-up or scale-down to other size columns. Supplied with Whatman compression screw end fittings.

RAC II
4.6 mm ID x 10 cm long. Second-generation Rapid Analysis Chromatography for faster analytical separations and reduced solvent consumption. Operates at low back pressure, even at high flow rates, prolonging column life. Connects easily to most LC instrumentation with convenient Whatman compression screw end fittings.

Magnum 9 (50 cm)
9.4 mm ID x 50 cm long. Semi preparative columns for microgram to gram quantities. Unique coned outlet allows high load capacity with minimal peak distortion. Durable construction ensures extended service. Magnum 9 columns are compatible with today's HPLC instruments, allowing you to use the same equipment for analytical and preparative work.

Magnum 9 (25 cm)
9.4 mm ID x 25 cm long.

Magnum 20 (50 cm)
22 mm ID x 50 cm long. Preparative columns for multigram separations. Coned outlet allows high load capacity with minimal peak distortion. Durable construction ensures extended service. Magnum 20 columns provide sufficient yield and resolving power to accomplish difficult separations on a single pass, achieving high product purity.

Magnum 20 (25 cm)
22 mm ID x 25 cm long.

Ordering Information
- Partisil High Performance Liquid Chromatography (HPLC) Columns
(with WCS Standard End-Fittings)
Catalogue Number Column Configuration Particle Size (µm) Dimensions
Bonded Phase
Partisil Silica
4215-001 Standard analytical 5 4.6 x 250 mm
4216-001 Standard analytical 10 4.6 x 250 mm
4230-120 Magnum 9 10 9.4 x 250 mm
4230-220 Magnum 9 10 9.4 x 500 mm
4232-220 Magnum 20 10 22 x 500 mm
Partisil ODS-3
4222-225 RAC II 5 4.6 x 100 mm
4238-001 RAC II 5 4.6 x 250 mm
4228-001 RAC II 10 4.6 x 250 mm
4230-125 Magnum 9 10 9.4 x 250 mm
Partisil C-8
4222-232 RAC II 5 4.6 x 100 mm
4239-001 Standard analytical 5 4.6 x 250 mm
4229-001 Standard analytical 10 4.6 x 250 mm
4232-132 Magnum 20 10 22 x 250 mm
Partisil SAX
4222-227 RAC II 5 4.6 x 100 mm
4226-001 Standard analytical 10 4.6 x 250 mm
4250-001* Standard analytical 10 4.6 x 250 mm
(with Solvecon)
Partisil SCX
4222-228 RAC II 5 4.6 x 100 mm
4227-001 Standard analytical 10 4.6 x 250 mm
4251-001* Standard analytical 10 4.6 x 250 mm
Partisil PAC
4235-001 Standarad analytical 5 4.6 x 250 mm
4225-001 Standarad analytical 10 4.6 x 250 mm
4232-226 Magnum 20 10 22 x 500 mm
Partisil 10 ODS
4223-001 Standard analytical 10 4.6 x 250 mm
Partisil 10 ODS-2
4224-001 Standard analytical 10 4.6 x 250 mm
4230-124 Magnum 9 10 9.4 x 250 mm
Accessories
4334-225 Frits, 1/4" diameter, 2 µm porosity, 10/pack
 

 
 

Whatman void sealing (WVS) columns

High performance liquid chromatography (HPLC)
Whatman void sealing (WVS) columns
Whatman WVS columns are renowned for their high quality, innovative design and exceptional durability. 

 

 

Features and Benefits

  • Void sealing columns can last twice as long as standard end fitting columns, saving as much as 50% on cost per test
  • Available packed with spherical and irregular media
  • Integral void sealing mechanism prolongs column life
  • Reusable, hand tightened end-fittings save money, allow for wrench-less installation and rapid column changes
  • Require no holder or module, meaning fewer components, reduced cost

Optimum Resolution

  • Typical column efficiencies for:
  • Partisil 10 µm media-45,000 N/m
  • Partisil 5 µm media-65,000 N/m
  • PartiSphere 5 µm media-90,000 N/m

Partisil® Irregular Media
Available in prepacked, replaceable columns and a choice of 5 µm and 10 µm phases. These include Silica, our popular ODS-3 and the other reversed phase packings ODS-2 and C-8. Also available are SAX (Strong Anion Exchanger), SCX (Strong Cation Exchanger) and PAC (Polar Amino Cyano).

Due to the greater surface area of the irregular Partisil, the medium offers enhanced selectivity and loading capacity. Through uniform particle sizing, back pressure is minimized. Also, the neutral pH of Partisil provides for better peak symmetry without the need for mobile phase modifiers.

PartiSphere® Spherical Media
Available in prepacked columns and a choice of 5 µm high performance phases. In addition to its efficient pure silica and monomeric C-18 and C-8, Whatman has added WCX (Weak Cation Exchanger) as well as SAX, SCX and PAC. PartiSphere media feature narrow particle size distribution and excellent reproducibility.

PartiSphere WVS Columns: Engineered to Provide Unsurpassed Consistency and Longevity

PartiSphere RTF
PartiSphere RTF (Reduced Tailing Factor) HPLC columns are base-deactivated columns. They employ a unique proprietary process that effectively "deactivates" the secondary chromatographic effect due to residual silanols. In addition, these columns are extremely stable and can be used from pH 2 to pH 8 with no loss in performance. Excellent for separation of basic compounds without the need for amine-modified mobile phases. PartiSphere RTF is available prepacked in Whatman Void Sealing (WVS) and Analytical (WCS) column configurations and in a choice of C-18, C-8 and cyano phases.

Features and Benefits

  • All PartiSphere columns are guaranteed to perform reproducibly every time, thanks to multiple quality control tests for both primary and secondary separation mechanisms
  • Polished internal column walls ensure packing symmetries and efficiencies
  • PartiSphere RTF (Reduced Tailing Factor) employs a unique proprietary process that effectively "deactivates" the secondary chromatographic effect due to residual silanols

HPLC Guard Cartridge System

High performance liquid chromatography (HPLC)

HPLC Guard Cartridge System

The prepacked, disposable plastic guard cartridge retains unwanted materials that can harm your analytical column. Used in a wide range of applications, the HPLC guard cartridge system offers high efficiency, convenience and cost savings. The guard cartridge holder is available in two configurations. The integral system attaches directly to and becomes an integral part of the WVS replacement column requiring no connecting tubing. Because of this, there is virtually no loss in efficiency.

The universal system can be used with any standard analytical column, bringing guard cartridge convenience to traditional HPLC columns. Guard cartridges are available in four phases: silica, reversed phase, anion exchanger and cation exchanger.

Zero-Dead-Volume
Guard columns offer protection by trapping unwanted compounds that would otherwise be strongly retained on the HPLC column. One of the problems inherent with other guard columns is that they either contribute too much dead volume or add to the analysis times of the separation (especially in short, high speed columns). Whatman has overcome this by developing a zero-dead-volume HPLC guard cartridge system which eliminates almost all extra void volume and does not detrimentally affect the separation.

Typical Data
Partisil Bonded Phase Other Specifications
Silica Irregular. Pore Size 85Å
ODS-3 10.5% carbon load; end capped; polymeric
ODS-2 16% carbon load; polymeric
C-8 8.5% carbon load; end capped; monomeric brush
PAC 0.85% N
SAX 0.85% -NR5+
SCX 0.40% S
PartiSphere Bonded Phase
Silica Spherical. Pore Size 120Å
C-18 10% carbon load; end capped; brush
C-8 6% carbon load; end capped; brush
PAC 0.85% N
SAX 0.8% -NR3+
SCX 0.40% S
PartiSphere RTF Bonded Phase
C-18 22% carbon load; monomeric brush
Ordering Information- WVS Columns
Catalogue Number Particle Size (µm) Column Type Column Size (mm)
Partisil1 5 µm & 10 µm Columns Only*
Partisil 5 Silica
4681-1501 5 Whatman Void Sealing 4.6 x 250
Partisil 5 ODS-3
4681-0502 5 Whatman Void Sealing 4.6 x 125
4681-1502 5 Whatman Void Sealing 4.6 x 250
Partisil 5 SAX
4681-0505 5 Whatman Void Sealing 4.6 x 125
4681-1505 5 Whatman Void Sealing 4.6 x 250
Partisil 5 SCX
4681-1507 5 Whatman Void Sealing 4.6 x 250
Partisil 5 ODS-2
4681-1509 5 Whatman Void Sealing 4.6 x 250
Partisil 10 ODS-3
4682-1502 10 Whatman Void Sealing 4.6 x 250
Partisil 10 SAX
4682-1505 10 Whatman Void Sealing 4.6 x 250
Partisil 10 SCX
4682-1507 10 Whatman Void Sealing 4.6 x 250
PartiSphere2 5 µm Columns*
PartiSphere Silica
4621-0501 5 Whatman Void Sealing 4.6 x 125
4621-1501 5 Whatman Void Sealing 4.6 x 250
PartiSphere C-18
4621-0502 5 Whatman Void Sealing 4.6 x 125
4621-1502 5 Whatman Void Sealing 4.6 x 250
PartiSphere C-8
4621-0503 5 Whatman Void Sealing 4.6 x 125
PartiSphere SAX
4621-0505 5 Whatman Void Sealing 4.6 x 125
4621-1505 5 Whatman Void Sealing 4.6 x 250
PartiSphere SCX
4621-0507 5 Whatman Void Sealing 4.6 x 125
4621-1507 5 Whatman Void Sealing 4.6 x 250
PartiSphere PAC
4621-0508 5 Whatman Void Sealing 4.6 x 125
4621-1508 5 Whatman Void Sealing 4.6 x 250
Whatman Base-Deactivated (WCS) HPLC Columns - PartiSphere2 5 µm RTF (Reduced Tailing Factor)
PartiSphere RTF C-18
4522-0102 5 Standard Analytical 4.6 x 250
4522-0202 5 Standard Analytical 4.6 x 150

*Requires one-time purchase of 4.6 mm ID WVS end fittings, Catalogue Number 4631-1001.
1Irregular Media
2 Spherical Media
Ordering Information
Catalogue Number Product Quantity/Pack
 
Guard Cartridge Holders (For use with WVS Analytical Column)

 
4631-1003 Integral Guard Cartridge Holder WVS Small 1

 
4631-1004 Universal Guard Cartridge Holder WCS Small 1
Guard Cartridge System Replacement Cartridges
4641-0001 SIL Cartridge 5
4641-0002 RP Cartridge 5
4641-0005 SAX Cartridge 5
4641-0007 SCX Cartridge 5
4641-0008 PAC Cartridge 5
PartiSphere RTF Guard Cartridges
4641-1002 PartiSphere RTF C-18 5
WVS Analytical Hardware
4631-1001 Column end fittings: WVS type - (for void sealing columns) 1 pair
 

HPLC