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Aquaporins (AQP0-10) And Urea
Transporter (UT2) Antibodies
Water is a critical component of all living
cells. Interestingly, tissue membranes show a great degree of water permeability.
Mammalian red cells, renal proximal tubules, and descending thin limb of Henle
are extraordinarily permeable to water. Water crosses hydrophobic plasma
membranes either by simple diffusion or through a facilitative transport
mechanism mediated by special protein "aquaporins". Over the last decade, genes
for several members of aquaporin family have been cloned, expressed, and their
distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26
kDa), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel
forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the
foundation of the growing family of aquaporin. The lens specific AQP0 represents
up to 80% of total lens membrane protein. AQP2 (AQP-CD or WCH-CD; 265 AA; gene
locus 12q13) is the vasopressin-regulated water channel of the apical membrane
of collecting duct cells. AQP3 (GLIP, Glycerol-transporting integral protein;
285 AA) has a wide tissue distribution. AQP4 (MIWC, mercury-insensitive water
channel; 301 AA; gene locus 18q22) is expressed in brain and in other tissues as
well. AQP5 (282 AA) is found in several epithelial tissues (salivary and
lachrymal glands, etc). AQP6 (WCH3 or hKID or AQP2-like; 282 aa; chromosome
12q13) is only found in kidney with low water permeability. AQP7 (rAQP7, 269aa)
is abundantly expressed in testis. It is involved in water, glycerol and urea
transport. AQP7 is also found in adipose tissue, kidney, and heart. AQP8 (263aa)
is also abundant in testis. It is also found in liver, pancreas, placenta and
salivary gland. AQP9 (295aa) is primarily expressed in peripheral leukocytes. It
is permeable to water and urea. AQP10 (human 264 aa) is most closely related
with AQP3 (53%), AQP9 (52%), and AQP7 (43%). It is abundantly expressed in
duodenum and jejunum. AQP10 is not permeable to urea and glycerol. Most recently,
adipose specific AQP-adipose (342aa; AQPAP or AQP7-like) has been cloned. It
facilities water and glycerol transport. The individual members of aquaporin
family have identical predicted secondary structures with up to 6 highly
conserved hydrophobic membrane spanning domains (about 18-25 AA each) and two
conserved NPA motifs. However, N/C-terminal regions of AQPs are only ~ 20%
conserved.
Urea is the major end product of nitrogen metabolism and it is concentrated up
to four times higher than that of plasma. A rapid, passive transport of urea
through the membranes of the IMCD is responsible for generation of high urea
concentration in the inner medullary interstitium. Recently, a
vasopressin-sensitive urea transporter (UT2; 397 AA) from rabbit and its homolog
from rat (rUT2; 397 AA) have been cloned.
ADI has produced highly specific rabbit antibodies to AQP0-10, AQPAP and rUT2
using peptide sequences specific to each AQP. These antibodies should be useful
in studying various aquaporins and rUT2.
|
Items |
Antigen
peptide |
Ab
Host |
Ab
Cross-
reactivity |
Neat
Antisera
(100 ul)
Cat # |
Aff.
Pure Ab
(100 ug)
Cat # |
* Control
Peptide
(100 ug)
Cat # |
|
hAQP0 |
h, 17 aa~CT |
Rb |
M, R, H, B |
AQP01-S |
AQP01-A |
AQP01-P |
|
hAQP0 |
h, 17 aa~CT |
Ch |
M, R, H, B |
AQP02-S |
AQP02-A |
AQP02-P |
|
rAQP1 |
r, 19 aa~CT |
Rb |
M, R, B, S |
AQP11-S |
AQP11-A |
AQP11-P |
|
rAQP2 |
r, 15 aa~CT |
Rb |
R, M, H, P |
AQP21-S |
AQP21-A |
AQP21-P |
|
AQP2 |
m, 19 aa, 2nd EC |
Rb |
m, r (O, h?) |
AQP23-S |
AQP23-A |
AQP23-P |
|
rAQP3 |
r, 15 aa~CT |
Rb |
M, R, H |
AQP31-S |
AQP31-A |
AQP31-P |
|
rAQP4 |
r, 17 aa~CT |
Rb |
R, M, H |
AQP41-S |
AQP41-A |
AQP41-P |
|
rAQP5 |
r, 17 aa~CT |
Rb |
R, M, H |
AQP51-S |
AQP51-A |
AQP51-P |
|
hAQP6 |
h, 19 aa~CT |
Rb |
H (M,
R?) |
AQP61-S |
AQP61-A |
AQP61-P |
|
hAQP6 |
h, 19
aa~CT |
Ch |
H (M,
R?) |
AQP62-S |
AQP62-A |
AQP62-P |
|
rAQP7 |
r, 18
aa~NT |
Rb |
R, M
(H?) |
AQP71-S |
AQP71-A |
AQP71-P |
|
rAQP7 |
r,
18 aa~NT |
Ch |
R,
M (H?) |
AQP72-S |
AQP72-A |
AQP72-P |
|
rAQP8 |
r, 16 aa~CT |
Rb |
R, M (H?) |
AQP81-S |
AQP81-A |
AQP81-P |
|
rAQP8 |
r, 16 aa~CT |
Ch |
R, M (H?) |
AQP82-S |
AQP82-A |
AQP82-P |
|
rAQP9 |
r, 18aa~CT |
Rb |
R (H?) |
AQP91-S |
AQP91-A |
AQP91-P |
|
rAQP9 |
r, 18aa~CT |
Ch |
R (H?) |
AQP92-S |
AQP92-A |
AQP92-P |
|
hAQPAP |
h, 20 aa~NT |
Rb |
H (M, R?) |
AQPAP11-S |
AQPAP11-A |
AQPAP11-P |
|
AQP10 |
H, 17 aA,
~CT |
Rb |
H (M, R?) |
AQP101-S |
AQP101-A |
AQP101-P |
|
hAQPAP |
h, 20
aa~NT |
Ch |
H (M,
R?) |
AQPAP12-S |
AQPAP12-A |
AQPAP12-P |
|
AQP1-9
Kit |
AQP
1-9 Antibody Kit, 25 ul of
individual Rabbit Antibodies as
Listed Above |
AQP19-PAN-S; |
AQP19-PAN-A; |
AQP19-PAN-P; |
|
rUT2 |
r,
19 aa~CT |
Rb |
R,
M, H |
RUT21-S |
RUT21-A |
RUT21-P |
|
Control
Rabbit IgG |
For use in ELISA, Western,
Immunohisto. |
20009-1 (1
mg) |
|
Control Chicken IgG |
For use in ELISA, Western,
Immunohisto. |
20010-1 (1
mg) |
M= Mouse; R=Rat; H=Human;
Ha=Hamster; Rb=Rabbit;
B=Bovine;
Ch=Chicken;
CT= near C-terminus; NT=near
N-terminus; Internal=Middle of
protein. EC=Extracellular
domain; IC=Intracellular
domain;
"Neat Antisera" are the
unpurified antiserum and it is
suitable for ELISA and
Western.
"Affinity pure" antibodies
have been over the
antigen-affinity column and
recommended for
immunohistochemical
applications, and may give
cleaner results in most
applications.
"Control peptides" can not be
used for Western as they are
very short peptides. They are
intended for ELISA or antibody
competition studies
All Products are for in vitro
research use only. Rev. 20319
List of publications using
ADI's antibodies for various
AQPs:
AQP1h Wang W 2003 Calcified
Tissue International in press,
Aquaporin Expression in
Developing Human Teeth and
Selected Orofacial Tissues, WB
bought AQP0-9, AQp1, 3, 5, and
6.
AQP1 Bates CM 2003 Kidney
International. 63(1):53-63,
Expression of somatostatin
receptors 3, 4, and 5 in mouse
kidney proximal tubules. IHC
mice kidney; somato. R 3-5
cap.
AQP1 Speake T 2003 BBA, 1609,
80-86, Expression of aquaporin
1 and aquaporin 4 water
channels in rat choroid
plexus, WB IF 4% PF rat brain
(choroid plexus).
AQP1 Gannon BJ et al, 2003
Lymphatic Research and Biology
1, 55-66, Endothelial
Distribution of the Membrane
Water Channel Molecule
Aquaporin-1: Implications for
Tissue and Lymph Fluid
Physiology?
IHC, rat intestines etc.
AQP1 Lai, KN 2002 Kidney
International 62 Issue 4 Page
1431 Expression of aquaporin-3
in human peritoneal
mesothelial cells and its
up-regulation by glucose in
vitro WB, IHC human cells.
AQP1 Furuya S 2002 Cell Tissue
Res. 308: 75-86, Distribution
of aquaporin 1 in the rat
pancreatic duct system
examined with light- and
electron-microscopic
immunohistochemistry, IHC EM
4% PF rat pancreas.
AQP1 Ko Shigeru B. H. 2002 Am.
J. Physiol 282, G324-G331,
Aquaporins in rat pancreatic
interlobular ducts IHC, aqp5
perosnal 4% PF rat pancreas.
AQP1 Amlal, H et al 2001 Am.
J. physiol. Renal Physiol.
280, 513F-523F Fasting
downregulates renal water
channel AQP2 and causes
polyuria WB, used aqp2 his own
made by genosys.
AQP1 Wen, Quan Diecke 2001
Experimental Biology and
Medicine 2001 226: 463-467,
Immunocytochemical
Localization of Aquaporin-1 in
Bovine Corneal Endothelial
Cells and Keratocytes IHC,
bovine corneal cells
paraformaldehyde fixed tissues
AQP1 Gannon BJ et al 2000 J.
Smooth Muscle Res. 36,
155-167, AQP-1 expression in
visceral smooth muscle cells
of female rat reproductive
tract IHC, female rat
reproductive tract, zamboni
fixative.
AQP1 Cowan,Chad A. 2000 Neuron
2000 26: 417, EphB2 Guides
Axons at the Midline and Is
Necessary for Normal
Vestibular Function WB, IP.
AQP2 Saito O 2003 Am J Physiol
Renal Physiol, 284, 1164-1170,
Expression of the
Prostaglandin F Receptor (FP)
gene along the mouse
genitourinary tract IHC rat
collectin ducts.
AQP2 Schneider A 2002 Am J
Physiol Renal Physiol : 284:
411 - 417, Differential,
inducible gene targeting in
renal epithelia, vascular
endothelium, and visceral
organs of Mx1Cre mice IHC
AQP2, AQP3? Combet, Sophie
2001 Am J Physiol Renal
Physiol,
10.1152/ajprenal.0139.2001,
Food Restriction Prevents
Age-Related Polyuria by a
Vasopressin-Dependent Membrane
Recruitment of Aquaporin-2 WB,
IHC.
AQP2, AQP3 Combet, Sophie 2001
Food Restriction Prevents
Age-Related Polyuria by a
Vasopressin-Dependent Membrane
Recruitment of Aquaporin-2, Am
J Physiol Renal Physiol,
10.1152/ajprenal.0139.2001 WB,
IHC.
AQP3 Barcroft LC 2003
Developmental Biology 256,
342-354 Aquaporin proteins in
murine trophectoderm mediate
transepithelial water
movements during cavitation WB,
IHC murine embro/IF.
AQP3h Wang W 2003 Calcified
Tissue International in press,
Aquaporin Expression in
Developing Human Teeth and
Selected Orofacial Tissues WB
bought AQP0-9, AQp1, 3, 5, and
6.
AQP3 de Baey, Annegret 2000 J.
Exp. Med. 2000 191: 743-748,
The Role of Aquaporins in
Dendritic Cell
Macropinocytosis WB, human
dendritic cells.
AQP4 Amiry-Moghaddam M 2003
PNAS, Feb 2003; 100: 2106 -
2111, An
alpha-syntrophin-dependent
pool of AQP4 in astroglial
end-feet confers bidirectional
water flow between blood and
brain IHC 4% PF mouse brain.
AQP4 Speake T 2003 BBA, 1609,
80-86 Expression of aquaporin
1 and aquaporin 4 water
channels in rat choroid
plexus, WB IF 4% PF rat brain
(choroid plexus).
AQP4 Price DL 2002 Brain
Research, Brain Research, 957,
Issue 1, 6 53-60 Distribution
of rSlo Ca2+-activated K+
channels in rat astrocyte
perivascular endfeet, IF/Confocal
microscopy, rat brain rat
brain.
AQP4 Yana, Yiping , 2001 Brain
Research, 17, 43-55 Expression
of Na+-K+-Cl- cotransporter in
rat brain during development
and its localization in mature
astrocytes IHC, 4% PF rat
brain.
AQP4 Lynn, BD et al 2001 Brain
Research, 898, 1-8 Enrichment
of neuronal and glial
connexins in the postsynaptic
density subcellular fraction
of rat brain IHC.
AQP5 Rabb, H/Soleimani M 2003
Kidney International.
63(2):600-606, February 2003
Acute renal failure leads to
dysregulation of lung salt and
water channels, WB rat kidney.
AQP5h Wang W 2003 Calcified
Tissue International in press
Aquaporin Expression in
Developing Human Teeth and
Selected Orofacial Tissues WB
bought AQP0-9, AQp1, 3, 5, and
6.
AQP5 Beroukas, Dimitra 2002
Laboratory Investigation
82:1547-1552 Selective
Down-Regulation of Aquaporin-1
in Salivary Glands in Primary
Sjögren's Syndrome.
AQP5 Borok Z et al 2002 Am J
Physiol Lung Cell Mol Physiol
282: L599-L608 "Alveolar
Epithelial Ion and Fluid
Transport, Na transport
proteins are expressed by rat
alveolar epithelial type I
cells" Wb IF AT1/2 cell and
rat lung.
AQP5 Beroukas, Dimitra 2001
Lancet 358, 1875-1876
Subcellular distribution of
aquaporin 5 in salivary glands
in primary Sjögren's syndrome,
IHC, pdf human labial salivary
glands, formaldehyde, paraffin.
AQP5 Borok, Zea 1998 Am. J.
Respir. Cell Mol. Bio. 1998
18: 554-561 Keratinocyte
Growth Factor Modulates
Alveolar Epithelial Cell
Phenotype In Vitro: Expression
of Aquaporin 5 WB, sam resutls
with ADI ab as in refs. 30 rat
AT2 cells.
AQP6h Wang W 2003 Calcified
Tissue International in press
Aquaporin Expression in
Developing Human Teeth and
Selected Orofacial Tissues WB
bought AQP0-9, AQp1, 3, 5, and
6.
AQP7 Liu Z et al 2002 PNAS 99,
6053-6058 Arsenite transport
by mammalian aquaglyceroporins
AQP7 and AQP9 WB, expression
in yeast cells.
AQP8 Barcroft LC 2003
Developmental Biology 256,
342-354 Aquaporin proteins in
murine trophectoderm mediate
transepithelial water
movements during cavitation WB,
IHC murine embro/IF.
AQP8 Hubert C et al 2002 J.
Biol. Chem 277: 22710-22717
Expression and Localization of
Aquaporin Water Channels in
Rat Hepatocytes. EVIDENCE FOR
A ROLE IN CANALICULAR BILE
SECRETION WB IHC/IF 1:2K-1:5K
rat hepatocytes.
AQP8 Huebert RC et al 2002 JBC
May 2002 277, 22710-22717
Expression and localization of
aquaporin water channels in
rat hepatocytes. Evidence for
a role in canalicular bile
secretion, WB, IHC, IP, AQP),
9-9, and others IF, rat
hepatocytes.
AQP8 Elkjær M-L et al 2002 Am
J Physiol Renal Physiol 2001
281: F1047-F1057
Immunolocalization of
aquaporin-8 in rat kidney,
gastrointestinal tract, testis,
and airways. WB, IHC, 4% pf/paraffin
rat kideny.
AQP8 Elkjaer, Marie-Louise
2001 Am J Physiol Renal
Physiol 0: 15820011-1 (in
press) IMMUNOLOCALIZATION OF
AQUAPORIN-8 IN RAT KIDNEY,
LIVER, TESTIS, EPIDIDYMIS,
JEJUNUM, COLON, PRINCIPAL
BRONCHI AND SALIVARY GLANDS.
IHC, made his own and compared
our antibodies IHC in rat
epidydamis, rat myoepithejila
cells.
AQP8 Calamita, Giuseppe 2001
Biol Reprod 2001 64: 1660-1666
Expression and Localization of
the Aquaporin-8 Water Channel
in Rat Testis WB, IHC, aqp7
from others 2%pf, reproducitve
tissue.
AQP8 Wellner RB 2000 Eur. J.
Physiol. 441, 49-56 Evidence
that aquaporin-8 is located in
the basolateral membrane of
rat submanidbular glad acinar
cells WB, IHC, wb antibodies
bind to glyco and non-glyco
forms.
AQP9 Barcroft LC 2003
Developmental Biology 256,
342-354 Aquaporin proteins in
murine trophectoderm mediate
transepithelial water
movements during cavitation WB,
IHC murine embro/IF.
AQP9 Okada S 2003 FEBS Letters,
Volume 540, Issues 1-3, 10
April 2003, Pages 157-162
Aquaporin-9 is expressed in a
mucus-secreting goblet cell
subset in the small intestine
WB, IHC mouse ileum, liver.
AQP9 Huebert RC et al 2002 JBC
May 2002 277, 22710-22717
Expression and localization of
aquaporin water channels in
rat hepatocytes. Evidence for
a role in canalicular bile
secretion. WB, IHC, IP, AQP),
9-9, and others IF, rat
hepatocytes.
AQP9 Liu Z et al 2002 PNAS 99,
6053-6058 Arsenite transport
by mammalian aquaglyceroporins
AQP7 and AQP9 WB, expression
in yeast cells.
AQP9 Hubert C et al 2002 J.
Biol. Chem 277: 22710-22717
Expression and Localization of
Aquaporin Water Channels in
Rat Hepatocytes. EVIDENCE FOR
A ROLE IN CANALICULAR BILE
SECRETION WB IHC/IF 1:2K-1:5K
rat hepatocytes.
AQP9 Nicchia GP 2001 J.
Histochem. Cytochem. 49:
1547-1556 Tissue Distribution
and Membrane Localization of
Aquaporin-9 Water Channel:
Evidence for Sex-linked
Differences in Liver WB, IHC,
4%pf, liver, brain.
UT-A1/A2 Hu M-C 2000 Massive
reduction of urea transporters
in remnnant kidney and brain
of uremic rat Kideny Intl. 58
1202-1210 WB, IHC, rat kidney/ut-b
sequence.
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