GeneXP™ kit
is Seegene’s original DSO™ -based multiplex
RT-PCR system.
- Only a single reaction in necessary to amplify
multiple genes of interest.
- One reaction is sufficient to amplify multiple
genes simultaneously in a single reaction.
GeneFishing™
DEG Dicovery kit uses
randomly designed arbitrary primers to
discover DEGs (differentially expressed
genes) in the samples, whereas GeneXP™
kit uses target specific primers for target
gene profiling.
Multiplex PCRs capacity to simultaneously
quantify miltiple mRNA species from a single
of RNA allows comparative analysis of
different mRNA species within samples.
Variations in RNA isolation, initial
quantification errors or tube-to-tube
variations in RT and PCR can be compensated
by including a known housekeeping gene. DSO™
primers can simultaneously detect multiple
target genes without creating any false
products.
Compare whith the other method To
date, gene expression patterns are often analyzed by
microarray methods that involve a DNA chip or by
PCR-based methods.
Microarray
Microarray analysis has the advantage over the other
methods in that it permits high throughput screening at
one time. However, it has many potential pitfalls, such
as a high false-positive rate and low sensitivity.
Moreover, the number of samples that can be compared is
restricted, and it is difficult to detect subtle
differences between the samples.
Experiments need to be repeated due to the low
reproducibility
Hybridization method is comparably lower in sensitivity
than PCR method
Additional experiments (Northern blot, Real-time PCR,
RT-PCR, etc.)are required
for validation.
All the clones with positive signal need to be checked,
hence requiring more
cost and time for confirmation steps
GeneXP™ Kit
Systematic Analysis of Gene Family Expression
High Specificity & Sensitivity using by DSO™-based
multiplex RT-PCR
No more confirmation step required
Comparison of normal vs. diseased tissue Ex. normal
vs. tumor, non-infected vs. infected, wild type vs.
knock-out
Time courses Ex.
developmental stage, environmental change
Test of influence of Chemical compounds Ex. Drug,
chemical, hormone, post-clinical treatments
Microarray data validation, etc.
Cat. No
Product
Size
HK16XX
GeneXP™ Human Prognosis
Signature Assay Kit (1~11)
Microarray analysis has the advantage over the other
methods in that it permits high throughput screening at
one time. However, it has many potential pitfalls, such
as a high false-positive rate and low sensitivity.
Moreover, the number of samples that can be compared is
restricted, and it is difficult to detect subtle
differences between the samples. 
