|
TEL: +32 16 58 90 45
Fax :+ 32 16 50 90 45
GENTAUR Europe
tel+32 2 732 5688
fax+32 2 732 4414
Av.
de l' Armée 68
B-1040 BRUSSELS
BELGIUM
GENTAUR France
tel 01 43 25
01 50
fax01 43 25
01 60
9, rue
Lagrange
75005 PARIS
FRANCE
GENTAUR Italy
tel 02 36 00
65 93
fax 02 36 00
65 94
20135 MILANO
ITALY
GENTAUR Germany
tel +49 241
6085 13140
fax +49 241
6085 33033
Forckenbeckstraße 6,
D-52074
Aachen
GERMANY
| |
Gc-globulin
Gc-globulin
– Key protein in the extracellular actin scavenger system.
Gc-globulin (Group-specific component globulin), also known as vitamin D binding
protein (DBP), is a multifunctional plasma glycoprotein. It has a molecular mass
of 51 kDa and is structually related to serum albumin1,2. One of the
most important functions of Gc-globulin is to act as an actin scavenger3.
Actin is the most abundant protein in eukaryotic cells and is released into
circulation by dying cells. Here it form filaments which may trigger
intravascular coagulation, if not rapidly removed4.
CLINICAL
SIGNIFICANCE
Formation of actin filaments in the bloodstream can lead to a condition
resembling multiple organ dysfunction syndrome (MODS)4. In the actin scavenger
system the actin filaments are depolymerized by gelsolin and the monomeric actin
is strongly bound by Gc-globulin. This consumes actin-free Gc-globulin. Low
levels of actin-free and total Gc-globulin can act as a prognostic marker in
situations of organ damage, such as fulminant hepatic failure5,6,
acetaminophen overdose7,8 and multiple trauma9,10. Septic
shock may also be associated with reduced Gc-globulin levels11,
though this have been less extensively studied.
PRODUCTS
ANTIBODYSHOP already
offer several monoclonal antibodies against the human Gc-globulin glycoprotein.
The HYB 249 series of antibodies was produced by immunizing mice with
Gc-globulin isolated from human plasma, conjugated to a carrier.
HYB 249-01 reacts alone with actin-free Gc-globulin.
HYB 249-02 and -05 reacts equally well with actin free Gc-globulin and
Gc-globulin with bound actin.
Code No.
|
Product description
|
Conjugation*
|
|
56-HYB
249-01 |
Mouse
monoclonal anti-Gc-globulin antibody |
B |
|
56-HYB
249-02 |
Mouse
monoclonal anti-Gc-globulin antibody |
B |
|
56-HYB
249-05 |
Mouse
monoclonal anti-Gc-globulin antibody |
- |
|
56-HYB
249-10 |
Mouse
monoclonal anti-Gc-globulin antibody |
- |
* B: biotin
APPLICATION
*The user should
determine the best combination of antibodies for their own application; ND: not
determined.
REFERENCES
|
1 |
Haddad JG (1995) Plasma vitamin D-binding
protein (Gc-globulin): multiple tasks. J Steroid Biochem Molec Biol
53:579-582. |
|
2 |
White P, Cooke N (2000) The multifunctional
properties and characteristics of vitamin D-binding protein. Trends
Endocrinol Metabol 11:320-327. |
|
3 |
Harper KD, McLeod JF,
Kowalski MA, Haddad JG (1987) Vitamin D binding protein sequesters monomeric
actin in the circulation of the rat. J Clin Invest 79:1365-1370. |
|
4 |
Haddad JG, Harper KD, Guoth
M, Pietra GG, Sanger JW (1990) Angiopathic consequences of saturating the
plasma scavenger system for actin. Proc Natl Acad Sci
USA
87:1381-1385 |
|
5 |
Schiødt F, Bondesen S, Petersen I, Dalhoff K,
Ott P, Tygstrup (1996) Admission levels of serum Gc-globulin: predictive
value in fulminant hepatic failure. Hepatology 23:713-718 |
|
6 |
Schiødt FV, Ott P, Bondesen S, Tygstrup N (1997)
Reduced serum Gc-globulin levels in patients with fulminant hepatic failure:
association with multiple organ failure. Crit Care Med 25:1366-1370 |
|
7 |
Schiødt FV, Bondesen S, Tygstrup N (1995) Serial
measurements of serum Gc-globulin in acetaminophen intoxication. Eur J
Gastroenterol Hepatol 7:635-640 |
|
8 |
Schiødt FV, Ott P, Tygstrup N, Dahl B, Bondesen
S (2001) Temporal profile of total, bound, and free Gc-globulin after
acetaminophen overdose. Liver Transpl 7:732-738 |
|
9 |
Dahl B. Schiødt FV, Nielsen M, Kiær T, Williams
JG, Ott P (1999) Admission levels of Gc-globulin predicts outcome after
multiple trauma. Injury 30:275-281. |
|
10 |
Dahl B, Schiodt FV, Ott P,
Wians F, Lee WM, Balko J, O'Keefe GE (2003) Plasma concentration of
Gc-globulin is associated with organ dysfunction and sepsis after injury.
Crit Care Med 31:152-156. |
|
11 |
Lee WM, Reines D, Watt GH, Cook JA, Wise WC,
Halushka PV, Galbraith RM (1989) Alterations in Gc levels and complexing in
septic shock. Circ Shock 28:249-255. |
|
