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Fluorescent Calcium Indicators and Related Products > Fluo-3

Fluo-3 has its absorption maximum at 506 nm, thus making it excitable by the 488 argon-ion laser. Unlike fura-2 and indo-1, neither the excitation nor the emission maximum of the sensor shifts significantly before and after Ca2+ binding. As a result, the ratioing technique is not applicable to fluo-3. Fluo-3 is essentially nonfluorescent without Ca2+ present, but the fluorescence increases at least 40 times on Ca2+ binding. Also, because fluo-3 binds Ca2+ more weakly (higher Kd) than do fura-2 and indo-1, it is more useful for measuring high transient Ca2+ concentration during Ca2+ spikes. A major application of fluo-3/AM is high throughput drug screening. The quality of Biotium's fluo-3/AM is among the best (395% by HPLC) while its price is the most competitive.
Ref.: Minta, A., et al. J. Biol. Chem. 264, 8171(1989); Kao, J.P.Y., et al. J. Biol. Chem. 264, 8179(1989).
   
 
1 - 7 of 7
Cat#
Product Name
Unit Size
Price Per Unit *
50010
Fluo-3, pentaammonium salt
1mg

180 €

50011
Fluo-3, pentapotassium salt
1mg

180 €

50012
Fluo-3, pentasodium salt
1mg

180 € 

50013
Fluo-3, AM ester
10x100ug

210 € 

50014
Fluo-3, AM ester
1mg

190 € 

50015
Fluo-3, AM ester, 1mM in anhydrous DMSO
1.0mL

210 €  

50016
Fluo-3, AM ester (special packaging)
20x50ug

235 €  

   
 
1 - 7 of 7
   
 
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