FGF 2 H RECOMBINANT (100)
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Product Details: Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT)

Basic Information

 
Product ID: GF-030-5
Product Name: Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT)
Unit: 100µg
Price: 515 Euro
Availability: Available
Category: Growth Factors, Cytokines, Receptors and their Antibodies
Subcategory: Growth Factors/Cytokines/Enzymes

 

Product Details

 
Description: Human Basic Fibroblast Growth Factor is a mitogen that stimulates cell growth for such cells as fibroblasts, endothelial cells, myoblasts, glial cells and smooth muscle cells. Basic FGF is composed of 146 amino acid residues (pro 1 to ser 146), and is 55% homologous with acidic FGF, including two conserved cysteine residues.

Human basic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.

 
Molecular Weight: 17.5 KDa
Biological Activity: The mitogenic activity of human basic FGF is identical to natural bovine basic FGF, as measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.

 
Formulation: Lyophilized powder
Purity: Over 95% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
Storage Conditions: Store at +4°C. Reconstitution is best in water or buffer near neutral pH, since it is unstable in acidic solutions. After reconstitution, storage in aliquots at -80°C is recommended.
Stability: Several months, lyophilized or in solution, at pH 6-7 and +4°C.
Temperature: +4°C
References: 1. Gospodarowicz, D. (1975) J. Biol. Chem. 250, 2515-2520.
2. Folkman, J. and Klagsbrun, M. (1987) Science 235,442-447
3. Fox, J., et al. (1996) Journal of Biological Chemistry 271: 12578-12584.